Gene Expression Data
Assay Details
Assay
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Reference:
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J:193529
Aguado C, et al., Differential maturation of GIRK2-expressing neurons in the mouse cerebellum. J Chem Neuroanat. 2013 Jan;47:79-89
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5707691
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Gene symbol:
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Grm2
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Gene name:
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glutamate receptor, metabotropic 2
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Antibody:
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Anti-mGlu2/3 (AB1553)
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Detection system:
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Secondary antibody
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Assay notes:
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The antibody used in this assay recognizes both Grm2 and Grm3.
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Results
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Specimen
6C:
postnatal adult; Gad1tm1.1Tama/Gad1 +
(more )
Note: Triple labeled: blue - Grm2 and Grm3; red - Kcnj6 (assay MGI:5707612); green - Gad1 reporter (assay MGI:5707688).
Specimen
6C:
(close )
Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Grm2 and Grm3; red - Kcnj6 (assay MGI:5707612); green - Gad1 reporter (assay MGI:5707688).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum granule cell layer |
Present |
Regionally restricted |
6C4
,
6C2
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Expression was detected in Goli cells. Co-expression with Gad1 and Kcnj6 was noted on some cells.
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Specimen
6D:
postnatal adult; Gad1tm1.1Tama/Gad1 +
(more )
Note: Triple labeled: blue - Grm2 and Grm3; red - Kcnj6 (assay MGI:5707612); green - Gad1 reporter (assay MGI:5707688).
Specimen
6D:
(close )
Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
Triple labeled: blue - Grm2 and Grm3; red - Kcnj6 (assay MGI:5707612); green - Gad1 reporter (assay MGI:5707688).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum granule cell layer |
Present |
Regionally restricted |
6D4
,
6D2
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Expression was detected in Goli cells. Some cells were noted that did not co-express Gad1 and Kcnj6.
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