Probe:	NCAM MSD probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: myotome	Absent		3D	No expression at this stage, even in the well-developed myotome.
TS17: cardiac muscle tissue	Absent		3D	No expression in cardiac myocytes.
TS17: forelimb bud	Absent		3D	No expression in myogenic cells migrating into early limb buds.
TS17: hindlimb bud	Absent		3D	No expression in myogenic cells migrating into early limb buds.

Structure	Level	Pattern	Image	Note
TS19: myotome	Trace		4E	Expression in myotomes at level just above background.
TS19: neural tube	Absent		4E	No expression in neural tube

Structure	Level	Pattern	Image	Note
TS20: trunk musculature	Strong	Regionally restricted	5C	Higher level of expression in vertebral musculature and intercostal muscles than at E11.5.
TS20: limb mesenchyme	Present	Regionally restricted	5C	Expression in premuscle masses in the developing limbs.

Structure	Level	Pattern	Image	Note
TS21: trunk musculature	Present	Regionally restricted	6C	Expression in abdominal wall and back muscles.
TS21: hindlimb	Present		6C	Expression in hindlimb.
TS21: spinal cord	Absent		6C	No expression in spinal cord or spinal ganglia.
TS21: spinal ganglion	Absent		6C	No expression in spinal cord or spinal ganglia.

Structure	Level	Pattern	Image	Note
TS23: skeletal muscle	Trace		7B	Barely detectable expression in skeletal muscles.