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Gene Expression Data
Assay Details
Assay
Reference: J:213870 Chen J, et al., mTORC1 signaling controls mammalian skeletal growth through stimulation of protein synthesis. Development. 2014 Jul;141(14):2848-54
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5806817
Gene symbol: Rps6
Gene name: ribosomal protein S6
Antibody: phospho-RPS6 (ser240/244) antibody (#2215)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1A: embryonic day 15.0 (more )
Structure Level Pattern Image Note
TS23: humerus Present Punctate 1A , 1A right top , 1A right middle , 1A right bottom Expression is among the round chondrocytes, is stronger and more uniform in the columnar region in prehypertrophic and early hypertrophic chondrocytes, and is weaker in most of the hypertrophic region. Expression was reactivated in cells at the final stage of hypertrophy.
Specimen 1C: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: humerus Present Regionally restricted 1C right middle , 1C , 1C right top , 1C right bottom Expression is among the round chondrocytes, is stronger and more uniform in the columnar region in prehypertrophic and early hypertrophic chondrocytes, and is weaker in most of the hypertrophic region. Expression is also in terminal hypertrophic chondrocytes near the chondro-osseous junction.
Specimen S2B WT: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: humerus Present S2B WT Expression is in chondrocytes and osteoblast precursors.
Specimen S2B RapCKO: embryonic day 16.5; Rptortm1.1Dmsa/Rptortm1.1Dmsa, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: humerus Absent S2B RapCKO
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory