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Gene Expression Data
Assay Details
Assay
Reference: J:3158 Lyons GE, et al., Expression of NCAM isoforms during skeletal myogenesis in the mouse embryo. Dev Dyn. 1992 Jun;194(2):94-104
Assay type: RNA in situ
MGI Accession ID: MGI:5796378
Gene symbol: Ncam1
Gene name: neural cell adhesion molecule 1
Probe: NCAM 180 kDa probe
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen not shown: embryonic day 9.0 (more )
Note: 14 somites
Structure Level Pattern Image Note
TS14: myotome Present Expression first detected in myotomes of 14 somite embryos.

Specimen 1A/B: embryonic day 9.0 (more )
Note: 18 somites
Structure Level Pattern Image Note
TS14: myotome Present 1A/B Expression specifically in myotomal cells which have formed under the dermatome.
TS14: neural tube Absent 1A/B Expression in the neural tube is at or below the level of detection at this stage.

Specimen 2C: embryonic day 9.25 (more )
Note: 21-24 somites
Structure Level Pattern Image Note
TS15: somite Present Scattered 2C Scattered expression over the entire somite.
TS15: neural tube Present Diffuse 2C Diffuse expression in neural tube at a level roughly comparable to that in the somites.
TS15: hindgut Absent 2C Authors report there was no expression in other embryonic structures such as the embryonic hindgut.

Specimen 3A/B: embryonic day 10.5 (more )
Note: ~36 somites
Structure Level Pattern Image Note
TS17: myotome Present Regionally restricted 3A/B Expression in a mature myotome near the forelimb bud and more recently formed caudal myotomes.
TS17: cardiac muscle tissue Absent 3A/B No expression in cardiac myocytes.
TS17: forelimb bud Absent 3A/B No expression in myogenic cells migrating into early limb buds.
TS17: hindlimb bud Absent 3A/B No expression in myogenic cells migrating into early limb buds.
TS17: neural tube Strong Regionally restricted 3A/B High level of expression in the ventral root region of the neural tube.

Specimen 5B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: trunk musculature Weak Regionally restricted 5B Expression in vertebral musculature and intercostal muscles, but at levels lower than those found in the neural tube.
TS20: limb mesenchyme Present Regionally restricted 5B Expression in premuscle masses in the developing limbs.
TS20: spinal cord Present Widespread 5B Isoform is widely expressed in developing spinal cord.
TS20: spinal ganglion Present 5B


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory