Antibody:	anti-HDAC1
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong	Graded	1A , 1C	Highly expressed in proliferative cells (PCNA positive) around the lateral ventricle, with relative detection decreasing in cells further away.
TS21: midbrain	Present	Scattered	1A , 1C	Only expressed in scattered cells. Not detected in most cells.
TS21: olfactory epithelium	Present		1A , 1C	Expressed in the proliferative olfactory epithelium (PCNA positive).

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong		1D	Highly expressed around the rostral tip.
TS21: olfactory lobe	Strong		1D

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong		1E	Highly expressed around the rostral tip.
TS21: olfactory lobe	Strong		1E

Structure	Level	Pattern	Image	Note
TS21: olfactory lobe	Present	Regionally restricted	1F , 1G	Expression is most robust in cells around the lateral ventricle. Not expressed in Dcx-positive neuroblasts that migrate away from the ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	1H , 1I	Expressed adjacent to the central canal.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	1J	Not expressed in late post-mitotic neurons (NeuN positive).

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Strong	Regionally restricted	1K	Highly expressed around the 4th Ventricle.

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Present		1L
TS21: pons	Present		1L
TS21: hindbrain	Present	Regionally restricted	1L , 1M	Expressed in chains of nuclei that appear to migrate out from 4th ventricle in channels flanked by Dcx-positive cells.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present		1N	Many cells co-express PCNA.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Scattered	3A
TS28: corpus callosum	Present	Scattered	3A
TS28: hippocampus	Present	Scattered	3A

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Scattered	3B
TS28: main olfactory bulb	Present	Scattered	3B
TS28: telencephalon	Present	Scattered	3B	Expressed in cells around the lateral ventricle.
TS28: rostral migratory stream	Present	Scattered	3B

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Scattered	3C	Expressed in cells in multiple layers.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		3D	Expressed in some NeuN-positive neurons.
TS28: hippocampus CA2	Present		3D	Expressed in some NeuN-positive neurons.
TS28: hippocampus CA3	Present		3D	Expressed in some NeuN-positive neurons.
TS28: hippocampus CA1	Absent		3D
TS28: hippocampus	Present		3D	Also expressed in non-neuronal cells around the dentate gyrus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		3E	Expressed in PCNA-positive cells.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3F	Expressed in small nuclei immediately adjacent to NeuN-positive neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		3G	Not highly detected within the neuronal nuclei.
TS28: cerebellum external granule cell layer	Strong		3G	Highly expressed in the proliferative progenitors.
TS28: cerebellum	Strong	Regionally restricted	3G	Expressed in the proliferative progenitors of the external granule layer, as well as NeuN-negative cells immediately adjacent to the Purkinje cell layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		3H	Expressed in NeuN-negative cells around the Purkinje cell layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		3I	Expressed in nestin-positive bergmann glia.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present	Scattered	3J
TS28: main olfactory bulb granule cell layer	Present	Scattered	3J
TS28: main olfactory bulb mitral cell layer	Present	Scattered	3J

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present	Scattered	3K	Do not co-express NeuN.
TS28: main olfactory bulb granule cell layer	Present	Scattered	3K	Do not co-express NeuN.
TS28: main olfactory bulb mitral cell layer	Present	Scattered	3K	Do not co-express NeuN.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	3L	Coexpressed with GFAP in the white matter surrounding the hippocampal formation.

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Present		3M , 3N	Coexpressed with GFAP.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Scattered	5A	Expressed in scattered cells throughout the cortex.
TS28: hippocampus	Present	Scattered	5A	Expressed in scattered cells throughout the hippocampus, but not the neuronal layers of CA3.
TS28: corpus callosum	Present	Scattered	5A	Expressed in scattered cells throughout the corpus callosum.
TS28: hippocampus CA1	Present		5A
TS28: hippocampus CA2	Present		5A
TS28: hippocampus CA3	Ambiguous		5A	Largely absent.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		5B	Expressed in some NeuN-positive neurons, and NeuN-negative cells in the subgranular zone.
TS28: dentate gyrus subgranular zone	Present		5B

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong		5C	Expressed in GFAP-positive glia in the white matter.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	5G	Expression borders the granule and Purkinje cell layers.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	5H	Expression borders the granule and Purkinje cell layers, where it is excluded from NeuN-positive neurons.
TS28: cerebellum granule cell layer	Present	Scattered	5H	Expressed in NeuN-negative cells.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	5I	Expression borders the granule and Purkinje cell layers, where it is co-expressed with GFAP.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5M	Expressed in non-neuronal cells, and in nuclei adjacent to NeuN-positive neurons.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Scattered	5O	Expressed in scattered, NeuN-negative non-neuronal cells throughout the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Present		5P	A subpopulation co-express the oligodendrocyte marker CNPase.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		5Q	A subpopulation co-express the oligodendrocyte marker CNPase.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		6A	Expressed in GFAP-positive cells.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		6B	Expressed in GFAP-positive cells.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		6C	Expressed in nestin-positive progenitors.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		6D	Expressed in rare actively cycling, PCNA-positive progenitors.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		6E	Not expressed in Dcx-positive neuroblasts.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Strong	Regionally restricted	6F	Expressed in the progenitor regions of the subventricular zone of the lateral ventricle and the rostral migratory stream, which coexpressed PCNA.
TS28: telencephalon subventricular zone	Strong	Regionally restricted	6F	Expressed in the progenitor regions of the subventricular zone of the lateral ventricle and the rostral migratory stream, which coexpressed PCNA.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6G	Expressed in PCNA-positive cycling progenitors in the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6H	Expressed along the rostral migratory stream, but not in most Dcx-positive neuroblasts.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6I	Expressed in GFAP-positive cells lining the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Strong	Regionally restricted	6J	Expressed along the subventricular zone of the lateral ventricle, in both GFAP-positive and GFAP-negative progenitors.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Strong	Regionally restricted	6K	Expressed along the subventricular zone of the lateral ventricle, in both GFAP-positive and GFAP-negative progenitors.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	6L	Expressed along the subventricular zone of the lateral ventricle, in nestin-positive progenitors.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	6M	Expressed along the subventricular zone of the lateral ventricle, in PCNA-positive mitotic progenitors.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	6N	Expressed along the subventricular zone of the lateral ventricle, but not in most Dcx-positive neuroblasts.