Jag1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:152922 Golson ML, et al., Jagged1 is a competitive inhibitor of Notch signaling in the embryonic pancreas. Mech Dev. 2009 Aug-Sep;126(8-9):687-99
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5827901
Gene symbol:	Jag1
Gene name:	jagged 1

Antibody:	anti-Jagged1(C-20): sc-6011
Detection system:	Cy2
Assay notes:	Authors describe two different Jag1 antibodies. Jagged1 antibody (MGI:5827806) may have been used.

Results

1D ©

1F ©

Specimen 1D/F: embryonic day 12.5 (more

)
Note: Triple labeled: green - Jag1: red - Pdx1 (assay MGI:5827900); blue - Dapi.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	1D , 1F	Expression in undifferentiated pancreatic epithelium.

Specimen E14.5 not shown: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Present	Regionally restricted		Expression in epithelial pancreatic precursor cells.

1H ©

1J ©

Specimen 1H/J: embryonic day 14.5 (more

)
Note: Triple labeled: green - Jag1: red - Gluc (Gcg assay MGI:5827902); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: pancreas parenchyma	Present	Regionally restricted	1H , 1J	Expression in differentiated alpha cells.

Specimen E16.5 not shown: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: pancreas parenchyma	Present	Regionally restricted		Authors report expression in some but not all cells positive for markers of differentiated pancreatic cells types, and for the exocrine marker amylase.

1L ©

1N ©

Specimen 1L/N: embryonic day 18.5 (more

)
Note: Triple labeled: green - Jag1: red - DBA (lectin); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: pancreatic duct	Present		1L , 1N	Expression limited to duct cells.

1P ©

1R ©

Specimen 1P/R: embryonic day 18.5 (more

)
Note: Triple labeled: green - Jag1: red - Ins1 (assay MGI:5827903) and Ins2 (assay MGI:5827904); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: islets of Langerhans	Present	Regionally restricted	1R , 1P	Expression limited to differentiated islet cells

2D ©

2F ©

Specimen 2D/F: embryonic day 18.5; Jag1^tm1.1Loo/Jag1^tm1.1Loo (more

)
Note: Authors state that littermate Jag1loxP/loxP and Jag1loxP/+ mice were used as controls in most experiments. Triple labeled: green - Jag1; red - E-Cad (Cdh1 assay MGI:5827911); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: islets of Langerhans	Present	Regionally restricted	2F , 2D	Expression in epithelial islet clusters.

2H ©

2J ©

Specimen 2H/J: embryonic day 18.5; Jag1^tm1.1Loo/Jag1^tm1.1Loo, Tg(Foxa3-cre)1Khk/0 (more

)
Note: Conditional mutant. Triple labeled: green - Jag1; red - E-Cad (Cdh1 assay MGI:5827911); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: islets of Langerhans	Absent		2J , 2H

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