Antibody:	HTm4 antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS12: blood island	Present		3C
TS12: extraembryonic component	Present	Regionally restricted	3C	Expression is in the vessels.

Structure	Level	Pattern	Image	Note
TS22: liver	Present	Regionally restricted	3D	Expression is in hematopoietic cells (mononuclear cells and in some megakaryocytes.).

Structure	Level	Pattern	Image	Note
TS23: brain	Present	Regionally restricted	3E	Expression is in regions outside the ventricular zone.
TS23: retina layer	Weak	Regionally restricted	3E	Expression is in the ganglion cell layer.
TS23: dorsal root ganglion	Weak		3E
TS23: pretectum	Weak		3E

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	3F	Expression is in regions outside the ventricular zone.
TS20: forebrain	Present	Regionally restricted	3F	Expression is dorsal and is weak in the anterior part.
TS20: midbrain	Present		3F
TS20: midbrain-hindbrain junction	Present		3F
TS20: pons	Present	Regionally restricted	3F	Expression is in the pontine plate.
TS20: metencephalon alar plate	Present	Regionally restricted	3F	Expression is in the cerebellar plate.
TS20: spinal cord	Present	Regionally restricted	3F	Expression is ventral.
TS20: dorsal root ganglion	Weak	Regionally restricted	3F
TS20: sympathetic ganglion	Weak	Regionally restricted	3F

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present		3G

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present		3H

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present		3I

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present		3J

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present		3K

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Strong		3L
TS23: spinal cord	Strong		3L
TS23: spinal ganglion	Strong	Regionally restricted	3L	Expression is in ventral ganglia.
TS23: peripheral nerve	Present		3L
TS23: sympathetic ganglion	Present		3L

Structure	Level	Pattern	Image	Note
TS28: spleen	Strong		4B	Expression is in the cytoplasm of hematopoietic cells.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		4C

Structure	Level	Pattern	Image	Note
TS28: lymph node germinal center	Present		4D

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Absent

Structure	Level	Pattern	Image	Note
TS14: central nervous system	Absent

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present	Regionally restricted		Expression is at the periphery of the neural tube at all levels.

Structure	Level	Pattern	Image	Note
TS18: forebrain	Present	Regionally restricted		Expression is dorsal.
TS18: midbrain	Present
TS18: midbrain-hindbrain junction	Present
TS18: metencephalon alar plate	Present	Regionally restricted		Expression is in the cerebellar plate.
TS18: metencephalon basal plate	Present	Regionally restricted		Expression is in the pontine plate.
TS18: future spinal cord	Present	Regionally restricted		Expression is ventral.
TS18: dorsal root ganglion	Weak
TS18: sympathetic ganglion	Weak

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Regionally restricted		Expression is rostral.
TS21: diencephalon	Present	Regionally restricted		Expression is ventral to the neuroepithelium, and along the peripheral (subpial) regions caudally, corresponding to dorsal and ventral gray matter columns.
TS21: peripheral nerve	Weak	Regionally restricted		Expression is in the peripheral nerve roots.

Structure	Level	Pattern	Image	Note
TS22: spleen primordium	Present	Regionally restricted		Expression is in splenic red pulp hematopoietic cells. Absent in the non-germinal center B-cells.
TS22: spinal cord	Strong	Regionally restricted		Expression is ventral.
TS22: telencephalon	Strong	Regionally restricted		Expression is ventral.
TS22: diencephalon	Strong
TS22: medulla oblongata	Strong
TS22: midbrain tegmentum	Strong
TS22: pons	Strong
TS22: brain ventricular layer	Absent
TS22: eye	Absent
TS22: tectum	Absent

Structure	Level	Pattern	Image	Note
TS23: spleen primordium	Present	Regionally restricted		Expression is in splenic red pulp hematopoietic cells and in scattered cortical thymocytes.
TS23: spinal cord	Present	Regionally restricted		Expression is ventral.
TS23: telencephalon	Present	Regionally restricted		Expression is ventral.
TS23: diencephalon	Present
TS23: medulla oblongata	Present
TS23: midbrain tegmentum	Present
TS23: peripheral nervous system	Present
TS23: pons	Present
TS23: 4th ventricle	Present	Regionally restricted		Expression is in the ependyma.
TS23: enteric nervous system	Present	Regionally restricted		Expression is in the myenteric plexus.
TS23: pretectum	Weak
TS23: brain ventricular layer	Absent
TS23: tectum	Absent

Structure	Level	Pattern	Image	Note
TS24: spleen	Present	Regionally restricted		Expression is in splenic red pulp hematopoietic cells an in scattered cortical thymocytes.
TS24: brain subventricular zone	Present
TS24: caudate-putamen	Present
TS24: main olfactory bulb	Present
TS24: brainstem nucleus	Present	Regionally restricted		Expression is in the anterior and posterior pontine nuclear groups and the superior central raphe.
TS24: tectum	Present	Regionally restricted		Expression is in the superior tectal neuroepithelium.
TS24: midbrain	Present	Regionally restricted		Expression is in the reticular formation.
TS24: pons	Present	Regionally restricted		Expression is in the reticular formation.

Structure	Level	Pattern	Image	Note
TS28: Peyer's patch	Present
TS28: cerebral cortex	Present
TS28: hypothalamus	Present
TS28: thalamus	Present
TS28: hippocampus	Weak