Antibody:	8613
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The antibody used in Tables 1 &2 was not detailed; it could have been either #8613 or #6378 MGI:5776190. It was noted that both antibodies gave an identical staining pattern.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		3C
TS20: spinal nerve	Present		3C

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		4E	Staining is associated with cell bodies and fibers.
TS15: central nervous system	Present	Regionally restricted	4E	Staining is associated with cell bodies and fibers in the anteroventral part of the neural tube.

Structure	Level	Pattern	Image	Note
TS17: diencephalon marginal layer	Present		4F
TS17: dorsal root ganglion	Present		4F
TS17: future spinal cord	Present		4F
TS17: midbrain marginal layer	Present		4F
TS17: telencephalon marginal layer	Present		4F
TS17: hindbrain marginal layer	Present		4F	Expression was in the metencephalon and myelencephalon marginal layers.
TS17: trigeminal ganglion	Present		4F
TS17: cranial ganglion	Present	Regionally restricted	4F	All the cranial nerve associated ganglia (geniculate, auditory, superior, submaxillary, petrosal, jugular, nodose) were positive, with the exception of the ciliary ganglion.
TS17: autonomic nervous system	Present	Regionally restricted	4F	Expression was detected in the sympathetic ganglia (sympathetic trunk, superior cervical, celiac, and superior and inferior mesenteric).

Structure	Level	Pattern	Image	Note
TS18: metencephalon marginal layer	Present		5B	Immunoreactive fibers were noted arising from the marginal layer.
TS18: midbrain marginal layer	Present		5B	Immunoreactive fibers were noted arising from the marginal layer.
TS18: metencephalon mantle layer	Absent		5B
TS18: metencephalon ventricular layer	Absent		5B
TS18: midbrain mantle layer	Absent		5B
TS18: midbrain ventricular layer	Absent		5B

Structure	Level	Pattern	Image	Note
TS18: sympathetic ganglion	Present		5D

Structure	Level	Pattern	Image	Note
TS19: myelencephalon marginal layer	Present		6B
TS19: trigeminal ganglion	Present		6B	Immunoreactive fibers were noted growing from the trigeminal ganglion and innervating the eye.

Structure	Level	Pattern	Image	Note
TS19: enteric nervous system	Present		6D

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Present	Scattered	6F	Expression was detected in the olfactory neuroepithelium. Immunoreactive fibers were noted.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present		7D	Expression was detected in the medulla oblongata. Longitudinal and transversal fiber tracts show expression.

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Moderate	Regionally restricted		Ventral horn.
TS15: central nervous system ganglion	Moderate	Regionally restricted		Expression was detected in the geniculate ganglion and the auditory ganglion. There was no expression in the trigeminal ganglion.
TS15: peripheral nervous system	Moderate	Regionally restricted		Expression was detected in the submaxillary ganglion.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Strong	Regionally restricted		Ventral horn.
TS17: dorsal root ganglion	Moderate
TS17: acoustic ganglion	Strong
TS17: facial ganglion	Strong
TS17: trigeminal ganglion	Strong
TS17: cranial ganglion	Moderate	Regionally restricted		Moderate expression was detected in the superior ganglion, petrosal ganglion, jugular ganglion, and the nodose ganglion.
TS17: peripheral nervous system ganglion	Present	Regionally restricted		Expression was detected in the submaxillary ganglion (very strong), the mesenteric ganglion (moderate), and the sup-inf. cervical ganglion (moderate).
TS17: sympathetic nerve trunk	Moderate
TS17: enteric nervous system	Absent			Includes the myenteric, the submucosae plexi and neuronal cells in the wall of the alimentary tract.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Very strong	Regionally restricted		Ventral horn.
TS19: dorsal root ganglion	Very strong
TS19: olfactory epithelium	Moderate	Regionally restricted		Expression was detected in olfactory neuroepithelium.
TS19: retina	Absent
TS19: acoustic ganglion	Very strong
TS19: facial ganglion	Very strong
TS19: superior glossopharyngeal ganglion	Very strong
TS19: trigeminal ganglion	Very strong
TS19: autonomic ganglion	Strong	Regionally restricted		Expression was detected in the submaxillary ganglion, the mesenteric ganglion, and the ganglion cells of the heart.
TS19: inferior glossopharyngeal ganglion	Strong
TS19: inferior vagus ganglion	Strong
TS19: superior vagus ganglion	Strong
TS19: sympathetic nerve trunk	Strong
TS19: enteric nervous system	Strong	Regionally restricted		Includes the myenteric, the submucosae plexi and neuronal cells in the wall of the alimentary tract.
TS19: thoracic ganglion	Moderate	Regionally restricted		Expression was detected in the ganglion cells of the lung.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Very strong	Regionally restricted		Ventral horn.
TS20: olfactory epithelium	Very strong	Regionally restricted		Expression was very strong in olfactory neuroepithelium.
TS20: dorsal root ganglion	Very strong
TS20: retina	Absent
TS20: cervical ganglion	Very strong
TS20: inferior glossopharyngeal ganglion	Very strong
TS20: trigeminal ganglion	Very strong
TS20: submandibular ganglion	Moderate
TS20: sympathetic nerve trunk	Strong
TS20: autonomic ganglion	Very strong	Regionally restricted		Expression was very strong in the mesenteric ganglia.
TS20: enteric nervous system	Very strong			Includes the myenteric, the submucosae plexi and neuronal cells in the wall of the alimentary tract.
TS20: thoracic ganglion	Strong	Regionally restricted		Expression was strong in the ganglion cells of lung.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Very strong	Regionally restricted		Ventral horn.
TS21: olfactory epithelium	Very strong	Regionally restricted		Expression was very strong in olfactory neuroepithelium.
TS21: cochlear ganglion	Very strong
TS21: dorsal root ganglion	Very strong
TS21: facial ganglion	Very strong
TS21: inferior vagus ganglion	Very strong
TS21: superior glossopharyngeal ganglion	Very strong
TS21: superior vagus ganglion	Very strong
TS21: trigeminal ganglion	Very strong
TS21: cranial ganglion	Moderate	Regionally restricted		Expression was detected in ciliary ganglion.
TS21: retina	Strong
TS21: sympathetic nerve trunk	Strong
TS21: autonomic ganglion	Very strong	Regionally restricted		Expression was very strong in mesenteric ganglion.
TS21: enteric nervous system	Very strong			Includes the myenteric, the submucosae plexi and neuronal cells in the wall of the alimentary tract.
TS21: parasympathetic ganglion	Strong	Regionally restricted		Strong expression was detected in the ganglion cells of heart.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Very strong	Regionally restricted		Ventral horn.
TS22: olfactory epithelium	Very strong	Regionally restricted		Expression was very strong in olfactory neuroepithelium.
TS22: cervical ganglion	Very strong
TS22: dorsal root ganglion	Very strong
TS22: inferior glossopharyngeal ganglion	Very strong
TS22: retina	Very strong
TS22: trigeminal ganglion	Very strong
TS22: ciliary ganglion	Strong
TS22: sympathetic nerve trunk	Strong
TS22: submandibular ganglion	Moderate
TS22: autonomic ganglion	Very strong	Regionally restricted		Very strong expression was detected in the mesenteric ganglia.
TS22: thoracic ganglion	Strong	Regionally restricted		Strong expression was detected in the ganglion cells of lung.
TS22: enteric nervous system	Very strong			Includes the myenteric, the submucosae plexi and neuronal cells in the wall of the alimentary tract.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Very strong	Regionally restricted		Ventral horn.
TS23: olfactory epithelium	Very strong	Regionally restricted		Expression was very strong in olfactory neuroepithelium.
TS23: cochlear ganglion	Very strong
TS23: dorsal root ganglion	Very strong
TS23: facial ganglion	Very strong
TS23: inferior glossopharyngeal ganglion	Very strong
TS23: superior glossopharyngeal ganglion	Very strong
TS23: superior vagus ganglion	Very strong
TS23: trigeminal ganglion	Very strong
TS23: submandibular ganglion	Moderate
TS23: sympathetic nerve trunk	Strong
TS23: autonomic ganglion	Very strong	Regionally restricted		Very strong expression was detected in the mesenteric ganglia.
TS23: enteric nervous system	Very strong			Includes the myenteric, the submucosae plexi and neuronal cells in the wall of the alimentary tract.

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent			Expression could not be demonstrated in any presumptive neuronal or non-neuronal structure.

Structure	Level	Pattern	Image	Note
TS14: future telencephalon	Absent			Expression was not detected in the telencephalon primitive vesicle.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Moderate	Regionally restricted		Expression was detected in telencephalon primitive vesicle.
TS15: diencephalon	Absent			There was no expression in the diencephalon primitive vesicle.
TS15: future midbrain	Strong	Regionally restricted		Expression was strong in the ventral mesencephalon primitive vesicle. There was no staining in dorsal mesencephalon primitive vesicle.
TS15: future hindbrain	Strong	Regionally restricted		Expression was strong in the metencephalon primitive vesicle. There was no expression in the myelencephalon primitive vesicle.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Regionally restricted		Expression was detected in telencephalon primitive vesicle.
TS17: diencephalon	Moderate	Regionally restricted		Expression was detected in the diencephalon primitive vesicle. There was no expression in the hypothalamus or thalamus.
TS17: midbrain	Present	Regionally restricted		Expression was very strong in the ventral mesencephalon primitive vesicle and moderate in dorsal mesencephalon primitive vesicle.
TS17: hindbrain	Strong	Regionally restricted		Expression was very strong in the metencephalon primitive vesicle and strong in the myelencephalon primitive vesicle.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted		Expression was detected in telencephalon primitive vesicle (very strong), olfactory bulb (moderate) and basal forebrain (moderate). There was no expression in the neocortex anlage.
TS19: diencephalon	Present	Regionally restricted		Expression was moderate in the diencephalon primitive vesicle and thalamus and strong in the hypothalamus.
TS19: midbrain	Very strong	Regionally restricted		Expression was very strong in the ventral and dorsal mesencephalon primitive vesicle.
TS19: hindbrain	Very strong	Regionally restricted		Expression was very strong in the metencephalon primitive vesicle and in the myelencephalon primitive vesicle.
TS19: metencephalon basal plate	Moderate	Regionally restricted		Expression was detected in the pons.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted		Expression was detected in telencephalon primitive vesicle (very strong), olfactory bulb (strong) and basal forebrain (strong).
TS20: neocortex	Moderate
TS20: diencephalon	Very strong	Regionally restricted		Expression was very strong in the diencephalon primitive vesicle.
TS20: hypothalamus	Strong
TS20: thalamus	Strong
TS20: midbrain	Strong	Regionally restricted		Expression was very strong in the ventral and dorsal mesencephalon primitive vesicle and strong in the tuberculum posterius.
TS20: cerebellum primordium	Moderate
TS20: pons	Strong
TS20: medulla oblongata	Strong	Regionally restricted		Expression was very strong in the metencephalon primitive vesicle and in the myelencephalon primitive vesicle.
TS20: metencephalon	Strong	Regionally restricted		Expression was very strong in the metencephalon primitive vesicle and in the myelencephalon primitive vesicle.

Structure	Level	Pattern	Image	Note
TS21: olfactory lobe	Very strong	Regionally restricted		Expression was very strong in the olfactory bulb.
TS21: neocortex	Strong
TS21: telencephalon	Very strong	Regionally restricted		Expression was very strong in the basal forebrain.
TS21: hypothalamus	Very strong
TS21: thalamus	Very strong
TS21: midbrain	Very strong	Regionally restricted		Expression was very strong in the tuberculum posterius.
TS21: cerebellum	Strong
TS21: medulla oblongata	Strong
TS21: pons	Strong

Structure	Level	Pattern	Image	Note
TS22: basal forebrain	Very strong
TS22: hypothalamus	Very strong
TS22: main olfactory bulb	Very strong
TS22: neocortex	Very strong
TS22: thalamus	Very strong
TS22: midbrain	Very strong	Regionally restricted		Expression was very strong in the tuberculum posterius.
TS22: cerebellum	Very strong
TS22: medulla oblongata	Very strong
TS22: pons	Very strong

Structure	Level	Pattern	Image	Note
TS23: basal forebrain	Very strong
TS23: hypothalamus	Very strong
TS23: main olfactory bulb	Very strong
TS23: neocortex	Very strong
TS23: thalamus	Very strong
TS23: midbrain	Very strong	Regionally restricted		Expression was very strong in the tuberculum posterius.
TS23: cerebellum	Very strong
TS23: medulla oblongata	Very strong
TS23: pons	Very strong