About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:178190 Sanematsu F, et al., DOCK180 is a Rac activator that regulates cardiovascular development by acting downstream of CXCR4. Circ Res. 2010 Oct 29;107(9):1102-5
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5635647
Gene symbol: Cxcr4
Gene name: C-X-C motif chemokine receptor 4
Antibody: Anti-Cxcr4 (2B11)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488
Results
Specimen Online VC WT: embryonic day 12.5 (more )
Note: Zinc fixative was used. Double labeled: green - Cxcr4; red - Pecam1 (assay MGI:5635649).
Structure Level Pattern Image Note
TS20: midgut Present Online VC WT Cxcr4 , Online VC WT Merge
Specimen Online VC 180d/d: embryonic day 12.5; Dock1tm1.1Ysfk/Dock1tm1.1Ysfk (more )
Note: Zinc fixative was used. Double labeled: green - Cxcr4; red - Pecam1 (assay MGI:5635649).
Structure Level Pattern Image Note
TS20: midgut Present Online VC 180d/d Cxcr4 , Online VC 180d/d Merge
Specimen Online VC CXCR4-/-: embryonic day 12.5; Cxcr4tm1Tng/Cxcr4tm1Tng (more )
Note: Zinc fixative was used. Double labeled: green - Cxcr4; red - Pecam1 (assay MGI:5635649).
Structure Level Pattern Image Note
TS20: midgut Absent Online VC Cxcr4-/- Merge , Online VC Cxcr4-/- Cxcr4
Specimen Online VIA: embryonic day 12.5 (more )
Note: Zinc fixative was used. Double labeled: green - Cxcr4; red - Dock1 (assay MGI:5635660).
Structure Level Pattern Image Note
TS20: midgut vascular element Present Online VIA Merge , Online VIA Cxcr4 There was co-localization of expression with Dock1 in the vascular endothelial cell lineage.
Specimen Online VIB: embryonic day 12.5 (more )
Note: Zinc fixative was used. Double labeled: green - Cxcr4; red - Dock1 (assay MGI:5635660).
Structure Level Pattern Image Note
TS20: endocardial cushion Present Online VIB Cxcr4 , Online VIB Merge There was co-localization of expression with Dock1 in the cardiac cell lineage.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory