Probe:	Lzts2 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Similar staining patterns were observed in E9.5-12.5 embryos with another probe. MGI:5806250

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Widespread	1A E9.5 AS
TS15: 1st branchial arch mandibular component	Strong		1A E9.5 AS
TS15: forelimb bud	Strong		1A E9.5 AS
TS15: future forebrain	Strong		1A E9.5 AS
TS15: future hindbrain	Strong		1A E9.5 AS
TS15: future midbrain	Strong		1A E9.5 AS
TS15: tail	Present		1A E9.5 AS
TS15: head	Present	Regionally restricted	1A E9.5 AS	Craniofacial expression was apparent.

Structure	Level	Pattern	Image	Note
TS17: tail	Present	Regionally restricted	1A E10.5 AS	Expression appeared to become more localized to the periphery of the embryo.
TS17: head	Present	Regionally restricted	1A E10.5 AS	Craniofacial expression was apparent.
TS17: limb	Present		1A E10.5 AS

Structure	Level	Pattern	Image	Note
TS19: tail	Present	Regionally restricted	1A E11.5 AS	Expression appeared to become more localized to the periphery of the embryo.
TS19: face	Present	Regionally restricted	1A E11.5 AS	Craniofacial expression was apparent and became more localized to the periphery of the embryo as development progressed.
TS19: limb	Present		1A E11.5 AS

Structure	Level	Pattern	Image	Note
TS20: tail	Present	Regionally restricted	1A E12.5 AS	Expression appeared to become more localized to the periphery of the embryo.
TS20: face	Present	Regionally restricted	1A E12.5 AS	Craniofacial expression was apparent and became more localized to the periphery of the embryo as development progressed.
TS20: limb	Present		1A E12.5 AS

Structure	Level	Pattern	Image	Note
TS18: nephric duct	Present		4B/B'

Structure	Level	Pattern	Image	Note
TS18: nephric duct	Absent		4C/C'

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present	Regionally restricted	4E	Expression was detected in ureteric bud and mesenchyme-derived tissues in the kidney.
TS22: adrenal gland	Present		4E
TS22: bladder	Present		4E
TS22: testis	Present		4E

Structure	Level	Pattern	Image	Note
TS22: embryo	Absent		4F

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Present		4H	Expression appeared more concentrated in ureteric buds and collecting ducts.
TS23: cap mesenchyme	Present		4H
TS23: renal vesicle	Present		4H

Structure	Level	Pattern	Image	Note
TS23: cap mesenchyme	Absent		4I
TS23: collecting duct	Absent		4I
TS23: renal vesicle	Absent		4I

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Present		4K	Expression appeared more concentrated in ureteric buds and collecting ducts.
TS23: cap mesenchyme	Present		4K
TS23: renal vesicle	Present		4K

Structure	Level	Pattern	Image	Note
TS23: cap mesenchyme	Absent		4L
TS23: collecting duct	Absent		4L
TS23: renal vesicle	Absent		4L

Structure	Level	Pattern	Image	Note
TS26: metanephros	Present		4M

Structure	Level	Pattern	Image	Note
TS26: metanephros	Present		4N	Expression was decreased.

Structure	Level	Pattern	Image	Note
TS26: metanephros	Absent		4O