Gene Expression Data
Assay Details
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Assay
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Reference:
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J:138816
Plusa B, et al., Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Development. 2008 Sep;135(18):3081-91
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5776185
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Gene symbol:
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Dab2
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Gene name:
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disabled 2, mitogen-responsive phosphoprotein
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Antibody:
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anti-Dab2
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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Heterozygous and homozygous embryos were indistinguishable.
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Results
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Specimen
1E:
(close  )
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Genetic Background:
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involves: 129S4/SvJaeSor * ICR
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Mutant Allele(s):
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Pdgfratm11(EGFP)Sor/?
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Age:
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embryonic day 4.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Dab2; green - Pdgfra reporter (assay MGI:5776158).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS6: primitive endoderm |
Present |
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1E merge
,
1E DAB2
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Expression is localized to the primitive endoderm layer of the blastocyst; it co-localized with a reporter for another gene that is a marker for this layer.
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Analysis Tools
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