Pax6 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:203065 Sugiyama T, et al., The germinal matrices in the developing dentate gyrus are composed of neuronal progenitors at distinct differentiation stages. Dev Dyn. 2013 Dec;242(12):1442-53
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5587811
Gene symbol:	Pax6
Gene name:	paired box 6

Antibody:	anti-Pax6
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

2A ©

2B ©

2C ©

Specimen 2A: embryonic day 12.5 (more

)
Note: B-D: Higher magnification view of a boxed area in A. Double labeled: green - Pax6; red - Ephb1 (assay MGI:5587812).

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2C , 2B , 2A	Expression is stronger in the dentate neuroepithelium than in the cortical hem in the medial telencephalon.

2E ©

Specimen 2E: embryonic day 12.5 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2E	Expression is stronger in the dentate neuroepithelium than in the cortical hem in the medial telencephalon.

2F ©

Specimen 2F: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2F	Expression is stronger in the dentate neuroepithelium than in the cortical hem in the medial telencephalon.

3A ©

3B ©

Specimen 3A: embryonic day 12.5 (more

)
Note: Double labeled: green - Pax6; red - Pcna (assay MGI:5587813).

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3B , 3A	Expression is in the germinal matrices in the developing dentate gyrus.

3D ©

3E ©

Specimen 3D: embryonic day 14.5 (more

)
Note: Double labeled: green - Pax6; red - Pcna (assay MGI:5587813).

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		3E , 3D	Expression is in the germinal matrices in the developing dentate gyrus.
TS22: hippocampus	Present		3E , 3D

3G ©

3H ©

3J ©

Specimen 3G: embryonic day 16.5 (more

)
Note: Double labeled: green - Pax6; red - Pcna (assay MGI:5587813).

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted	3J , 3H , 3G	Expression is in the germinal matrices in the developing dentate gyrus (strong in the primary germinal matrix).

3L ©

3M ©

3O ©

Specimen 3L: embryonic day 18.5 (more

)
Note: Double labeled: green - Pax6; red - Pcna (assay MGI:5587813).

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present	Regionally restricted	3O , 3L , 3M	Expression is in the germinal matrices (strong in the primary matrix) and in the dental migratory stream.

3Q ©

Specimen 3Q: postnatal day 5 (more

)
Note: Double labeled: green - Pax6; red - Pcna (assay MGI:5587813).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	3Q	Expression is evenly in the tertiary germinal matrices.

3R ©

Specimen 3R: postnatal day 10 (more

)
Note: Double labeled: green - Pax6; red - Pcna (assay MGI:5587813).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	3R	Expression is in the tertiary germinal matrix.
TS28: dentate gyrus subgranular zone	Present		3R

3S ©

Specimen 3S: postnatal day 20 (more

)
Note: Double labeled: green - Pax6; red - Pcna (assay MGI:5587813).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		3S

4G ©

Specimen 4G: embryonic day 14.5 (more

)
Note: Double labeled: green - Pax6; red - Prox1 (assay MGI:5587816).

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	4G	Expression is in the primary germinal matrix of the forming dentate gyrus.

4H ©

Specimen 4H: embryonic day 16.5 (more

)
Note: Double labeled: green - Pax6; red - Prox1 (assay MGI:5587816).

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted	4H	Expression is in the tertiary germinal matrix of the forming dentate gyrus.

4I ©

Specimen 4I: embryonic day 18.5 (more

)
Note: Double labeled: green - Pax6; red - Prox1 (assay MGI:5587816).

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present	Regionally restricted	4I	Expression is in the tertiary germinal matrix.

4J ©

Specimen 4J: postnatal day 5 (more

)
Note: Double labeled: green - Pax6; red - Prox1 (assay MGI:5587816).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	4J	Expression is in the tertiary germinal matrix.

4K ©

Specimen 4K: postnatal day 10 (more

)
Note: Double labeled: green - Pax6; red - Prox1 (assay MGI:5587816).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		4K
TS28: dentate gyrus	Present	Regionally restricted	4K	Expression is in the tertiary germinal matrix.

4L ©

Specimen 4L: postnatal day 20 (more

)
Note: Double labeled: green - Pax6; red - Prox1 (assay MGI:5587816).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		4L

5A ©

Specimen 5A: embryonic day 14.5 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	5A	Expression is in the primary and secondary germinal matrices in the developing dentate gyrus.

5C ©

Specimen 5C: embryonic day 14.5 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	5C	Expression is in the primary and secondary germinal matrices in the developing dentate gyrus.

5E ©

Specimen 5E: embryonic day 16.5 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted	5E	Expression is in the primary and secondary germinal matrices in the developing dentate gyrus.

5G ©

Specimen 5G: embryonic day 16.5 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted	5G	Expression is in the primary and secondary germinal matrices in the developing dentate gyrus.

5I ©

Specimen 5I: embryonic day 16.5 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted	5I	Expression is in the tertiary germinal matrix of the forming dentate gyrus.

5K ©

Specimen 5K: embryonic day 16.5 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted	5K	Expression is in the tertiary germinal matrix of the forming dentate gyrus.

5M ©

Specimen 5M: embryonic day 18.5 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present		5M	Expression is in the secondary germinal matrix.

5O ©

Specimen 5O: embryonic day 18.5 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present		5O	Expression is in the secondary germinal matrix.

5Q ©

5U ©

Specimen 5Q: embryonic day 18.5 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present	Regionally restricted	5U , 5Q	Expression is in the tertiary germinal matrix.

5S ©

5W ©

Specimen 5S: embryonic day 18.5 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present	Regionally restricted	5S , 5W	Expression is in the tertiary germinal matrix.

6A ©

6a left ©

6a middle ©

6b left ©

6b middle ©

Specimen 6A: postnatal day 5 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	6b left , 6a left , 6A , 6b middle , 6a middle	Expression is predominantly surrounding the granule cell layer.
TS28: dentate gyrus granule cell layer	Weak		6b middle , 6A , 6a middle , 6b left , 6a left

6B ©

6c left ©

6c middle ©

6d left ©

6d middle ©

Specimen 6B: postnatal day 5 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	6B , 6c left , 6d middle , 6d left , 6c middle	Expression is predominantly surrounding the granule cell layer.
TS28: dentate gyrus granule cell layer	Weak		6d left , 6c middle , 6B , 6c left , 6d middle

6D ©

6g left ©

6g middle ©

Specimen 6D: postnatal day 10 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	6g left , 6g middle , 6D	Expression is predominantly surrounding the granule cell layer.
TS28: dentate gyrus subgranular zone	Present		6g left , 6g middle , 6D

6E ©

6h left ©

6h middle ©

Specimen 6E: postnatal day 10 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	6h middle , 6E , 6h left	Expression is predominantly surrounding the granule cell layer.
TS28: dentate gyrus subgranular zone	Present		6h middle , 6h left , 6E

6G ©

6j left ©

6j middle ©

Specimen 6G: postnatal day 20 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		6j middle , 6j left , 6G

6H ©

6k left ©

6k middle ©

Specimen 6H: postnatal day 20 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		6H , 6k middle , 6k left

7A ©

Specimen 7A: postnatal day 5 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	7A	Expression is in the lateral end of the lower blade of the dentate gyrus.

7B ©

Specimen 7B: postnatal day 5 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	7B	Expression is in the lateral end of the lower blade of the dentate gyrus.

7D ©

Specimen 7D: postnatal day 10 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	7D	Expression is in the lateral end of the lower blade of the dentate gyrus.

7E ©

Specimen 7E: postnatal day 10 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	7E	Expression is in the lateral end of the lower blade of the dentate gyrus.

7G ©

Specimen 7G: postnatal day 20 (more

)
Note: Double labeled: green - Pax6; red - Sox2 (assay MGI:5587815).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		7G

7H ©

Specimen 7H: postnatal day 20 (more

)
Note: Double labeled: green - Pax6; red - Neurod1 (assay MGI:5587817).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		7H

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