Probe:	Bft cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: limb bud	Present	Regionally restricted	4A	Expressed in two lateral stripes at the posterior region of the body, corresponding to the expected sites for the outgrowth of hindlimb buds.

Structure	Level	Pattern	Image	Note
TS17: genital swelling	Present		4B	Most strongly expressed.
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	4B	Most strongly expressed. Expression was restricted to a rostral compartment.
TS17: hindlimb bud	Present	Widespread	4B	Most strongly expressed.
TS17: forelimb bud	Absent		4B
TS17: nasal pit	Present		4B
TS17: otocyst	Present		4B

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	4C	Expressed in the proximal region.
TS19: body wall	Present	Regionally restricted	4C	Segmented expression close to the hindlimb buds on the ventral region.
TS19: mandibular process	Present		4C

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present	Regionally restricted	4D	Expressed in a net-like pattern.
TS21: forelimb	Absent		4D

Structure	Level	Pattern	Image	Note
TS21: genital swelling of genital tubercle	Present		4E
TS21: remnant of Rathke's pouch	Present		4E
TS21: tongue	Present		4E

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present	Regionally restricted	5A	Expressed in a net-like pattern.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit mesenchyme	Present	Regionally restricted	5B	Expressed underneath the epithelial cells at the tips of digits.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit mesenchyme	Present	Regionally restricted	5C	Expressed in the mesenchyme between cartilages and bones.
TS22: hindlimb cartilage condensation	Absent		5C

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit mesenchyme	Present	Regionally restricted	5D	Expressed in the mesenchyme surrounding the digital condensation regions.
TS22: hindlimb cartilage condensation	Absent		5D

Structure	Level	Pattern	Image	Note
TS22: hindlimb mesenchyme	Strong	Regionally restricted	5E	Expressed at the end of long bones.
TS22: hindlimb cartilage condensation	Absent		5E

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Weak

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted		The strongest expression was in the dorsal-proximal region.