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Gene Expression Data
Assay Details
Assay
Reference: J:167031 Petkau TL, et al., Progranulin expression in the developing and adult murine brain. J Comp Neurol. 2010 Oct 1;518(19):3931-47
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5620507
Gene symbol: Grn
Gene name: granulin
Antibody: Anti-Progranulin (AF2557)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: Two different mouse strains were used - C57BL/6 and 129Sv/ImJ. No strain differences were detected and results from both strains were pooled. Individual specimen strain backgrounds were not provided except in the case of data from Table 2, which is indicated below.
Results
Specimen 2A,B: embryonic day 15.5 (more )
Note: Double labeled: red - Grn; green - Pcna (assay MGI:5620510).
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present 2B , 2A There was no co-label with Pcna in proliferating cells in the ventricular zone.

Specimen 2C: embryonic day 15.5 (more )
Note: Double labeled: red - Grn; green - Nes (assay MGI:5620511).
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present 2C There was no co-label with Nestin in immature neuronal precursors in the ventricular zone.

Specimen 2D: embryonic day 15.5 (more )
Note: Double labeled: red - Grn; green - Dcx (assay MGI:5620512).
Structure Level Pattern Image Note
TS23: cerebral cortex intermediate zone Present 2D There was no co-label with Doublecortin in postmitotic, migrating immature neurons.

Specimen 2E: embryonic day 15.5 (more )
Note: Double labeled: red - Grn; green - Rbfox3 (assay MGI:5620513).
Structure Level Pattern Image Note
TS23: piriform cortex Present 2E There was no co-label with NeuN in layer VI of the piriform cortex.

Specimen 2F: postnatal day 0 (more )
Note: Double labeled: red - Grn; green - Rbfox3 (assay MGI:5620513).
Structure Level Pattern Image Note
TS27: piriform cortex Present 2F Expression was stronger and more distinct in postmitotic neurons at P0, and staining was detected in NeuN-positive mature neurons.

Specimen 3A,C: postnatal day 7 (more )
Note: Double labeled: red - Grn; green - Aif1 (assay MGI:5620613).
Structure Level Pattern Image Note
TS28: cerebellum white matter Present Scattered 3A , 3C There was co-localization with Aif1 in microglia.

Specimen 3D: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: hindbrain Present Scattered 3D Expression was detected in microglia in the region of the developing cerebellum.

Specimen 3E: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: cerebellum white matter Present 3E Expression was detected in microglia migrating through the white matter into the cerebellum.

Specimen 3F: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: cerebellum white matter Present 3F Expression was detected in microglia.

Specimen not shown P14: postnatal day 14 (more )
Structure Level Pattern Image Note
TS28: cerebellum white matter Present Expression was detected in microglia.
TS28: corpus callosum Present Expression was detected in microglia.

Specimen not shown P0: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: corpus callosum Present Regionally restricted Expression was detected in microglia.

Specimen 3G: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: corpus callosum Present 3G Expression was detected in microglia.

Specimen 3H: postnatal month 3-4 (more )
Structure Level Pattern Image Note
TS28: corpus callosum Absent 3H There was a nearly complete lack of immunopositive microglia in the corpus callosum.
TS28: cerebral cortex Present Regionally restricted 3H Expression was detected in cortical neurons surrounding the corpus callosum.

Specimen 3I: postnatal month 3-4 (more )
Structure Level Pattern Image Note
TS28: cerebellum white matter Absent 3I

Specimen 5B,C: postnatal month 3-4 (more )
Note: Double labeled: red - Grn; green - Rbfox3 (assay MGI:5620513).
Structure Level Pattern Image Note
TS28: cerebral cortex Present 5C , 5B Expression co-localized with NeuN-labeled neurons.

Specimen 5E,F: postnatal month 3-4 (more )
Note: Double labeled: red - Grn; green - Aif1 (assay MGI:5620613).
Structure Level Pattern Image Note
TS28: cerebral cortex Present 5E , 5F Expression co-localized with Aif1-labeled microglia.

Specimen 5H,I: postnatal month 3-4 (more )
Note: Double labeled: red - Grn; green - Gfap (assay MGI:5620633).
Structure Level Pattern Image Note
TS28: cerebral cortex Present 5I , 5H Expression did not co-localized with Gfap-labeled astrocytes.

Specimen S1I,J: postnatal day 7; Grntm1Blrl/Grn+ (more )
Note: Double labeled: red - Grn; green - Grn reporter (assay MGI:5620499).
Structure Level Pattern Image Note
TS28: cerebellum Present S1I , S1J Co-labeling with the knock-in reporter indicates that the antibody and the knock-in reporter label the identical set of cells.

Specimen Table 2: postnatal month 3-4 (more )
Structure Level Pattern Image Note
TS28: cingulate cortex Strong
TS28: hippocampus CA3 Strong
TS28: hypothalamus Strong
TS28: midbrain reticular formation Strong
TS28: prelimbic area Strong
TS28: primary motor cortex Strong
TS28: thalamus Strong
TS28: visual cortex Strong
TS28: corpus striatum Weak
TS28: dentate gyrus Weak
TS28: lateral septal complex Weak
TS28: medulla oblongata Weak
TS28: pons Weak
TS28: cerebellum Purkinje cell layer Moderate
TS28: hippocampus CA1 Moderate
TS28: subiculum Moderate
TS28: substantia nigra Moderate
TS28: superior colliculus Moderate
TS28: cerebellum granule cell layer Absent
TS28: cerebellum molecular layer Absent


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory