Gene Expression Data
Assay Details
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Assay
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Reference:
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J:83705
Kawamura K, et al., Ghrelin inhibits the development of mouse preimplantation embryos in vitro. Endocrinology. 2003 Jun;144(6):2623-33
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:5811295
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Gene symbol:
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Ghsr
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Gene name:
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growth hormone secretagogue receptor
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Probe:
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GHSR1a-pC, GHSR1a-pD
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Assay notes:
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Hemi-nested PCR was performed with first amplification using primers GHSR1a-pA, GHSR1a-pB (MGI:5811290, expected size of product 375 bp) and 2nd amplification using primers GHSR1a-pC, GHSR1a-pD (MGI:5811291, expected size of product 354 bp).
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Results
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Image: 1_GHSR
| Mol. Wt. Marker Lane |
| P adult
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TS28: brain
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Present
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Not Specified; poly-A+ RNA |
IVCS |
| Female |
| Control |
| P adult
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TS28: secondary oocyte
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Absent
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Not Specified; poly-A+ RNA |
Not Specified |
| Female |
| E0.5
(a)
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TS1: 1-cell stage embryo
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Absent
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Not Specified; poly-A+ RNA |
IVCS |
| Not Specified |
| E1.0
(a)
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TS2: 2-cell stage embryo
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Absent
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Not Specified; poly-A+ RNA |
IVCS |
| Not Specified |
| In vitro data: no data stored |
| In vitro data: no data stored |
| In vitro data: no data stored |
| In vitro data: no data stored |
| In vitro data: no data stored |
Notes:
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Analysis Tools
