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Gene Expression Data
Assay Details
Assay
Reference: J:90459 Johnson CL, et al., Mist1 is necessary for the establishment of granule organization in serous exocrine cells of the gastrointestinal tract. Mech Dev. 2004 Mar;121(3):261-72
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5659913
Gene symbol: Amy
Gene name: amylase complex
Antibody: anti-amylase
Detection system: Secondary antibody coupled to rhodamine
Results
Specimen 2A: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: pancreatic acinus Present 2A Expression is limited to the apical portion of the cells.
Specimen 2B: embryonic day 16.5; Bhlha15tm1Skz/Bhlha15tm1Skz (more )
Structure Level Pattern Image Note
TS24: pancreatic acinus Weak 2B The protein is not confined to the luminal border in the mutants; there are few presumptive zymogen granules.
Specimen 2C: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: pancreatic acinus Present 2C Expression is limited to the apical portion of the cells.
Specimen 2D: embryonic day 16.5; Bhlha15tm1Skz/Bhlha15tm1Skz (more )
Structure Level Pattern Image Note
TS24: pancreatic acinus Weak 2D The protein is not confined to the luminal border in the mutants; there are few presumptive zymogen granules.
Specimen 2E: embryonic day 19.5; Bhlha15tm1Skz/Bhlha15tm1Skz (more )
Structure Level Pattern Image Note
TS26: pancreatic acinus Present 2E Expression is present in nuclei.
Specimen 2F: embryonic day 19.5; Bhlha15tm1Skz/Bhlha15tm1Skz (more )
Structure Level Pattern Image Note
TS26: pancreatic acinus Present 2F Expression is present in nuclei.
Specimen 4D: embryonic day 19.5 (more )
Note: Double labeled: red - amylase; green - Cx32/Gjb1 (assay MGI:5659918).
Structure Level Pattern Image Note
TS26: pancreatic acinus Present 4D
Specimen 4E: embryonic day 19.5 (more )
Note: Double labeled: red - amylase; green - Cx32/Gjb1 (assay MGI:5659918).
Structure Level Pattern Image Note
TS26: pancreatic acinus Present 4E
Specimen 4F: embryonic day 19.5 (more )
Structure Level Pattern Image Note
TS26: pancreatic acinus Present 4F Expression is restricted to the luminal areas of the cells.
Specimen 4G: embryonic day 19.5; Bhlha15tm1Skz/Bhlha15tm1Skz (more )
Structure Level Pattern Image Note
TS26: pancreatic acinus Present 4G Expression is not localized in the mutant, unlike in wild type where it is restricted to the luminal areas of the cells.
Specimen 4H: embryonic day 19.5; Gjb1tm1Kwi/Gjb1tm1Kwi (more )
Structure Level Pattern Image Note
TS26: pancreatic acinus Present 4H As in wild type, expression is restricted to the luminal areas of the cells.
Specimen not shown: postnatal; Bhlha15tm1Skz/Bhlha15tm1Skz (more )
Structure Level Pattern Image Note
TS28: parotid gland Present The parotid tissue is disorganized in the mutant; no typical acini are observed and zymogen granules extend throughout the cells.
Specimen 8D: postnatal adult; Bhlha15tm1Skz/Bhlha15tm1Skz (more )
Structure Level Pattern Image Note
TS28: pancreatic acinus Present 8D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory