Neo1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:199400 van den Heuvel DM, et al., Spatiotemporal expression of repulsive guidance molecules (RGMs) and their receptor neogenin in the mouse brain. PLoS One. 2013;8(2):e55828
Assay type:	RNA in situ
MGI Accession ID:	MGI:5693243
Gene symbol:	Neo1
Gene name:	neogenin

Probe:	Neo cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1C ©

1C' ©

Specimen 1C: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Weak	Regionally restricted	1C' , 1C	Expression was detected in the apical cell layer.
TS24: ethmoid bone cribriform plate	Weak		1C
TS24: main olfactory bulb mitral cell layer	Weak		1C
TS24: accessory olfactory bulb	Present		1C
TS24: main olfactory bulb ventricular layer	Present		1C

1N ©

1N' ©

Specimen 1N: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Present		1N	Expression was as observed at E16.5.
TS28: main olfactory bulb glomerular layer	Strong	Regionally restricted	1N' , 1N	Strong expression was detected in periglomerular cells.
TS28: main olfactory bulb mitral cell layer	Strong		1N' , 1N
TS28: main olfactory bulb granule cell layer	Strong		1N , 1N'	Expression was strongest in granule cells located adjacent to the inner plexiform layer.

Specimen Table S2 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: olfactory epithelium	Moderate	Regionally restricted	Apical expression.
TS24: ethmoid bone cribriform plate	Moderate
TS24: main olfactory bulb internal plexiform layer	Moderate
TS24: main olfactory bulb prospective granule cell layer	Moderate
TS24: accessory olfactory bulb	Strong
TS24: main olfactory bulb mitral cell layer	Strong
TS24: main olfactory bulb ventricular layer	Very strong
TS24: main olfactory bulb external plexiform layer	Weak
TS24: main olfactory bulb prospective glomerular layer	Weak

Specimen Table S2 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Note
TS28: ethmoid bone cribriform plate	Moderate
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb mitral cell layer	Moderate
TS28: main olfactory bulb ventricular layer	Moderate
TS28: main olfactory bulb external plexiform layer	Weak
TS28: main olfactory bulb internal plexiform layer	Weak
TS28: anterior olfactory nucleus	Strong
TS28: main olfactory bulb granule cell layer	Strong
TS28: olfactory epithelium	Moderate	Regionally restricted	Apical expression.

Specimen Table S2 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Strong
TS28: main olfactory bulb glomerular layer	Strong
TS28: main olfactory bulb external plexiform layer	Weak
TS28: main olfactory bulb internal plexiform layer	Weak
TS28: main olfactory bulb granule cell layer	Moderate
TS28: main olfactory bulb mitral cell layer	Moderate
TS28: main olfactory bulb ventricular layer	Moderate

3C ©

3C' ©

Specimen 3C: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Strong	Widespread	3C' , 3C
TS24: cortical subplate	Present		3C , 3C'
TS24: cortical plate	Present	Regionally restricted	3C' , 3C	Expression was prominent in the upper cortical plate.

4C ©

4C' ©

Specimen 4C: postnatal day 5 (more

)

Structure	Level	Image
TS28: cortical layer I	Present	4C , 4C'
TS28: cortical layer II	Present	4C , 4C'
TS28: cortical layer III	Present	4C , 4C'
TS28: cortical layer IV	Present	4C , 4C'
TS28: cortical layer V	Present	4C , 4C'

Specimen Table S3 E16.5: embryonic day 16.5 (more

)

Structure	Level	Note
TS24: cerebral cortex intermediate zone	Moderate
TS24: cerebral cortex marginal layer	Moderate
TS24: cerebral cortex ventricular layer	Moderate
TS24: telencephalon pia mater	Moderate
TS24: cortical plate	Very strong	Strongest expression in upper part.
TS24: cerebral cortex subventricular zone	Strong
TS24: cortical subplate	Strong

Specimen Table S3 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Strong
TS28: cortical layer II	Strong
TS28: cortical layer III	Strong
TS28: cerebral cortex ventricular zone	Moderate
TS28: cortical layer IV	Moderate
TS28: cortical layer V	Very strong
TS28: cortical layer VI	Absent

Specimen Table S3 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Weak
TS28: cortical layer II	Weak
TS28: cortical layer III	Weak
TS28: cortical layer IV	Weak
TS28: cortical layer V	Weak
TS28: cortical layer VI	Weak
TS28: cerebral cortex ventricular zone	Moderate

6C ©

Specimen 6C: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: dentate gyrus primordium	Strong		6C
TS24: hippocampus	Strong	Regionally restricted	6C	Strong expression was detected in the CA region and the dentate migration stream.
TS24: hippocampus ventricular layer	Weak		6C

6L ©

Specimen 6L: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present	Graded	6L	Expression was detected in a medial high to lateral low gradient.
TS28: hippocampus CA2	Present		6L
TS28: hippocampus CA3	Present		6L
TS28: dentate gyrus granule cell layer	Strong		6L
TS28: dentate gyrus polymorphic layer	Present		6L

Specimen Table S4 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: hippocampus	Very strong	Regionally restricted	Expression was very strong in the CA region. There was weak expression in the subventricular zone.
TS24: dentate gyrus primordium	Very strong
TS24: entorhinal cortex	Very strong
TS24: hippocampus ventricular layer	Moderate

Specimen Table S4 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Strong
TS28: hippocampus CA2	Strong
TS28: hippocampus CA3	Very strong
TS28: dentate gyrus	Strong
TS28: dentate gyrus molecular layer	Moderate
TS28: dentate gyrus granule cell layer	Strong
TS28: entorhinal cortex	Strong
TS28: dentate gyrus polymorphic layer	Moderate
TS28: hippocampus stratum lacunosum	Weak
TS28: hippocampus stratum oriens	Weak
TS28: hippocampus stratum radiatum	Absent

Specimen Table S4 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Moderate
TS28: hippocampus CA2	Moderate
TS28: hippocampus CA3	Moderate
TS28: dentate gyrus granule cell layer	Moderate
TS28: dentate gyrus layer	Moderate
TS28: dentate gyrus polymorphic layer	Moderate
TS28: dentate gyrus molecular layer	Absent
TS28: hippocampus stratum lacunosum	Absent
TS28: hippocampus stratum oriens	Absent
TS28: hippocampus stratum radiatum	Absent
TS28: entorhinal cortex	Strong

8C ©

Specimen 8C: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: medial habenular nucleus	Weak		8C
TS24: lateral habenular nucleus	Weak	Regionally restricted	8C	Weak expression was detected in the medial part of the lateral habenular nucleus.

8F ©

Specimen 8F: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: medial habenular nucleus	Weak		8F
TS24: lateral habenular nucleus	Weak	Regionally restricted	8F	Weak expression was detected in the medial part of the lateral habenular nucleus.

8P ©

Specimen 8P: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: midbrain	Present	Regionally restricted	8P	Expression was detected in a subset of neurons in the ventral tegmental area and substantia nigra.

9C ©

Specimen 9C: postnatal day 5 (more

)

Structure	Level	Image
TS28: lateral habenular nucleus	Weak	9C
TS28: medial habenular nucleus	Weak	9C
TS28: paraventricular thalamic nucleus	Present	9C

9I ©

Specimen 9I: postnatal adult (more

)

Structure	Level	Image
TS28: lateral habenular nucleus	Weak	9I
TS28: medial habenular nucleus	Weak	9I
TS28: paraventricular thalamic nucleus	Present	9I

Specimen Table S5 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: midbrain	Moderate	Regionally restricted	Expression was detected in the substantia nigra and the ventral tegmental area.
TS24: interpeduncular nucleus	Strong
TS24: telencephalon septum	Strong
TS24: thalamus	Strong
TS24: corpus striatum	Moderate
TS24: lateral habenular nucleus	Moderate
TS24: medial habenular nucleus	Moderate
TS24: hypothalamus	Strong	Regionally restricted	Expression was strong in lateral hypothalamus.

Specimen Table S5 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: hypothalamus lateral zone	Moderate
TS28: lateral habenular nucleus	Moderate
TS28: medial habenular nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: ventral tegmental area	Moderate
TS28: corpus striatum	Weak
TS28: interpeduncular nucleus	Weak
TS28: telencephalon septum	Strong
TS28: thalamus	Strong
TS28: paraventricular thalamic nucleus	Very strong

Specimen Table S5 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hypothalamus lateral zone	Weak
TS28: lateral habenular nucleus	Weak
TS28: medial habenular nucleus	Weak
TS28: interpeduncular nucleus	Moderate
TS28: paraventricular thalamic nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: telencephalon septum	Moderate
TS28: thalamus	Moderate
TS28: ventral tegmental area	Moderate
TS28: corpus striatum	Absent

11C ©

Specimen 11C: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: cerebellum Purkinje cell cluster	Present	11C
TS24: cerebellum external granule cell layer	Present	11C
TS24: cerebellum ventricular layer	Present	11C
TS24: deep cerebellar nucleus	Present	11C

11I ©

Specimen 11I: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong	Regionally restricted	11I	Strong expression was detected in the outer part of the EGL.
TS28: cerebellum Purkinje cell layer	Strong		11I
TS28: cerebellum internal granule cell layer	Strong		11I

11O ©

Specimen 11O: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		11O
TS28: cerebellum granule cell layer	Present		11O

Specimen Table S6 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum Purkinje cell cluster	Strong
TS24: cerebellum external granule cell layer	Strong
TS24: cerebellum ventricular layer	Strong
TS24: deep cerebellar nucleus	Strong

Specimen Table S6 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Note
TS28: cerebellum external granule cell layer	Strong	Regionally restricted	Expression was strong in outer EGL layers.
TS28: cerebellum Purkinje cell layer	Strong
TS28: cerebellum internal granule cell layer	Very strong

Specimen Table S6 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong
TS28: cerebellum Purkinje cell layer	Moderate
TS28: cerebellum molecular layer	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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