Probe:	Lpar1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present		1A
TS13: somite	Present		1A
TS13: trunk unsegmented mesenchyme	Present		1A

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Very strong	Regionally restricted	1B	Expression is in the rostral forebrain.
TS13: future brain neural fold	Weak		1B

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Present	Regionally restricted	1C	Expression is at the level of the prospective midbrain and rostral hindbrain in the dorsolateral region abutting the margins of the neural folds in the presumptive location of migratory neural crest cells.
TS13: migrating neural crest	Present	Regionally restricted	1C	Expression is at the level of the prospective midbrain and rostral hindbrain in the dorsolateral region abutting the margins of the neural folds in the presumptive location of migratory neural crest cells.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	2A	Diffuse expression in the lateral head mesenchyme.
TS15: somite	Present		2A
TS15: tail unsegmented mesenchyme	Present		2A

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Very strong		3A
TS17: 1st branchial arch maxillary component	Very strong		3A
TS17: 2nd branchial arch	Very strong		3A
TS17: limb	Very strong		3A
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	3A , 3A inset a	Expression is absent in the most anterior part.
TS17: somite	Present		3A inset a , 3A
TS17: neural tube floor plate	Present		3A inset b , 3A

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	4A	Expression is in the craniofacial region.
TS20: limb	Present		4A

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	5A	Expression is in the medial canthus.
TS20: pinna	Present		5A
TS20: forelimb mesenchyme	Very strong	Regionally restricted	5C , 5A	Expression is in the proximal region.
TS20: forelimb digit	Very strong		5C , 5A
TS20: forelimb interdigital region	Absent		5A , 5C

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	5B
TS20: limb	Present	Regionally restricted	5B

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	5E	Expression is in the medial canthus.
TS20: pinna	Present		5E

Structure	Level	Pattern	Image	Note
TS20: head	Present		5F

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	5G	Expression is in cervical mesenchyme.
TS20: mandible primordium	Present		5G
TS20: nose	Present		5G
TS20: tongue	Present		5G

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present		5H
TS20: somite	Present	Regionally restricted	5H	Expression is in posterior somites.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	10A	Expression is in the roof of the neopallial cortex.
TS20: head mesenchyme	Present	Regionally restricted	10A	Expression is in cervical mesenchyme an dthe perioccular region.
TS20: ear	Present		10A
TS20: mandible primordium	Present		10A

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	10A'	Expression is in the posterior tuberculum and in the wall.
TS20: foregut	Present	Regionally restricted	10A'	Expression is in the mesenchyme in the vicinity of the esophagus and thyroid duct.
TS20: ear	Present		10A'
TS20: mandible primordium	Present		10A'
TS20: tongue	Present		10A'
TS20: head mesenchyme	Present	Regionally restricted	10A'	Expression is in cervical mesenchyme an dthe perioccular region.

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Present		S2A
TS19: limb	Present		S2A
TS19: maxillary process	Present		S2A
TS19: nasal process	Present		S2A
TS19: somite	Present		S2A
TS19: telencephalon	Present		S2A
TS19: head mesenchyme	Present		S2A	Including the perioccular region.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	S3A	Expression is at the medial canthus and restricted expression in mesenchyme around the eye.
TS20: eye	Present	Regionally restricted	S3A	Expression is in the upper eyelid.

Structure	Level	Pattern	Image	Note
TS20: limb digit	Present		S3E
TS20: limb interdigital region	Absent		S3E

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted		Expression is in the head/face mesenchyme, periocular region, and periotic region.
TS20: genital tubercle	Present
TS20: limb	Present
TS20: mandible primordium	Present
TS20: nose	Present
TS20: tongue	Present
TS20: somite	Present	Regionally restricted		Expression is in posterior somites.