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Gene Expression Data
Assay Details
Assay
Reference: J:154785 Arnold SJ, et al., Generation and analysis of a mouse line harboring GFP in the Eomes/Tbr2 locus. Genesis. 2009 Nov;47(11):775-81
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5646122
Gene symbol: Eomes
Gene name: eomesodermin
Reporter: GFP
Direct Detection
Results
Specimen 2a: embryonic day 5.5; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS7: extraembryonic ectoderm Present 2a
TS7: anterior visceral endoderm Present 2a Expressed at a lower levels compared with that of the extraembryonic ectoderm.
TS7: epiblast Absent 2a
Specimen 2c: embryonic day 6.5; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS9: anterior visceral endoderm Present 2c
TS9: definitive endoderm Present 2c
TS9: primitive streak Present 2c
Specimen 2d: embryonic day 7.5; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS11: primitive streak Present Regionally restricted 2d Predominantly in the region of the anterior.
TS11: definitive endoderm Present Regionally restricted 2d Expressed in the region of the anterior.
TS11: chorion Ambiguous 2d No or only very weak expression.
TS11: ectoplacental cone Ambiguous 2d No or only very weak expression.
Specimen 3b: embryonic day 7.5; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS11: primitive streak Present Widespread 3b Expressed throughout the primitive streak region.
TS11: anterior visceral endoderm Present Single cells 3b Expressed in a few cells.
TS11: extraembryonic ectoderm Absent 3b
Specimen 3c: embryonic day 7.5; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS11: primitive streak Present Widespread 3c Expressed throughout the primitive streak region.
TS11: anterior visceral endoderm Present Single cells 3c Expressed in a few cells.
TS11: extraembryonic ectoderm Absent 3c
Specimen 4c/d: embryonic day 12.0; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS20: cortical preplate Present 4c , 4d Expression extends more lateral than immunofluorescent staining.
Specimen 4c'/d': embryonic day 12.0; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS20: cortical preplate Present 4d' , 4c' Expression extends more lateral than immunofluorescent staining.
Specimen 4g/h: embryonic day 15.0; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present 4h , 4g
TS23: cerebral cortex ventricular layer Present 4h , 4g
Specimen 4g'/h': embryonic day 15.0; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present 4g' , 4h'
TS23: cerebral cortex subventricular zone Present 4h' , 4g' Expression extends marginally more toward the intermediate zone than immunofluorescent staining.
Specimen 5a: postnatal day 50; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS28: main olfactory bulb glomerular layer Strong 5a right
TS28: main olfactory bulb mitral cell layer Strong 5a right
Specimen 5b: postnatal day 50; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS28: main olfactory bulb subependymal zone Present Regionally restricted 5b right Expressed in some cells adjacent to the ventricles.
Specimen 5c: postnatal day 50; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS28: telencephalon septum Present Regionally restricted 5c right Expressed in ventral septum.
Specimen 5d: postnatal day 50; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS28: cerebellum internal granule cell layer Weak 5d right The colocalization with endogenous protein was not as obvious compared to other locations.
Specimen 5e: postnatal day 50; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Present Clusters 5e right Overlaped with endogenous protein expression to a significantly higher degree.
Specimen not shown: postnatal day 50; Eomestm2.1Rob/Eomes+ (more )
Structure Level Pattern Image Note
TS28: nucleus of lateral olfactory tract Strong
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory