Gcg Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:152922 Golson ML, et al., Jagged1 is a competitive inhibitor of Notch signaling in the embryonic pancreas. Mech Dev. 2009 Aug-Sep;126(8-9):687-99
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5827902
Gene symbol:	Gcg
Gene name:	glucagon

Antibody:	anti-Glucagon
Detection system:	Cy3

Results

1G ©

1J ©

Specimen 1G/J: embryonic day 14.5 (more

)
Note: Triple labeled: red - Gluc (Gcg); green - Jag1 (assay MGI:5827901); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: pancreas parenchyma	Present	Regionally restricted	1J , 1G	Expression in differentiated alpha cells.

Specimen E16.5 not shown: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: endocrine pancreas	Present	Regionally restricted		Expression in differentiated endocrine cells.

3A ©

Specimen 3A: postnatal day 21; Jag1^tm1.1Loo/Jag1^tm1.1Loo (more

)
Note: Authors state that littermate Jag1loxP/loxP and Jag1loxP/+ mice were used as controls in most experiments. Double labeled: red - Gluc (Gcg); green - Ins1 (assay MGI:5827906) and Ins2 (assay MGI:5827907).

Structure	Level	Pattern	Image	Note
TS28: pancreas parenchyma	Present	Regionally restricted	3A

3B ©

Specimen 3B: postnatal day 21; Jag1^tm1.1Loo/Jag1^tm1.1Loo, Tg(Foxa3-cre)1Khk/0 (more

)
Note: Conditional mutant. Double labeled: red - Gluc (Gcg); green - Ins1 (assay MGI:5827906) and Ins2 (assay MGI:5827907).

Structure	Level	Pattern	Image	Note
TS28: pancreas parenchyma	Present	Regionally restricted	3B	Authors report an approximate 2.5-fold increase in alpha-cell mass by P21 in mutants vs. controls.

6E ©

Specimen 6E: embryonic day 16.5; Jag1^tm1.1Loo/Jag1^tm1.1Loo (more

Structure	Level	Pattern	Image	Note
TS24: pancreas parenchyma	Present	Regionally restricted	6E

6F ©

Specimen 6F: embryonic day 16.5; Jag1^tm1.1Loo/Jag1^tm1.1Loo, Tg(Foxa3-cre)1Khk/0 (more

)
Note: Conditional mutant. Double labeled: red - Gluc (Gcg); green - Ins1 (assay MGI:5827906) and Ins2 (assay MGI:5827907).

Structure	Level	Pattern	Image	Note
TS24: pancreas parenchyma	Present	Regionally restricted	6F	Authors report that the number of expressing cells was reduced by approximately one-half.

Specimen E18.5 Control not shown: embryonic day 18.5; Jag1^tm1.1Loo/Jag1^tm1.1Loo (more

)
Note: Authors state that littermate Jag1loxP/loxP and Jag1loxP/+ mice were used as controls in most experiments.

Structure	Level	Pattern	Image	Note
TS26: pancreas	Present

Specimen E18.5 Mutant not shown: embryonic day 18.5; Jag1^tm1.1Loo/Jag1^tm1.1Loo, Tg(Foxa3-cre)1Khk/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: pancreas	Present

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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