Antibody:	anti-Mouse LYVE-1 MAb (Clone 223322)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: liver sinusoid	Present		1a
TS19: vena cava	Present	Regionally restricted	1a , 1b	Expressed in the endothelial cells building partially the lumen of the proximal part of the left cranial caval vein.
TS19: anterior cardinal vein	Present	Regionally restricted	1a	Expressed in the left anterior cardinal vein.
TS19: heart	Present	Regionally restricted	1a , 1b	Scattered expression in the dorsal surface.
TS19: outflow tract	Present	Single cells	1a	Expressed in a few cells on the arterial pole of the heart.

Structure	Level	Pattern	Image	Note
TS19: left atrium endocardial lining	Present	Regionally restricted	1d , 1c	Expressed in the endocardial endothelial cells near the opening of the pulmonary vein.
TS19: venous system	Present	Regionally restricted	1c , 1d	Expressed in the epithelial cells of the pulmonary vein.
TS19: lymphatic system	Present	Regionally restricted	1d , 1c	Expressed in the lumen of a small vascular tubule on the surface of the right atrium.

Structure	Level	Pattern	Image	Note
TS19: vena cava	Present	Regionally restricted	1e , 1f	Expressed in the lumen of the proximal right anterior cardinal vein. Faint expression in the surface of the right venous valve.

Structure	Level	Pattern	Image	Note
TS21: heart	Present	Scattered	2a	Only expressed in a few cells in the sternal surface.

Structure	Level	Pattern	Image	Note
TS21: heart	Present	Scattered	2b	Expression was more numerous close to the base.

Structure	Level	Pattern	Image	Note
TS21: heart	Present	Scattered	2c	Expressed predominantly on the diaphragmatic surface.

Structure	Level	Pattern	Image	Note
TS21: heart ventricle	Present	Scattered	3a	Expressed in evenly scattered cells and cellular clusters on the surface.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present	Scattered	3b	Expressed in a few cells deeper in the myocardial wall on the diaphragmatic surface of the atrioventricular sulcus.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present		3c	Expressed in cells derived from epicardium, sprouting toward myocardium in some areas.

Structure	Level	Pattern	Image	Note
TS21: heart	Present	Regionally restricted	3d	Expressed in short tubules on the diaphragmatic surface of the heart and on the surface of coronary sinus.
TS21: lymphatic system	Present	Regionally restricted	3d	Expressed in short tubules on the diaphragmatic surface of the heart and on the surface of coronary sinus.

Structure	Level	Pattern	Image	Note
TS21: heart	Present	Regionally restricted	3e	Expressed in the wall of the coronary sinus. Cells penetrated from the endocardial.

Structure	Level	Pattern	Image	Note
TS21: heart	Present	Regionally restricted	3f	Expressed in the wall of the coronary sinus. Cells penetrated from the epicardial surface.

Structure	Level	Pattern	Image	Note
TS22: heart	Present	Scattered	4a	Expressed in scattered cells and lymphatic tubules resembling vessels on the diaphragmatic surface of the left and the right atrium and the coronary sinus.
TS22: lymphatic system	Present	Regionally restricted	4a	Expressed in lymphatic tubules resembling vessels on the diaphragmatic surface of the heart.
TS22: left atrium	Present	Scattered	4a	Expressed in scattered cells and lymphatic tubules resembling vessels on the diaphragmatic surface.
TS22: right atrium	Present	Scattered	4a	Expressed in scattered cells and lymphatic tubules resembling vessels on the diaphragmatic surface.
TS22: heart left ventricle	Present	Regionally restricted	4a	Expressed in discontinuous tubules arranged in a row.

Structure	Level	Pattern	Image	Note
TS22: heart	Present	Regionally restricted	4b	Expressed in scattered cells and lymphatic tubules resembling vessels on the diaphragmatic surface. Some tubules are formed; other tubules seem to coalesce from scattered positive cells arranged in a row.
TS22: lymphatic system	Present	Regionally restricted	4b	Expressed in lymphatic tubules resembling vessels on the diaphragmatic surface of the heart.

Structure	Level	Pattern	Image	Note
TS22: heart	Present	Scattered	4c	Expressed in scattered cells on the sternal surface.
TS22: lymphatic system	Present	Regionally restricted	4c	The first clusters of cell aggregates in the form of a lattice are seen on the roots of the aorta.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Present	Clusters	5a	Expressed in cellular clusters penetrating between the aorta and the pulmonary trunk.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Present	Regionally restricted	5b	Expressed in cellular clusters penetrating between the aorta and the pulmonary trunk. Expressed in discontinuous secondary vesicle on the surface of the aorta.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Present	Clusters	5c	Expressed in cellular clusters penetrating between the aorta and the pulmonary trunk.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Present	Clusters	5d	Expressed in cellular clusters penetrating between the aorta and the pulmonary trunk.

Structure	Level	Pattern	Image	Note
TS23: heart	Present	Regionally restricted	6a	Expressed in scattered clusters all over the heart. Expressed in lymphatic tubules running on the diaphragmatic surface of the left atrium, within the atrioventricular sulcus, on the left ventricle, and within the interventricular sulcus.
TS23: lymphatic system	Present	Regionally restricted	6a	Expressed in scattered clusters all over the heart. Expressed in lymphatic tubules running on the diaphragmatic surface of the left atrium, within the atrioventricular sulcus, on the left ventricle, and within the interventricular sulcus.

Structure	Level	Pattern	Image	Note
TS23: heart	Present	Regionally restricted	6b	Expressed in scattered clusters and lymphatic tubules on the diaphragmatic surface of the heart.
TS23: lymphatic system	Present	Regionally restricted	6b	Expressed in scattered clusters and lymphatic tubules on the diaphragmatic surface of the heart.

Structure	Level	Pattern	Image	Note
TS23: heart	Present	Regionally restricted	6c	Expressed in scattered clusters and lymphatic tubules on the sternal surface of the heart. The tubules appeared on the left side of the conus along the left conal vein.
TS23: lymphatic system	Present	Regionally restricted	6c	Expressed in scattered clusters and lymphatic tubules on the sternal surface of the heart. The tubules appeared on the left side of the conus along the left conal vein.

Structure	Level	Pattern	Image	Note
TS23: lymphatic system	Present	Regionally restricted	7a	Expressed cranially in a small amount clusters and tubules along the great arteries.

Structure	Level	Pattern	Image	Note
TS23: lymphatic system	Present	Regionally restricted	7b	Expressed cranially in a small amount clusters and tubules along the great arteries.

Structure	Level	Pattern	Image	Note
TS23: heart	Present	Regionally restricted	7c	Expressed in the lymphatic plexus of the heart at the level of the semilunar valves.
TS23: lymphatic system	Present	Regionally restricted	7c	Expressed in the lymphatic plexus of the heart at the level of the semilunar valves.

Structure	Level	Pattern	Image	Note
TS23: heart	Present	Regionally restricted	7d	Expressed in the lymphatic plexus of the heart at the level of the semilunar valves.
TS23: lymphatic system	Present	Regionally restricted	7d	Expressed in the lymphatic plexus of the heart at the level of the semilunar valves.

Structure	Level	Pattern	Image	Note
TS24: heart	Present	Regionally restricted	8b , 8a	Expressed in discontinuous tubules on the diaphragmatic surface of the heart. Expressed in collecting vessels on the surface of the left atrium, the coronary sinus and the atrioventricular sulcus.
TS24: lymphatic system	Present	Regionally restricted	8b , 8a	Expressed in discontinuous tubules on the diaphragmatic surface of the heart. Expressed in collecting vessels on the surface of the left atrium, the coronary sinus and the atrioventricular sulcus.

Structure	Level	Pattern	Image	Note
TS24: heart	Present	Regionally restricted	8c	Expressed in lymphatic tubules, which assemble along the left conal vein on the sternal surface of the heart.
TS24: lymphatic system	Present	Regionally restricted	8c	Expressed in lymphatic tubules, which assemble along the left conal vein on the sternal surface of the heart.

Structure	Level	Pattern	Image	Note
TS24: heart	Present	Regionally restricted	8d	Expressed in discontinuous lattice of lymphatic capillary plexus on the surface of the pulmonary trunk and the aorta, spreading on the heart conus on the sternal surface.
TS24: lymphatic system	Present	Regionally restricted	8d	Expressed in discontinuous lattice of lymphatic capillary plexus on the surface of the pulmonary trunk and the aorta, spreading on the heart conus on the sternal surface.

Structure	Level	Pattern	Image	Note
TS24: left ventricle cardiac muscle	Present	Regionally restricted	9a	Expressed in scattered cells and lymphatic tubules within the myocardium.
TS24: right ventricle cardiac muscle	Present	Regionally restricted	9a	Expressed in scattered cells and lymphatic tubules within the myocardium.
TS24: heart left ventricle	Present	Regionally restricted	9a	Expressed in scattered cells and lymphatic tubules on the cardiac surface.
TS24: heart right ventricle	Present	Regionally restricted	9a	Expressed in scattered cells and lymphatic tubules on the cardiac surface.
TS24: heart	Present	Scattered	9a	Expressed in endothelial cells that did not seem to form a lumen on the surface of the coronary sinus and in the adjacent myocardium.
TS24: lymphatic system	Present	Regionally restricted	9a	Expressed in scattered cells and lymphatic tubules on the cardiac surface and within the myocardium.

Structure	Level	Pattern	Image	Note
TS24: heart	Present	Regionally restricted	9b	Expressed in cellular clusters forming lumen on the surface of the heart.
TS24: lymphatic system	Present	Regionally restricted	9b	Expressed in cellular clusters forming lumen on the surface of the heart.

Structure	Level	Pattern	Image	Note
TS24: heart	Present	Regionally restricted	9c	Expressed in scattered cells and lymphatic tubules in the coronary sinus area.
TS24: lymphatic system	Present	Regionally restricted	9c	Expressed in scattered cells and lymphatic tubules in the coronary sinus area.

Structure	Level	Pattern	Image	Note
TS24: heart	Present	Regionally restricted	9d	Expressed in cellular clusters forming lumen in the atrioventricular sulcus area.
TS24: lymphatic system	Present	Regionally restricted	9d	Expressed in cellular clusters forming lumen in the atrioventricular sulcus area.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	10a	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the cardiac caudal vein.
TS25: lymphatic system	Present	Regionally restricted	10a	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the cardiac caudal vein.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	10b	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the right cardiac vein.
TS25: lymphatic system	Present	Regionally restricted	10b	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the right cardiac vein.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	10c	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the left conal vein.
TS25: lymphatic system	Present	Regionally restricted	10c	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the left conal vein.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	10d	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the aorta.
TS25: lymphatic system	Present	Regionally restricted	10d	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the aorta.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	10e	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the left cardiac vein and the coronary sinus.
TS25: lymphatic system	Present	Regionally restricted	10e	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the left cardiac vein and the coronary sinus.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	10f	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the left conal vein.
TS25: lymphatic system	Present	Regionally restricted	10f	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart, forming a lattice of branching, continuous tubules around the left conal vein.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	10g	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart. The border of two collecting lymphatic systems: draining lymph from the left ventricle and the right ventricle lies near the heart apex at the interventricular sulcus.
TS25: lymphatic system	Present	Regionally restricted	10g	Expressed in tubules and lymphatic vessels on the subepicardial surface of the heart. The border of two collecting lymphatic systems: draining lymph from the left ventricle and the right ventricle lies near the heart apex at the interventricular sulcus.

Structure	Level	Pattern	Image	Note
TS24: heart	Present	Regionally restricted	11a	Expressed in scattered cells and lymphatic tubules on the surface of the heart. Scattered cells protruded toward the forming tubules.
TS24: lymphatic system	Present	Regionally restricted	11a	Expressed in scattered cells and lymphatic tubules on the surface of the heart. Scattered cells protruded toward the forming tubules.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	11b	Expressed in scattered cells and lymphatic tubules on the surface of the heart. Scattered cells protruded toward the existing tubules.
TS25: lymphatic system	Present	Regionally restricted	11b	Expressed in scattered cells and lymphatic tubules on the surface of the heart. Scattered cells protruded toward the existing tubules.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	11c	Expressed in scattered cells and lymphatic tubules on the surface of the heart. There were single scattered cells located within the openings of the lymphatic lattice.
TS25: lymphatic system	Present	Regionally restricted	11c	Expressed in scattered cells and lymphatic tubules on the surface of the heart. There were single scattered cells located within the openings of the lymphatic lattice.

Structure	Level	Pattern	Image	Note
TS25: ventricle endocardial lining	Present	Regionally restricted	12a	Expressed in endocardial endothelial cells within the myocardium in both ventricles.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	12b	Expressed in cells accumulated above the mitral valve.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	12c	Expressed in cells accumulated in the atrioventricular sulcus.

Structure	Level	Pattern	Image	Note
TS25: interventricular groove	Present	Clusters	12d	Expressed in clusters without yet forming a lumen in the interventricular sulcus around the major caudal vein.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	12e	Expressed in a lattice of lymphatic vessels at the roots of the aorta and the pulmonary trunk.
TS25: lymphatic system	Present	Regionally restricted	12e	Expressed in a lattice of lymphatic vessels at the roots of the aorta and the pulmonary trunk.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	12f	Expressed in an exuberant lattice of lymphatic vessels around the base of the coronary sinus.
TS25: lymphatic system	Present	Regionally restricted	12f	Expressed in an exuberant lattice of lymphatic vessels around the base of the coronary sinus.
TS25: endocardial tissue	Present	Regionally restricted	12f	Expressed in endocardial endothelial cells within the coronary sinus.

Structure	Level	Pattern	Image	Note
TS25: left atrium endocardial lining	Present	Regionally restricted	12g	Expressed in a population of endocardial endothelial cells.

Structure	Level	Pattern	Image	Note
TS25: heart	Present	Regionally restricted	12h	Expressed in lymphatic vessels located between the pulmonary trunk and the aorta.
TS25: lymphatic system	Present	Regionally restricted	12h	Expressed in lymphatic vessels located between the pulmonary trunk and the aorta.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	13a	Expressed in lymphatic vessels located between the pulmonary trunk and the aorta.
TS28: lymphatic vessel	Present	Regionally restricted	13a	Expressed in lymphatic vessels located between the pulmonary trunk and the aorta.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	13b	Expressed in lymphatic vessels located between the pulmonary trunk and the aorta.
TS28: lymphatic vessel	Present	Regionally restricted	13b	Expressed in lymphatic vessels located between the pulmonary trunk and the aorta.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	13c	Expressed in lymphatic vessels and tubules located around the major branches of coronary arteries.
TS28: lymphatic vessel	Present	Regionally restricted	13c	Expressed in lymphatic vessels and tubules located around the major branches of coronary arteries.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	13d	Expressed in lymphatic vessels and tubules located around the major branches of coronary arteries.
TS28: lymphatic vessel	Present	Regionally restricted	13d	Expressed in lymphatic vessels and tubules located around the major branches of coronary arteries.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Widespread	14a	Expressed in exuberant lattice of lymphatic vessels.
TS28: lymphatic vessel	Present	Widespread	14a	Expressed in exuberant lattice of lymphatic vessels.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	14b	Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance. No scattered cells.
TS28: lymphatic vessel	Present	Regionally restricted	14b	Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance. No scattered cells.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	14c	Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance. No scattered cells.
TS28: lymphatic vessel	Present	Regionally restricted	14c	Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance. No scattered cells.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	14d	Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance. No scattered cells.
TS28: lymphatic vessel	Present	Regionally restricted	14d	Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance. No scattered cells.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	14e	Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance. No scattered cells.
TS28: lymphatic vessel	Present	Regionally restricted	14e	Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance. No scattered cells.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	14f	Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance. No scattered cells.
TS28: lymphatic vessel	Present	Regionally restricted	14f	Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance. No scattered cells.

Structure	Level	Pattern	Image	Note
TS18: heart	Weak	Scattered		Faint expression in the trabecular myocardium and the subepicardium.

Structure	Level	Pattern	Image	Note
TS20: heart	Present	Scattered		Expression was more numerous as compared to those in 11 dpc, and was mostly located in the subepicardial area on the diaphragmatic surface.

Structure	Level	Pattern	Image	Note
TS23: lymphatic system	Present	Regionally restricted		Expressed in the tubules, which formed a plexus at the roots of the great arteries.

Structure	Level	Pattern	Image	Note
TS27: heart	Present	Regionally restricted		Expressed in lumenized vessels traversing along the interventricular sulcuses. Expressed in scattered cells within the subepicardium. Expressed in a net-like capillary plexus, extending further toward the apex and covered both ventricles and atria.
TS27: lymphatic vessel	Present	Regionally restricted		Expressed in lumenized vessels traversing along the interventricular sulcuses. Expressed in scattered cells within the subepicardium. Expressed in a net-like capillary plexus, extending further toward the apex and covered both ventricles and atria.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted		Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance.
TS28: lymphatic vessel	Present	Regionally restricted		Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted		Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance.
TS28: lymphatic vessel	Present	Regionally restricted		Expressed in increased number of lymphatic vessels, and apart from vessels of larger diameter there were thin-walled lymphatic vessels traversing the outer layer of the myocardial wall and having an irregularly beaded appearance.