Antibody:	Anti-Gpr177 (WLAP-177)
Assay notes:	Two antibodies were described. The N-terminus antibody MGI:5707881 and C-terminus antibody MGI:5707874. The expression patterns were identical between the two antibodies.

Structure	Level	Pattern	Image	Note
TS23: rhombic lip	Present	Regionally restricted	3_left , 3_right	Expression was in the cytoplasm and staining was punctate. Expression was predominately in the interior face of the rhombic lip, while cells of the exterior face of the rhombic lip were negative. The reporter co-localized with antibody staining.

Structure	Level	Pattern	Image	Note
TS19: rhombic lip	Present		4A right , 4A left	There was no co-expression with Atoh1.

Structure	Level	Pattern	Image	Note
TS21: rhombic lip	Present		5A , 5C	Expression was throughout the rhombic lip. There is a column of cells oriented in the anterior to posterior domain that overlaps with Atoh1 expression.

Structure	Level	Pattern	Image	Note
TS23: rhombic lip	Present	Regionally restricted	5D , 5F	Expression was localized primarily to the interior face of the rhombic lip. Expression was largely non-overlapping with Atoh1, except for a few cells. Expression ends abruptly at the presumed dorsal border of the rhombic lip.

Structure	Level	Pattern	Image	Note
TS26: rhombic lip	Present		5I , 5G	There was no overlap of expression with Atoh1.

Structure	Level	Pattern	Image	Note
TS23: rhombic lip	Present	Regionally restricted	6_left , 6_right	Expression was detected in the rhombic lip as in wild type. Co-expression with Atoh1-reporter was seen in a higher number of cells in the interior face of the rhombic lip in the mutant compared to wild type.
TS23: 4th ventricle choroid plexus	Present		6_left , 6_right	Expression was detected as in wild type.
TS23: midbrain-hindbrain junction	Present		6_left , 6_right	Expression was detected as in wild type.

Structure	Level	Pattern	Image	Note
TS23: rhombic lip	Present	Regionally restricted	10A	Expression was detected in cells in the interior face of the rhombic lip, which had fewer BrdU-positive cells than the exterior face of the rhombic lip.