Antibody:	Anti-Fat3
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	3A	Expression was detected in apical and basal dendrites.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	3B	Expression was detected in apical and basal dendrites.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	3C , 3C''	Expression overlapped with Map2.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	3D	Expression was detected in corticofugal axons, overlapping with Tag-1 (Cntn2) signal.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	3E	Expression was detected in the internal capsule.
TS22: thalamus	Present		3E

Structure	Level	Pattern	Image	Note
TS27: parietal cortex	Present	Regionally restricted	3F	Expression was detected in the parietal cortex area 1.
TS27: corpus callosum	Present		3F
TS27: optic tract	Present		3F
TS27: thalamus	Present		3F
TS27: midbrain	Present	Regionally restricted	3F	Expression was detected in the cerebral peduncle.
TS27: diencephalon	Present	Regionally restricted	3F	Expression was detected in the mammillothalamic tract.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted	3G	Expression was detected in the primitive plexiform layer.

Structure	Level	Pattern	Image	Note
TS27: hippocampus stratum lacunosum	Present		3H

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	3I	Expression was detected in the fasciculus retroflexus.

Structure	Level	Pattern	Image	Note
TS27: midbrain	Present	Regionally restricted	3J	Expression was detected in the fasciculus retroflexus, mammillotegmental tract, and mammillothalamic tract.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	3K	Expression was detected in the midbrain and the mammillotegmental tract.
TS22: spinal cord	Present	Regionally restricted	3K	Expression was detected in axon fibers.

Structure	Level	Pattern	Image	Note
TS27: midbrain	Present		3L

Structure	Level	Pattern	Image	Note
TS22: superior colliculus	Present		3M	Signal partially overlapped with L1-cam signal.

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Absent

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Absent

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	3N	Expression was detected in axon tracts or fascicles.

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Absent		3O

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4G	Expression was detected in the most rostral region of the cortex in the presumptive mitral cells.

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Present	Regionally restricted	4H	Expression was widely detected in the intermediate zone and in the mitral cell layer.

Structure	Level	Pattern	Image	Note
TS25: main olfactory bulb	Present	Regionally restricted	4I	Expression became concentrated in the external plexiform layer.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb external plexiform layer	Present		4K , 4J	Dot-like signals were noted on the dendrites of mitral cells.
TS27: main olfactory bulb glomerular layer	Present		4J

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	4L	Expression was detected in the lateral olfactory tract.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Trace

Structure	Level	Pattern	Image	Note
TS25: main olfactory bulb	Present	Regionally restricted	5A'' , 5A	Expression was detected in the dendrites and axons of mitral cells. There was no co-expression with Gap43 in the olfactory sensory neuron axons in the outer nuclear layer, but partial overlap was noted at the outer nuclear layer border.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb glomerular layer	Present		5B'' , 5B	Expression was detected over the glomeruli and overlapped with Gap43.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb external plexiform layer	Present	Regionally restricted	5C'' , 5C	Expression overlapped with Map2 on mitral cell dendrites.
TS27: main olfactory bulb glomerular layer	Present	Regionally restricted	5C'' , 5C	Expression overlapped with Map2 on mitral cell dendrites.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb internal plexiform layer	Present	Regionally restricted	5D , 5D''	Expression overlapped with Tau on mitral cell axons.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	6E	Expression was detected in the nerve fiber layer.

Structure	Level	Pattern	Image	Note
TS25: neural retina	Present	Regionally restricted	6F	Expression was detected in the inner plexiform layer.

Structure	Level	Pattern	Image	Note
TS28: retina inner plexiform layer	Present	Non-Uniform	6G , 6H	Expression in the inner plexiform layer reached a maximum at P11. Sublaminar structures were differentially labeled.

Structure	Level	Pattern	Image	Note
TS28: retina inner plexiform layer	Present		6I	Expression in the inner plexiform layer overlapped with Syntaxin.

Structure	Level	Pattern	Image	Note
TS28: retina	Absent

Structure	Level	Pattern	Image	Note
TS27: optic tract	Present		6J