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Gene Expression Data
Assay Details
Assay
Reference: J:139639 Juszynski M, et al., Development of lymphatic vessels in mouse embryonic and early postnatal hearts. Dev Dyn. 2008 Sep 24;237(10):2973-2986
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5559047
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to alkaline phosphatase
Results
Specimen 9a: embryonic day 16.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS24: heart Present 9a

Specimen 9b: embryonic day 16.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS24: heart Present 9b

Specimen 9c: embryonic day 16.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS24: heart Present 9c

Specimen 9d: embryonic day 16.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS24: arterial system Present Regionally restricted 9d Expressed in the mural cells of the coronary artery.

Specimen 12a: embryonic day 17.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS25: heart Present 12a

Specimen 12b: embryonic day 17.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS25: heart Present 12b

Specimen 12c: embryonic day 17.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS25: heart Present 12c

Specimen 12d: embryonic day 17.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS25: heart Present 12d

Specimen 12e: embryonic day 17.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS25: heart Present 12e

Specimen 12f: embryonic day 17.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS25: heart Present 12f

Specimen 12g: embryonic day 17.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS25: heart Present 12g

Specimen 12h: embryonic day 17.5 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS25: aorta Present 12h
TS25: heart Present 12h
TS25: pulmonary trunk Present 12h
TS25: lymphatic system Present Single cells 12h Expressed in cells partially covering a lymphatic vessel located between the pulmonary trunk and the aorta.

Specimen 13a: postnatal day 4 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS28: heart Present Scattered 13a Expressed in scattered groups of cells around lymphatic vessels between the aorta and the pulmonary trunk. Some smooth muscle cells are at a distance from lymphatic endothelium.
TS28: lymphatic vessel Present Scattered 13a Expressed in scattered groups of cells around lymphatic vessels between the aorta and the pulmonary trunk. Some smooth muscle cells are at a distance from lymphatic endothelium.
TS28: aorta Present 13a
TS28: pulmonary trunk Present 13a

Specimen 13b: postnatal day 4 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS28: heart Present Scattered 13b Expressed in scattered groups of cells around lymphatic vessels between the aorta and the pulmonary trunk.
TS28: lymphatic vessel Present Scattered 13b Expressed in scattered groups of cells around lymphatic vessels between the aorta and the pulmonary trunk.
TS28: aorta Present 13b
TS28: pulmonary trunk Present 13b

Specimen 13c: postnatal day 4 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS28: coronary artery Present 13c

Specimen 13d: postnatal day 4 (more )
Note: Fixed in 2-methylobuthane cooled in an acetone-dry ice mixture. Double labeled: navy blue - Acta2; brown - Lyve1 (assay MGI:5559017).
Structure Level Pattern Image Note
TS28: coronary artery Present 13d


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory