Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:84056 Motoyama J, et al., Differential requirement for Gli2 and Gli3 in ventral neural cell fate specification. Dev Biol. 2003 Jul 1;259(1):150-61
Assay type:	RNA in situ
MGI Accession ID:	MGI:5575362
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

2A ©

Specimen 2A: embryonic day 9.5 (more

)
Note: This section is at the thoracic level.

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		2A
TS15: notochord	Present		2A

2C ©

Specimen 2C: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: This section is at the thoracic level.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2C	The expression domain is expanded in this mutant.
TS15: neural tube floor plate	Present	Regionally restricted	2C	The expression domain is expanded in this mutant.
TS15: notochord	Present	Regionally restricted	2C	The expression domain is expanded in this mutant.

2E ©

Specimen 2E: embryonic day 9.5 (more

)
Note: This section is at the level of the trunk.

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present	Regionally restricted	2E
TS15: notochord	Present	Regionally restricted	2E

2G ©

Specimen 2G: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: This section is at the level of the trunk.

Structure	Level	Image	Note
TS15: neural tube	Present	2G	The expression domain is expanded in this mutant.
TS15: neural tube floor plate	Present	2G	The expression domain is expanded in this mutant.
TS15: notochord	Present	2G	Expression in this tissue is the same as in wild-type embryos.

2I ©

Specimen 2I: embryonic day 9.5 (more

)
Note: This section is at the tail level.

Structure	Level	Pattern	Image	Note
TS15: notochord	Present		2I

2K ©

Specimen 2K: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: This section is at the tail level.

Structure	Level	Pattern	Image	Note
TS15: notochord	Present		2K	Expression in this tissue is the same as in wild-type embryos.

4A ©

Specimen 4A: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		4A
TS15: notochord	Present		4A

4A1 ©

Specimen 4A1: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		4A1
TS15: notochord	Present		4A1

4A2 ©

Specimen 4A2: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		4A2
TS15: notochord	Present		4A2

4C ©

Specimen 4C: embryonic day 9.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		4C
TS15: future midbrain	Present		4C

4C1 ©

Specimen 4C1: embryonic day 9.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		4C1

4C2 ©

Specimen 4C2: embryonic day 9.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		4C2

4E ©

Specimen 4E: embryonic day 9.5; Gli2^tm1Alj/Gli2^tm1Alj, Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present	Regionally restricted	4E	Expression is present at the thoracic level and is absent at the level of the turnk and tail.
TS15: future forebrain	Present		4E	The expression domain is expanded in this mutant.

4E1 ©

Specimen 4E1: embryonic day 9.5; Gli2^tm1Alj/Gli2^tm1Alj, Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4E1	Expression is at the thoracic neural tube at the midline.
TS15: neural tube floor plate	Present	Regionally restricted	4E1	Expression is at the thoracic neural tube at the midline.

4E2 ©

Specimen 4E2: embryonic day 9.5; Gli2^tm1Alj/Gli2^tm1Alj, Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4E2	Expression is absent in the caudal neural tube (shown), present at the throacic level.

4G ©

Specimen 4G: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong		4G

4G1 ©

Specimen 4G1: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong		4G1

4G2 ©

Specimen 4G2: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong		4G2

5F ©

Specimen 5F: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	5F	Expression is at the midline.
TS17: forebrain	Present	Regionally restricted	5F	Expression is in the zona limitans intrathalamica.

5G ©

Specimen 5G: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	5G	Expression is ventral.
TS17: forebrain	Present	Regionally restricted	5G	Expression is in the zona limitans intrathalamica.

5H ©

Specimen 5H: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J, Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Weak	Regionally restricted	5H	Expression is ventral.
TS17: forebrain	Weak	Regionally restricted	5H	Expression is in the zona limitans intrathalamica.

5I ©

Specimen 5I: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3⁺, Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	5I	Expression is ventral.
TS17: forebrain	Present	Regionally restricted	5I	Expression is in the zona limitans intrathalamica and is expanded anterior to it.

5J ©

Specimen 5J: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	5J	The expression domain is expanded in this mutant.

5K ©

Specimen 5K: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	5K	Expression is at the midline.
TS17: forebrain	Present	Regionally restricted	5K	Expression is in the zona limitans intrathalamica.

5L ©

Specimen 5L: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	5L	Expression is ventral.
TS17: forebrain	Present	Regionally restricted	5L	Expression is in the zona limitans intrathalamica.

5M ©

Specimen 5M: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J, Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Weak	Regionally restricted	5M	Expression is ventral.
TS17: forebrain	Weak	Regionally restricted	5M	Expression is in the zona limitans intrathalamica.

5N ©

Specimen 5N: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3⁺, Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	5N	Expression is ventral.
TS17: forebrain	Present	Regionally restricted	5N	Expression is in the zona limitans intrathalamica and is expanded in the midline anterior to it.

5O ©

Specimen 5O: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Ptch1^tm1Mps/Ptch1^tm1Mps (more

)

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	5O	The expression domain is expanded in this mutant.

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