Probe:	Mkp3 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		2A
TS11: yolk sac	Present		2A

Structure	Level	Pattern	Image	Note
TS12: trunk unsegmented mesenchyme	Present	Regionally restricted	2B	Expressed in the caudal part in two stripes marking primarily the anterior halves of somitomers S-0 and S-I.
TS12: foregut diverticulum	Present		2B
TS12: future prosencephalon	Present		2B
TS12: midbrain-hindbrain junction	Present		2B
TS12: somite	Present		2B
TS12: head mesenchyme	Present	Regionally restricted	2B	Expressed in the region of the forebrain and frontonasal prominence, and near the midbrain/hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	2C	Expressed in the caudal part in two stripes marking primarily the anterior halves of somitomers S-0 and S-I.
TS15: branchial arch mesenchyme	Present		2C
TS15: forelimb bud mesenchyme	Present		2C
TS15: fronto-nasal process	Present		2C
TS15: future forebrain	Present		2C
TS15: midbrain-hindbrain junction	Present		2C
TS15: somite	Present		2C
TS15: neural plate	Present	Regionally restricted	2C	Expressed in the caudal end.
TS15: tail	Present	Regionally restricted	2C	Expressed in the region of the primitive streak and tail bud.
TS15: head mesenchyme	Present	Regionally restricted	2C	Expressed in the region of the forebrain and frontonasal prominence, and near the midbrain/hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	2D	Expressed in the caudal part in two stripes marking primarily the anterior halves of somitomers S-0 and S-I.
TS17: forebrain	Present		2D
TS17: fronto-nasal process	Present		2D
TS17: hindlimb bud mesenchyme	Present		2D
TS17: midbrain-hindbrain junction	Present		2D
TS17: somite	Present		2D
TS17: neural tube	Present	Regionally restricted	2D	Expressed in the caudal end.
TS17: tail	Present	Regionally restricted	2D	Expressed in the region of the primitive streak and tail bud.
TS17: head mesenchyme	Present	Regionally restricted	2D	Expressed in the region of the forebrain and frontonasal prominence, and near the midbrain/hindbrain boundary.
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	2D	Expressed in the tooth anlagen.
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	2D	Expressed in the tooth anlagen.
TS17: 1st branchial arch	Present	Regionally restricted	2D	Expressed in a small region close to the cleft.
TS17: 2nd branchial arch	Present	Regionally restricted	2D	Expressed in a small region close to the cleft.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	2E	Expressed in the caudal part in two stripes marking primarily the anterior halves of somitomers S-0 and S-I.
TS19: forebrain	Present		2E
TS19: fronto-nasal process	Present		2E
TS19: somite	Present		2E
TS19: neural tube	Present	Regionally restricted	2E	Expressed in the caudal end.
TS19: tail	Present	Regionally restricted	2E	Expressed in the region of the primitive streak and tail bud.
TS19: head mesenchyme	Present	Regionally restricted	2E	Expressed in the region of the forebrain and frontonasal prominence.
TS19: 1st branchial arch	Present	Regionally restricted	2E	Expressed in the tooth anlagen and a small region close to the cleft.
TS19: 2nd branchial arch	Present	Regionally restricted	2E	Expressed in a small region close to the cleft.
TS19: forelimb bud mesenchyme	Present	Regionally restricted	2E	Expressed in the progress zone adjacent to the apical ectodermal ridge. Weaker expression is detected in the central proximal mesenchyme.
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	2E	Expressed in the progress zone adjacent to the apical ectodermal ridge. Weaker expression is detected in the central proximal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	2F	Expressed in the region of the forebrain and frontonasal prominence.
TS20: forebrain	Present		2F
TS20: mammary bud	Present		2F
TS20: nose	Present		2F
TS20: vibrissa follicle	Present		2F
TS20: forelimb mesenchyme	Present	Regionally restricted	2F	Expressed in the progress zone adjacent to the apical ectodermal ridge. Weaker expression is detected in the central proximal mesenchyme.
TS20: hindlimb mesenchyme	Present	Regionally restricted	2F	Expressed in the progress zone adjacent to the apical ectodermal ridge. Weaker expression is detected in the central proximal mesenchyme.
TS20: tail	Present	Regionally restricted	2F	Expressed in the tail bud.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present		2G
TS21: hair follicle	Present		2G
TS21: hindlimb	Present		2G
TS21: pinna	Present		2G
TS21: vibrissa follicle	Present		2G

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	2H	Expressed in the caudal part in two stripes marking primarily the anterior halves of somitomers S-0 and S-I.
TS15: somite	Present		2H
TS15: neural plate	Present	Regionally restricted	2H	Expressed in the caudal end.
TS15: tail	Present	Regionally restricted	2H	Expressed in the region of the primitive streak and tail bud.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	2I	Expressed in the caudal part in two stripes marking primarily the anterior halves of somitomers S-0 and S-I.
TS19: somite	Present		2I
TS19: neural tube	Present	Regionally restricted	2I	Expressed in the caudal end.
TS19: tail	Present	Regionally restricted	2I	Expressed in the region of the primitive streak and tail bud.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	2J	Expressed in the caudal part in two stripes marking primarily the anterior halves of somitomers S-0 and S-I.
TS19: somite	Present		2J
TS19: neural tube	Present	Regionally restricted	2J	Expressed in the caudal end.
TS19: tail	Present	Regionally restricted	2J	Expressed in the region of the primitive streak and tail bud.

Structure	Level	Pattern	Image	Note
TS20: tail unsegmented mesenchyme	Present	Regionally restricted	2K	Expressed in the caudal part in two stripes marking primarily the anterior halves of somitomers S-0 and S-I.
TS20: somite	Present		2K
TS20: spinal cord	Present	Regionally restricted	2K	Expressed in the caudal end.
TS20: tail	Present	Regionally restricted	2K	Expressed in the region of the primitive streak and tail bud.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present		2L

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present		2M

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Present	Regionally restricted	2N	Expressed in the progress zone adjacent to the apical ectodermal ridge. Weaker expression is detected in the central proximal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present	Regionally restricted	2O	Expressed in the progress zone adjacent to the apical ectodermal ridge. Weaker expression is detected in the central proximal mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: digit mesenchyme	Present	Regionally restricted	2P	Expressed around the digit tips.

Structure	Level	Pattern	Image	Note
TS15: dermomyotome	Present		3A
TS15: myotome	Present		3A
TS15: sclerotome	Weak		3A

Structure	Level	Pattern	Image	Note
TS19: dermomyotome	Present		3B
TS19: myotome	Present		3B
TS19: sclerotome	Weak		3B

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	3C	Expressed in the tooth anlagen.
TS17: hindlimb bud mesenchyme	Present		3C

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present		3D
TS22: submandibular gland primordium	Present		3D
TS22: tooth	Present		3D
TS22: ureteric tip	Present		3D
TS22: lung	Weak		3D

Structure	Level	Pattern	Image	Note
TS22: vibrissa follicle	Present		3E

Structure	Level	Pattern	Image	Note
TS22: tooth	Present		3F
TS22: vibrissa follicle	Present		3F

Structure	Level	Pattern	Image	Note
TS22: ureteric tip	Present		3G

Structure	Level	Pattern	Image	Note
TS23: tooth	Present		3H
TS23: vibrissa follicle	Present		3H