Antibody:	anti-HDAC2
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Graded	2C , 2A	Expressed throughout the brain. Robust expression in the outer layers of the developing neocortex, olfactory bulb and midbrain, with a lower expression in proliferating cells immediately adjacent to the lateral ventricle.
TS21: midbrain	Present	Graded	2C , 2A	Most robustly detected in cells in the outer layers, with a lower expression in proliferating cells immediately adjacent to the lateral ventricle.
TS21: neocortex	Present	Graded	2C , 2A	Most robustly detected in cells in the outer layers, with a lower expression in proliferating cells immediately adjacent to the lateral ventricle.
TS21: olfactory lobe	Present	Graded	2C , 2A	Most robustly detected in cells in the outer layers, with a lower expression in proliferating cells immediately adjacent to the lateral ventricle.
TS21: telencephalon	Present	Graded	2C , 2A	Most robustly detected in cells in the outer layers, with a lower expression in proliferating cells immediately adjacent to the lateral ventricle.
TS21: nasal capsule	Absent		2A , 2C	No expression in the cartilage around the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Widespread	2D
TS21: cerebral cortex marginal layer	Present		2D
TS21: cerebral cortex subventricular zone	Present		2D
TS21: cerebral cortex ventricular layer	Present		2D
TS21: cortical plate	Present		2D

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex marginal layer	Present		2E
TS21: cerebral cortex subventricular zone	Present		2E
TS21: cerebral cortex ventricular layer	Present		2E

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex marginal layer	Present		2F
TS21: cerebral cortex subventricular zone	Present		2F
TS21: cerebral cortex ventricular layer	Present		2F
TS21: cortical plate	Present		2F

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	2G	Weakly expressed in PCNA-positive progenitors around the rostral tip of the lateral ventricle, but is highly expressed in the developing olfactory bulb.
TS21: olfactory lobe	Strong		2G

Structure	Level	Pattern	Image	Note
TS21: olfactory lobe	Strong		2H	Highly expressed in Dcx-positive neuroblasts of the developing olfactory bulb.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	2I , 2J	Expressed around the 4th Ventricle, extending into the Dcx-positive neuroblasts of the hindbrain. But not detected within most Dcx-negative migrating cells.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present		2K	Expressed in NeuN-positive neurons.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Strong	Regionally restricted	2L , 2M	Highly expressed in neurons located ventrally to the central canal. Occasionally detected in PCNA-positive cells adjacent to the central canal.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Strong	Regionally restricted	2N	Highly expressed in NeuN-positive neurons located ventrally to the central canal.

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Strong	Widespread	4A	Highly expressed throughout the neuronal layers.
TS28: hippocampus	Strong	Widespread	4A
TS28: corpus callosum	Absent		4A

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Widespread	4B
TS28: main olfactory bulb	Strong	Widespread	4B
TS28: telencephalon	Strong	Regionally restricted	4B	Highly expressed throughout the neuronal layers of the cortex, hippocampus, olfactory bulb, and cerebellum, but it is rarely detected in the corpus callosum, lateral ventricle, or the rostral migratory stream.
TS28: rostral migratory stream	Trace		4B

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong	Widespread	4C

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Widespread	4D	Expressed in NeuN-positive neurons throughout the hippocampus.
TS28: dentate gyrus	Present		4D	Expressed in NeuN-negative immature granule neurons.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		4E	Rarely detected in PCNA-positive cells.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	4F	Expressed in NeuN-positive neurons, and occasional NeuN-negative nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		4G	Expressed in NeuN-positive neurons.
TS28: cerebellum Purkinje cell layer	Present		4G	Expressed in NeuN-negative neurons.
TS28: cerebellum external granule cell layer	Present		4G	Expressed in NeuN-negative neurons.
TS28: cerebellum molecular layer	Present		4G	Expressed in NeuN-negative neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present	Widespread	4I , 4H	Expressed in PCNA-positive cells in the outer part as well as in PCNA-negative cells in the inner part.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Widespread	4J , 4K	Expressed in NeuN-positive neurons throughout the olfactory bulb, as well as NeuN-negative mitral cells and periglomerular neurons.
TS28: main olfactory bulb glomerular layer	Present		4K , 4J
TS28: main olfactory bulb granule cell layer	Present		4K , 4J
TS28: main olfactory bulb mitral cell layer	Present		4K , 4J

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	4L	Not expressed in GFAP-positive glia around the dentate gyrus.
TS28: dentate gyrus	Present		4L

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	4M , 4N	Not expressed in GFAP-positive glia along the hippocampal formation and corpus callosum.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong	Regionally restricted	5D	Expressed in the the neuronal layers.
TS28: cerebral cortex	Strong	Widespread	5D
TS28: corpus callosum	Absent		5D
TS28: hippocampal formation	Present	Regionally restricted	5D	Not expressed in the white matter.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		5E	Expressed in the majority of NeuN-positive neurons.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Regionally restricted	5F	Not expressed in GFAP-positive astrocytes in the white matter.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Widespread	5J	Expressed throughout the neuronal layers.
TS28: cerebellum granule cell layer	Present	Widespread	5J , 5K	Expressed in NeuN-positive granule neurons.
TS28: cerebellum Purkinje cell layer	Present	Widespread	5K , 5J	Expressed in NeuN-negative neurons.
TS28: cerebellum molecular layer	Present	Widespread	5J	Expressed in neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		5L	Not expressed in GFAP-positive glia around the Purkinje cell layer.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	5N	Co-expressed with NeuN throughout the cortex.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Widespread	5R	Expressed throughout the neuronal layers.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Present		5S	Expressed in NeuN-positive granule neurons.
TS28: main olfactory bulb mitral cell layer	Present		5S	Expressed in large, NeuN-negative mitral cells.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		5T	Expressed in the periglomerular neurons.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		6O	Not expressed in most GFAP-positive progenitors.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		6P	Not expressed in most nestin-positive progenitors.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		6R , 6Q	Only weakly expressed in some PCNA-positive mitotic progenitors.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Strong		6S	Expressed in Dcx-positive neuroblasts.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	6T	Not expressed in the nestin-positive progenitors lining the subventricular zone of the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6U	Expressed in the rostral migratory stream, in which the expression was weak relative to the adjacent neurons.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6W , 6V	Expressed in PCNA-positive progenitors in the rostral migratory stream, in which the expression was weak relative to the adjacent neurons and neuroblasts.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6X	Expressed in the rostral migratory stream, in which the expression was weak relative to the adjacent Dcx-positive neuroblasts.