About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:217513 Espinoza-Lewis RA, et al., Generation of a Cre knock-in into the Myocardin locus to mark early cardiac and smooth muscle cell lineages. Genesis. 2014 Oct;52(10):879-87
Assay type: Recombinase reporter
MGI Accession ID: MGI:5615260
Gene symbol: Myocd
Gene name: myocardin
Reporter: lacZ
Direct Detection
Results
Specimen 3A E8.5: embryonic day 8.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS13: heart Present 3A E8.5

Specimen 3A E9.0: embryonic day 9.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS14: heart Present 3A E9.0

Specimen 3A E9.5: embryonic day 9.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: future midbrain Present 3A E9.5
TS15: heart Present 3A E9.5
TS15: somite Present 3A E9.5

Specimen 3A E10.5: embryonic day 10.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: heart Present 3A E10.5
TS17: midbrain Present 3A E10.5
TS17: somite Present 3A E10.5

Specimen 3A E13.5: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: heart Present 3A E13.5
TS21: midbrain Present 3A E13.5
TS21: somite Present 3A E13.5
TS21: limb Present 3A E13.5 proximal limb internal structures

Specimen 3A 15.5 left: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: embryo Present Regionally restricted 3A E15.5 left in the dorsal region

Specimen 3A 15.5 right: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: embryo Present Regionally restricted 3A E15.5 right dorsal region

Specimen 3B E10.5: embryonic day 10.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal section
Structure Level Pattern Image Note
TS17: heart atrium Present 3B stronger in the ventricle compared with the atrium
TS17: primitive ventricle Present 3B stronger in the ventricle compared with the atrium
TS17: somite Weak 3B compared to staining in the heart

Specimen 3C: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Sagital section
Structure Level Pattern Image Note
TS21: heart Present 3C
TS21: somite Present 3C in somite derivatives
TS21: blood vessel Present 3C in forming vasculature

Specimen 3D: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: heart Present 3D
TS23: lung Present 3D

Specimen 3E: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: lung Present 3E in the smooth muscle layer surrounding the forming bronchioles
TS23: smooth muscle tissue Present 3E in the smooth muscle layer surrounding the forming bronchioles

Specimen S2A: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Sectioned through the midline
Structure Level Pattern Image Note
TS23: embryo Present S2A
TS23: heart Present S2A

Specimen S2B: postnatal day 2; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal section
Structure Level Pattern Image Note
TS27: carotid artery Present S2B in the neointima layer

Specimen S2C: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal section
Structure Level Pattern Image Note
TS23: intestine muscularis Present S2C in the muscularis mucosae and externa of the small intestine

Specimen 5A E7.75: embryonic day 7.75; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Double labeled: blue - recombinase reporter; red - Isl1
Structure Level Pattern Image Note
TS11: heart Present 5A E7.75 KI lacZ staining is ventral to Isl1 staining

Specimen 5A E8.0 right: embryonic day 8.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Double labeled: blue - recombinase reporter; red - Isl1
Structure Level Pattern Image Note
TS12: primitive heart tube Present 5A E8.0 KI lacZ staining is ventral to Isl1 staining and at the poles

Specimen 5B R7.75 right: embryonic day 7.75; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal sections. Double labeled: blue - recombinase reporter; red - Isl1
Structure Level Pattern Image Note
TS11: heart Present 5B E7.75 KI arrowhead points to cells that stain for lacZ and Isl1

Specimen 5B E8.0 right: embryonic day 8.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal sections. Double labeled: blue - recombinase reporter; red - Isl1
Structure Level Pattern Image Note
TS12: primitive heart tube Present 5B E8.0 KI arrowhead points to cells that stain for lacZ and Isl1

Specimen 5C left: embryonic day 7.75; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal sections. Double labeled: blue - recombinase reporter; red - Isl1
Structure Level Pattern Image Note
TS11: heart Present 5C left horns of the forming cardiac tube with cells that stain for recombinase reporter and Isl1

Specimen 5C right: embryonic day 7.75; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal sections. Double labeled: blue - recombinase reporter; red - Isl1
Structure Level Pattern Image Note
TS11: heart Present 5C right medial arc of the forming cardiac tube with cells that stain for recombinase reporter and Isl1


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
12/30/2025
MGI 6.24
The Jackson Laboratory