Gene Expression Data
Assay Details
Assay
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Reference:
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J:167031
Petkau TL, et al., Progranulin expression in the developing and adult murine brain. J Comp Neurol. 2010 Oct 1;518(19):3931-47
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5620499
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Gene symbol:
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Grn
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Gene name:
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granulin
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative: 0.2% glutaraldehyde and 2% paraformaldehyde.
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Results
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Specimen
1A,B:
embryonic day 15.5; Grntm1Blrl/Grn +
(more )
Specimen
1A,B:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
1C:
embryonic day 15.5; Grntm1Blrl/Grn +
(more )
Specimen
1C:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
1D:
postnatal day 0; Grntm1Blrl/Grn +
(more )
Specimen
1D:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS27: neocortex |
Weak |
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1D
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Numerous cells were labeled, but the intensity of the staining was weak in any given cell. Staining in neurons became initially apparent.
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Specimen
1E:
postnatal day 7; Grntm1Blrl/Grn +
(more )
Specimen
1E:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
1F:
postnatal month 3-4; Grntm1Blrl/Grn +
(more )
Specimen
1F:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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postnatal month 3-4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
4B:
postnatal month 3-4; Grntm1Blrl/Grn +
(more )
Specimen
4B:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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postnatal month 3-4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: neocortex layer |
Present |
Non-Uniform |
4B
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Expression was detected in all cell layers of the neocortex. Staining was more intense in the large pyramidal neurons of layers V and VI.
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TS28: cortical layer V |
Strong |
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4B
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Staining was more intense in the large pyramidal neurons.
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TS28: cortical layer VI |
Strong |
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4B
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Staining was more intense in the large pyramidal neurons.
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Specimen
4D:
postnatal month 3-4; Grntm1Blrl/Grn +
(more )
Specimen
4D:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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postnatal month 3-4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
not shown:
postnatal month 3-4; Grntm1Blrl/Grn +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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postnatal month 3-4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
|
Specimen
4F:
postnatal month 3-4; Grntm1Blrl/Grn +
(more )
Specimen
4F:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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postnatal month 3-4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
4H:
postnatal month 3-4; Grntm1Blrl/Grn +
(more )
Specimen
4H:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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postnatal month 3-4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
S1G:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grntm1Blrl
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
S1H:
postnatal day 7; Grntm1Blrl/Grn +
(more )
Note: Double labeled: blue - Grn reporter; red - Grn protein (assay MGI:5620507).
Specimen
S1H:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Grn reporter; red - Grn protein (assay MGI:5620507).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
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S1H
,
S1J
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Co-labeling with anti-Grn antibody indicates that the knock-in reporter and the antibody label the identical set of cells.
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Specimen
Table 2:
postnatal month 3-4; Grntm1Blrl/Grn +
(more )
Specimen
Table 2:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Grntm1Blrl/Grn+
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Age:
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postnatal month 3-4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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