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Gene Expression Data
Assay Details
Assay
Reference: J:216290 Yeung J, et al., Wls provides a new compartmental view of the rhombic lip in mouse cerebellar development. J Neurosci. 2014 Sep 10;34(37):12527-37
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5707890
Gene symbol: Wls
Gene name: wntless WNT ligand secretion mediator
Reporter: lacZ
Antibody: anti-B-galactosidase
Results
Specimen 3: embryonic day 15.5; WlsGt(RRJ545)Byg/Wls+ (more )
Note: Double labeled: green - Wls; red - Wls (assay MGI:5707888).
Structure Level Pattern Image Note
TS23: rhombic lip Present Regionally restricted 3_right , 3_center Expression was in the cytoplasm and staining was punctate. Expression was predominately in the interior face of the rhombic lip, while cells of the exterior face of the rhombic lip were negative. The reporter co-localized with antibody staining.

Specimen 4C: embryonic day 11.5; WlsGt(RRJ545)Byg/Wls+ (more )
Note: Double labeled: green - Wls; red - Lmx1a (assay MGI:5707891).
Structure Level Pattern Image Note
TS19: rhombic lip Present 4C center , 4C right There was co-expression with Lmx1a.
TS19: hindbrain roof plate Present Regionally restricted 4C center , 4C right Expression was detected in the choroid plexus. There was co-expression with Lmx1a.

Specimen 7: embryonic day 15.5; WlsGt(RRJ545)Byg/Wls+ (more )
Note: Double labeled: green - Wls; red - Pax6 (assay MGI:5707893).
Structure Level Pattern Image Note
TS23: rhombic lip Strong Regionally restricted 7_right , 7_left Expression was robust in the interior face of the rhombic lip and light in the exterior face, and strong in the distal part of the rhombic lip. Co-expression was noted with light expressing Pax6-positive cells in the interior face.

Specimen 9A: embryonic day 15.5; WlsGt(RRJ545)Byg/Wls+ (more )
Note: Double labeled: green - Wls; red - Lmx1a (assay MGI:5707891).
Structure Level Pattern Image Note
TS23: rhombic lip Present Regionally restricted 9A right , 9A left Expression was detected in the interior face of the rhombic lip. There was co-expression with Lmx1a, which had weak expression in this region.

Specimen 9B: embryonic day 15.5; WlsGt(RRJ545)Byg/Wls+ (more )
Note: Double labeled: green - Wls; red - Eomes (assay MGI:5707894).
Structure Level Pattern Image Note
TS23: rhombic lip Present Regionally restricted 9B right , 9B left Expression was detected in the interior face of the rhombic lip. There was no co-expression with Tbr2.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory