Probe:	P450RA probe2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS8: embryo endoderm	Present
TS8: extraembryonic endoderm	Present
TS8: epiblast	Absent
TS8: extraembryonic ectoderm	Absent

Structure	Level	Pattern	Image	Note
TS9: embryo endoderm	Present		7A
TS9: embryo mesoderm	Present		7A
TS9: extraembryonic ectoderm	Present		7A
TS9: extraembryonic endoderm	Present		7A
TS9: embryo ectoderm	Absent		7A

Structure	Level	Pattern	Image	Note
TS10: embryo endoderm	Present		7B
TS10: extraembryonic ectoderm	Present		7B
TS10: extraembryonic endoderm	Present		7B
TS10: extraembryonic mesoderm	Present		7B
TS10: embryo mesoderm	Strong		7B	Expression in mesoderm and primitive streak (excluding the node) becomes more evident.
TS10: primitive streak	Strong		7B	Expression in mesoderm and primitive streak (excluding the node) becomes more evident.
TS10: embryo ectoderm	Absent		7B
TS10: node	Absent		7B

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		7C	Expression in anterior region of all three embryonic germ layers.
TS11: embryo endoderm	Present		7C	Expression in anterior region of all three embryonic germ layers.
TS11: embryo mesoderm	Weak	Regionally restricted	7C	Expression in anterior region of all three embryonic germ layers. Expression in posterior mesoderm and primitive streak has become weaker.
TS11: primitive streak	Weak	Regionally restricted	7C	Expression in anterior region of all three embryonic germ layers. Expression in posterior mesoderm and primitive streak has become weaker.
TS11: node	Absent		7C
TS11: extraembryonic endoderm	Present		7C	In the extraembryonic region, expression only in endoderm.
TS11: extraembryonic ectoderm	Absent		7C
TS11: extraembryonic mesoderm	Absent		7C

Structure	Level	Pattern	Image	Note
TS12: rhombomere	Present	Regionally restricted		Expression in cells located in the ventrolateral region of rhombomere 2.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	7D	Expression in an anterior region. High expression in a posterior region.
TS13: 1st branchial arch	Present		7D
TS13: foregut diverticulum endoderm	Present		7D
TS13: rhombomere 2	Present		7D
TS13: future spinal cord neural plate	Strong		7D	High expression in a posterior region including the caudal neural plate and hindgut endoderm.
TS13: hindgut diverticulum endoderm	Strong		7D	High expression in a posterior region including the caudal neural plate and hindgut endoderm.

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Absent		7E
TS9: embryo mesoderm	Present		7E
TS9: primitive streak	Present		7E

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Absent		7F
TS10: embryo mesoderm	Strong	Graded	7F	Expression in embryonic mesoderm becomes more evident, with a postero-anterior gradient in mesodermal expression.
TS10: primitive streak	Strong		7F	Expression in primitive streak becomes more evident.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	7G	Expression in anterior region of all three embryonic germ layers.
TS11: embryo mesoderm	Weak	Regionally restricted	7G	Expression in posterior mesoderm has become weaker
TS11: primitive streak	Weak		7G	Expression in primitive streak has become weaker

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Present		7H
TS13: foregut diverticulum endoderm	Present		7H
TS13: rhombomere 2	Present		7H
TS13: migrating neural crest	Present	Regionally restricted	7H

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	7I	Expression in cells in mesenchyme between first branchial arch and hindbrain, which authors state are probably migrating neural crest cells. Expression in neural crest cells for cranial ganglia.
TS15: tail mesenchyme	Present		7I
TS15: tail neural plate	Present		7I
TS15: forelimb bud	Weak	Uniform	7I	Weak expression evenly detected in whole limb buds.
TS15: eye	Weak		7I
TS15: otocyst	Weak		7I
TS15: oral region	Weak	Regionally restricted	7I	Weak expression in maxillo-mandibular cleft.

Structure	Level	Pattern	Image	Note
TS17: cranial ganglion	Present	Regionally restricted	7J	Expression in (anterior) Region 2 including neural crest cells for cranial ganglia still evident
TS17: tail neural tube	Present		7J	Expression in (posterior) Region 1 detected at only at tail end.
TS17: forelimb bud	Weak	Uniform	7J	Weak expression evenly detected in whole limb buds.
TS17: hindlimb bud	Weak	Uniform	7J	Weak expression evenly detected in whole limb buds.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	7K	No expression in both (posterior) Region 1 or (anterior) Region 2.
TS19: eye	Weak		7K
TS19: inner ear	Weak		7K
TS19: forelimb bud	Weak	Regionally restricted	7K	Weak expression in forelimb bud and hindlimb bud, restricted to interdigital space.
TS19: hindlimb bud	Weak	Regionally restricted	7K	Weak expression in forelimb bud and hindlimb bud, restricted to interdigital space.
TS19: oral region	Weak	Regionally restricted	7K	Weak expression maxillo-mandibular cleft.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present	Regionally restricted	7L	Expression in the dorsoventral boundary region of the neural retina.

Structure	Level	Pattern	Image	Note
TS19: handplate	Present	Regionally restricted	7M	Expression restricted to interdigital space.
TS19: forelimb bud	Present	Regionally restricted	7M	Expression also detected in a base region of the forelimb bud as a sharp line.

Structure	Level	Pattern	Image	Note
TS19: footplate	Present	Regionally restricted	7N	Expression restricted to interdigital space.
TS19: hindlimb bud	Present	Regionally restricted	7N	No expressing region at the base of the hindlimb bud.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		8A

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	8C	Expression in anterior (Region 2) in clusters of neural crest cells for cranial ganglia, V, VII/VIII and IX/X.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		8E

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present		8G
TS15: tail mesenchyme	Present		8G
TS15: tail neural plate	Present		8G

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	8I	Expression in the ventrolateral region of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	8K	Expression in the ventrolateral region of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	8M	Expression in the ventrolateral region of the otic vesicle.