Probe:	Fgf8 cDNA13
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Strong	Regionally restricted	6M	High expression level was detected in the commissural plate (the extreme anterior of the closing neural tube).
TS15: midbrain-hindbrain junction	Strong		6M
TS15: olfactory placode	Strong		6M
TS15: oral region ectoderm	Strong		6M

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Strong	Regionally restricted	6N	Elevated expression was detected in the commissural plate in the mutant.
TS15: midbrain-hindbrain junction	Strong		6N	Elevated expression was detected in the mutant.
TS15: olfactory placode	Strong		6N	Elevated expression was detected in the mutant.
TS15: oral region ectoderm	Strong		6N	Elevated expression was detected in the mutant.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Strong	Regionally restricted	6O	High expression level was detected in the commissural plate.
TS17: midbrain-hindbrain junction	Strong		6O
TS17: nasal pit	Strong		6O
TS17: oral region	Strong	Regionally restricted	6O	High expression level was detected in the oral ectoderm.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Strong	Regionally restricted	6P	Elevated expression was detected in the commissural plate in the mutant.
TS17: midbrain-hindbrain junction	Strong		6P	Elevated expression was detected in the mutant.
TS17: nasal pit	Strong		6P	Elevated expression was detected in the mutant.
TS17: oral region	Strong	Regionally restricted	6P	Elevated expression was detected in the oral ectoderm in the mutant.
TS17: diencephalon	Present	Regionally restricted	6P	Ectopic expression in the dorsal diencephalon was noted in some mutant embryos.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Strong	Regionally restricted	6Q	High expression level was detected in the commissural plate.
TS17: oral region	Strong	Regionally restricted	6Q	High expression level was detected in the oral ectoderm.
TS17: nasal pit	Strong		6Q

Structure	Level	Pattern	Image	Note
TS17: forebrain	Strong	Regionally restricted	6R	Elevated expression was detected in the commissural plate in the mutant.
TS17: oral region	Strong	Regionally restricted	6R	Elevated expression was detected in the oral ectoderm in the mutant.
TS17: nasal pit	Strong	Regionally restricted	6R	Elevated expression was detected in the mutant. The olfactory pits are more closely apposed and the rostral expression was continuous in the mutant.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Strong	Regionally restricted		High expression level was detected in the commissural plate.
TS19: oral region	Strong	Regionally restricted		High expression level was detected in the oral ectoderm.
TS19: midbrain-hindbrain junction	Strong
TS19: nasal pit	Strong

Structure	Level	Pattern	Image	Note
TS19: forebrain	Strong	Regionally restricted		Elevated expression was detected in the commissural plate in the mutant.
TS19: midbrain-hindbrain junction	Strong			Elevated expression was detected in the mutant.
TS19: nasal pit	Strong			Elevated expression was detected in the mutant.
TS19: oral region	Strong	Regionally restricted		Elevated expression was detected in the oral ectoderm in the mutant.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		8S
TS17: nasal pit	Present		8S
TS17: oral region	Present		8S

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		8T	Expression was increased in the mutant.
TS17: nasal pit	Present		8T	Expression was increased in the mutant.
TS17: oral region	Present		8T	Expression was increased in the mutant.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		8U	The Aln,Shh mutant embryos did not show the increased expression that was seen in Aln mutant embryos.
TS17: nasal pit	Present		8U	The Aln,Shh mutant embryos did not show the increased expression that was seen in Aln mutant embryos.
TS17: oral region	Present		8U	The Aln,Shh mutant embryos did not show the increased expression that was seen in Aln mutant embryos.