Antibody:	P2X3 antibody
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present		1B
TS15: future forebrain	Present		1B
TS15: future hindbrain	Present		1B
TS15: future midbrain	Present		1B
TS15: midbrain-hindbrain junction	Present		1B

Structure	Level	Pattern	Image	Note
TS17: diencephalon marginal layer	Present	Regionally restricted	1C	Expression is in neurofibers.
TS17: hindbrain marginal layer	Present	Regionally restricted	1C	Expression is in neurofibers.
TS17: midbrain marginal layer	Present	Regionally restricted	1C	Expression is in neurofibers.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	1D	Expression is in marginal layer of the alar and basal plates.

Structure	Level	Pattern	Image	Note
TS19: neural tube alar columns	Present	Regionally restricted	1E	Expression is in marginal layer.
TS19: neural tube basal columns	Present	Regionally restricted	1E	Expression is in marginal layer.
TS19: dorsal root ganglion	Present	Uniform	1E

Structure	Level	Pattern	Image	Note
TS22: alar columns	Present		1F
TS22: basal columns	Absent		1F

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	2B	Expression is in the facial (VII) motor nucleus.

Structure	Level	Pattern	Image	Note
TS22: brainstem	Present	Regionally restricted	2D	Expression is in the mesencephalic trigeminal nucleus.

Structure	Level	Pattern	Image	Note
TS22: neural retina	Weak		2E
TS22: optic nerve	Weak		2E

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present		3B
TS19: facial ganglion	Present		3B

Structure	Level	Pattern	Image	Note
TS17: glossopharyngeal ganglion	Present	Regionally restricted	3D	Expression is in the inferior and superior ganglia including strong fiber staining along the esophagus.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present	Uniform	3E

Structure	Level	Pattern	Image	Note
TS22: intestine wall	Strong	Regionally restricted	4B , 4A	Expression is in neurofibers.
TS22: stomach	Strong	Regionally restricted	4C , 4E , 4A	Expression is in neurofibers in the wall.
TS22: blood vessel	Strong	Regionally restricted	4A , 4D	Expression is in neurofibers in the wall.

Structure	Level	Pattern	Image	Note
TS22: lung	Strong	Regionally restricted	4F	Expression is in neurofibers.

Structure	Level	Pattern	Image	Note
TS22: vibrissa follicle	Strong	Regionally restricted	4G	Expression is in neurofibers.

Structure	Level	Pattern	Image	Note
TS22: thoracic ganglion	Present		5B

Structure	Level	Pattern	Image	Note
TS22: inferior vagus ganglion	Present		5C
TS22: superior cervical ganglion	Absent		5C

Structure	Level	Pattern	Image	Note
TS22: adrenal medulla	Present	Regionally restricted	5E	Expression is in the chromaffin cells.

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch mesenchyme	Strong	Regionally restricted	6A	Expression is in the subepidermal region.

Structure	Level	Pattern	Image	Note
TS15: hindlimb ridge	Strong		6B

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Leydig cell	Leydig cell	Present		6C , 6D

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Present		6E

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Strong
TS15: facio-acoustic preganglion complex	Strong
TS15: glossopharyngeal preganglion	Strong
TS15: surface ectoderm	Strong
TS15: trigeminal preganglion	Strong
TS15: vagus preganglion	Strong
TS15: brain ventricle	Moderate
TS15: branchial arch	Moderate
TS15: limb	Moderate
TS15: tail	Moderate
TS15: optic cup	Absent			Absent in the retina and optic nerve.

Structure	Level	Pattern	Image	Note
TS16: dorsal root ganglion	Strong
TS16: glossopharyngeal ganglion	Strong
TS16: trigeminal ganglion	Strong
TS16: vagus ganglion	Strong
TS16: surface ectoderm	Moderate
TS16: future hindbrain	Weak	Regionally restricted		Expression is in the marginal layer of the myelencephalon.
TS16: brain ventricle	Absent

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Strong
TS17: diencephalon marginal layer	Strong
TS17: dorsal root ganglion	Strong
TS17: facial ganglion	Strong
TS17: glossopharyngeal ganglion	Strong
TS17: midbrain marginal layer	Strong
TS17: sympathetic nerve trunk	Strong
TS17: trigeminal ganglion	Strong
TS17: vagus ganglion	Strong
TS17: neural tube lateral wall	Strong	Regionally restricted		Expression is in the marginal layer of the alar and basal plates and in motor neurons.
TS17: hindbrain marginal layer	Strong	Regionally restricted		Expression is in the marginal layer of the myelencephalon.
TS17: brain	Strong	Regionally restricted		Expression is in the mesencephalic trigeminal nucleus.
TS17: peripheral nervous system	Strong	Regionally restricted		Strong in the sympathetic trunk and in fibers in internal organs and is absent in the sympathetic ganglia.
TS17: dorsal aorta	Moderate	Regionally restricted		Expression is in the wall.
TS17: surface ectoderm	Weak
TS17: brain ventricle	Absent
TS17: branchial arch	Absent
TS17: limb	Absent
TS17: retina	Absent
TS17: tail	Absent

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Strong
TS19: dorsal root ganglion	Strong
TS19: facial ganglion	Strong
TS19: glossopharyngeal ganglion	Strong
TS19: midbrain marginal layer	Strong
TS19: myelencephalon marginal layer	Strong
TS19: neural tube marginal layer	Strong
TS19: trigeminal ganglion	Strong
TS19: vagus ganglion	Strong
TS19: neural tube alar columns	Strong	Regionally restricted		Expression is in the marginal layer.
TS19: neural tube basal columns	Strong	Regionally restricted		Expression is in the marginal layer.
TS19: brain	Strong	Regionally restricted		Expression is in the mesencephalic trigeminal nucleus and the facial motor nucleus.
TS19: peripheral nervous system	Strong	Regionally restricted		Expression is in fibers in internal organs.
TS19: dorsal aorta	Moderate	Regionally restricted		Expression is in the wall.
TS19: diencephalon lateral wall marginal layer	Weak
TS19: retina	Weak
TS19: surface ectoderm	Weak
TS19: brain ventricle	Absent
TS19: branchial arch	Absent
TS19: limb	Absent
TS19: tail	Absent

Structure	Cell Type	Level	Pattern	Image	Note
TS22: adrenal medulla		Strong
TS22: dorsal root ganglion		Strong
TS22: facial ganglion		Strong
TS22: glossopharyngeal ganglion		Strong
TS22: medulla oblongata marginal layer		Strong
TS22: trigeminal ganglion		Strong
TS22: vagus ganglion		Strong
TS22: vestibulo-cochlear ganglion		Strong
TS22: vibrissa follicle		Strong
TS22: alar columns		Strong	Regionally restricted		Expression is in the marginal layer.
TS22: brainstem		Strong	Regionally restricted		Expression is in the mesencephalic trigeminal nucleus and the facial motor nucleus.
TS22: peripheral nervous system		Strong	Regionally restricted		Expression is in fibers in internal organs.
TS22: epidermis		Weak
TS22: midbrain marginal layer		Weak
TS22: sympathetic ganglion		Weak
TS22: sympathetic nerve trunk		Weak
TS22: Leydig cell	Leydig cell	Strong
TS22: basal columns		Absent
TS22: brain ventricle		Absent
TS22: diencephalon lateral wall marginal layer		Absent
TS22: dorsal aorta		Absent
TS22: limb		Absent
TS22: tail		Absent

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted		Expression is in a subpopulation of small-diameter neurons.
TS28: trigeminal ganglion	Present	Regionally restricted		Expression is in a subpopulation of small-diameter neurons.