Antibody:	anti-OTX2
Assay notes:	Two antibodies were described MGI:1309322 and MGI:5662473; the one used in this assay was not specified.

Structure	Level	Pattern	Image	Note
TS8: anterior visceral endoderm	Present		3J
TS8: epiblast	Present		3J

Structure	Level	Pattern	Image	Note
TS8: epiblast	Weak		3J'
TS8: anterior visceral endoderm	Weak	Regionally restricted	3J'	Some cells showed a reduction of expression in the mutant. Expression of the visceral endoderm marker Foxa2 was normal in these cells.

Structure	Level	Pattern	Image	Note
TS8: anterior visceral endoderm	Present		3K
TS8: epiblast	Present		3K

Structure	Level	Pattern	Image	Note
TS8: epiblast	Weak		3K'
TS8: anterior visceral endoderm	Weak	Regionally restricted	3K'	Some cells showed a reduction of expression in the mutant.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	3M	Expression was detected in anterior neuroectoderm.
TS11: mesendoderm	Present	Regionally restricted	3M	Expression was detected in axial mesendoderm.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	3M'	Some cells in the anterior neural ectoderm showed a reduction of expression in the mutant.
TS11: mesendoderm	Present	Regionally restricted	3M'	Some cells in the axial mesendoderm showed a reduction of expression in the mutant.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	3N	Expression was detected in anterior neuroectoderm.
TS11: mesendoderm	Present	Regionally restricted	3N	Expression was detected in axial mesendoderm.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Weak	Regionally restricted	3N'	Some cells in the anterior neural ectoderm showed a reduction of expression in the mutant.
TS11: mesendoderm	Weak	Regionally restricted	3N'	Some cells in the axial mesendoderm showed a reduction of expression in the mutant.

Structure	Level	Pattern	Image	Note
TS8: primitive endoderm	Present		6G

Structure	Level	Pattern	Image	Note
TS8: primitive endoderm	Present		6H

Structure	Level	Pattern	Image	Note
TS8: primitive endoderm	Present	Single cells	6K	Only a few visceral endoderm cells retained immunostaining in the mutant compared to wild type.

Structure	Level	Pattern	Image	Note
TS8: primitive endoderm	Present	Single cells	6L	Only a few visceral endoderm cells retained immunostaining in the mutant compared to wild type.

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	7M , S5B	Expression was detected in axial mesendoderm.
TS11: neural ectoderm	Present	Regionally restricted	S5B , 7M	Expression was detected in anterior neural ectoderm.

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	7N , S5C	Expression was detected in axial mesendoderm.
TS11: neural ectoderm	Present	Regionally restricted	7N , S5C	Expression was detected in anterior neural ectoderm.

Structure	Level	Pattern	Image	Note
TS11: primitive endoderm	Present		7R , S5J
TS11: neural ectoderm	Present		S5J , 7R

Structure	Level	Pattern	Image	Note
TS11: primitive endoderm	Present		7S , S5K
TS11: neural ectoderm	Present		S5K , 7S

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present		S5F

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present		S5G