Probe:	HtrA1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The probe reference (Oka et al. 2004) described two probes MGI:5565365 and MGI:5565366; either could have been used in this assay.

Structure	Level	Pattern	Image	Note
TS28: decidua capsularis	Strong		3A , 3B
TS11: ectoplacental cone	Weak	Regionally restricted	3A , 3B	Expression was weak in the outer layer of the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS28: decidua capsularis	Strong		3D , 3H
TS13: ectoplacental cone	Present		3H , 3D	Expression became more prominent in the outer ectoplacental cone at E8.5. Expression was observed in the area where ectoplacental cone markers Tpbpa and Prl2c2 reside.

Structure	Level	Pattern	Image	Note
TS15: spongiotrophoblast	Present	Regionally restricted	3-T , 3-K	Expression was restricted to a thin outer layer of cells in the junctional zone. Cells in this region were also positive for trophoblast lineage markers Tpbpa, Prl2c2 and Prl3b1.
TS15: primary trophoblast giant cell	Absent		3-Q , 3-K

Structure	Level	Pattern	Image	Note
TS17: spongiotrophoblast	Weak		S4A

Structure	Level	Pattern	Image	Note
TS28: decidua basalis	Present	Regionally restricted	S4A	Expression decreased at E10.5, but was still very strong compared to that in the junctional zone.

Structure	Level	Pattern	Image	Note
TS28: decidua basalis	Present	Regionally restricted	S4B , S4C	Expressing cells were located inside the decidua basalis close to the junctional zone. Some expressing cells in the decidua basalis were associated with maternal arteries.

Structure	Level	Pattern	Image	Note
TS28: decidua basalis	Present	Regionally restricted	S4H , S4G	Expressing cells were located inside the decidua basalis close to the junctional zone. Some expressing cells in the decidua basalis were associated with maternal arteries.