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Gene Expression Data
Assay Details
Assay
Reference: J:153562 Acampora D, et al., Selective inactivation of Otx2 mRNA isoforms reveals isoform-specific requirement for visceral endoderm anteriorization and head morphogenesis and highlights cell diversity in the visceral endoderm. Mech Dev. 2009 Oct;126(10):882-97
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5688200
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Antibody: Anti-T
Results
Specimen 3K: embryonic day 6.5 (more )
Note: Double labeled: red - T; green - Otx2 (assay MGI:5688185).
Structure Level Pattern Image Note
TS8: embryo Present Regionally restricted 3K Expression was detected in the early primitive streak.
Specimen 3K': embryonic day 6.5; Otx2tm9Asim/Otx2tm11Asim (more )
Note: Double labeled: red - T; green - Otx2 (assay MGI:5688185).
Structure Level Pattern Image Note
TS8: embryo Present Regionally restricted 3K' Expression in the early primitive streak was normal in the mutant.
Specimen 3O: embryonic day 7.5 (more )
Note: Double labeled: red - T; green - Lhx1 (assay MGI:5688189).
Structure Level Pattern Image Note
TS11: primitive streak Present 3O
Specimen 3O': embryonic day 7.5; Otx2tm9Asim/Otx2tm11Asim (more )
Note: Double labeled: red - T; green - Lhx1 (assay MGI:5688189).
Structure Level Pattern Image Note
TS11: primitive streak Present 3O' Expression was normal in the mutant.
Specimen 6H: embryonic day 6.5 (more )
Note: Double labeled: red - T; green - Otx2 (assay MGI:5688185).
Structure Level Pattern Image Note
TS8: embryo Present Regionally restricted 6H Expression was detected in the early primitive streak.
Specimen 6L: embryonic day 6.5; Otx2tm10Asim/Otx2tm11Asim (more )
Note: Double labeled: red - T; green - Otx2 (assay MGI:5688185).
Structure Level Pattern Image Note
TS8: embryo Present Regionally restricted 6L Expression in the Otx2<deltaCD/GFP> and Otx2<GFP/GFP> mutants was similar.
Specimen 6O: embryonic day 6.5; Otx2tm11Asim/Otx2tm11Asim (more )
Note: Double labeled: red - T; green - Lhx1 (assay MGI:5688189).
Structure Level Pattern Image Note
TS8: embryo Present Regionally restricted 6O Expression in the Otx2<deltaCD/GFP> and Otx2<GFP/GFP> mutants was similar.
Specimen 7O: embryonic day 7.5 (more )
Note: Double labeled: red - T; green - Lhx1 (assay MGI:5688189).
Structure Level Pattern Image Note
TS11: mesendoderm Present Regionally restricted S5D , 7O Expression was detected in axial mesendoderm in head process cells.
Specimen S6A: embryonic day 7.5; Otx2tm1Pas/Otx2+ (more )
Note: Double labeled: red - T; green - Otx2 reporter (assay MGI:5688750).
Structure Level Pattern Image Note
TS11: mesendoderm Present Regionally restricted S6A Expression was detected in axial mesendoderm cells fated to form the head process.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory