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Gene Expression Data
Assay Details
Assay
Reference: J:199568 Neufeld SJ, et al., mRNA fluorescence in situ hybridization to determine overlapping gene expression in whole-mount mouse embryos. Dev Dyn. 2013 Sep;242(9):1094-100
Assay type: RNA in situ
MGI Accession ID: MGI:5588436
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh cDNA3
Probe preparation: Antisense labelled with dinitrophenyl RNA
Visualized with: Cy3
Assay notes: Tyramide Signal Amplification was used.
Results
Specimen 2A: embryonic day 10.5 (more )
Note: Double labeled: red - Shh; green - Hoxd13 (assay MGI:5588380).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 2A , 2C Expression is in the posterior region.
Specimen 2D: embryonic day 10.5 (more )
Note: Double labeled: red - Shh; green - Hoxd13 (assay MGI:5588380).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 2F , 2D Expression is in the posterior region.
Specimen 2G: embryonic day 10.5 (more )
Note: Confocal image. Double labeled: red - Shh; green - Hoxd13 (assay MGI:5588380).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 2I , 2G Expression is in the posterior region.
Specimen 2J: embryonic day 10.5 (more )
Note: Double labeled: red - Shh; green - Hoxd13 (assay MGI:5588380).
Structure Level Pattern Image Note
TS17: hindgut Present Regionally restricted 2L , 2J Expression is in the posterior hindgut endoderm.
TS17: neural tube floor plate Present 2J , 2L
Specimen 2M: embryonic day 10.5 (more )
Note: Confocal image. Double labeled: red - Shh; green - Hoxd13 (assay MGI:5588380).
Structure Level Pattern Image Note
TS17: hindgut Present Regionally restricted 2M , 2O Expression is in the posterior hindgut endoderm.
TS17: neural tube floor plate Present 2O , 2M
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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