Antibody:	anti-TH (AB1542)
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	Primary antibody diluted to 1:1000; secondary antibody diluted to 1:1000.

Structure	Level	Pattern	Image	Note
TS21: primitive bladder	Present	Graded	GUDMAP:21019	dorsal expression; Axons are present at very low density in dorsal outer aspect of bladder. Not seen in lamina propria or associated with the urothelium.
TS21: nerve of primitive bladder	Present	Graded	GUDMAP:21019	dorsal expression; Axons are present at very low density in dorsal outer aspect of bladder. Not seen in lamina propria or associated with the urothelium.

Structure	Level	Pattern	Image	Note
TS28: nerve of bladder	Present		GUDMAP:21194
TS28: nerve of lamina propria of bladder	Present		GUDMAP:21194	Primarily associated with vasculature
TS28: nerve of detrusor muscle of bladder	Present	Graded	GUDMAP:21194	Detrusor innervation more prominent in outer muscle.
TS28: nerve of outer layer of bladder	Present		GUDMAP:21194
TS28: nerve of urothelium of bladder	Absent		GUDMAP:21194

Structure	Level	Pattern	Image	Note
TS24: nerve of bladder	Present	Graded	GUDMAP:21213	dorsal expression; Strongly labeled axons on dorsal surface and in dorsal detrusor. Rare axons in ventral detrusor or ventral surface. None in lamina propria or associated with urothelium.
TS24: nerve of urothelium of bladder	Absent		GUDMAP:21213
TS24: nerve of lamina propria of bladder	Absent		GUDMAP:21213
TS24: nerve of detrusor muscle of bladder	Present	Graded	GUDMAP:21213	dorsal expression; Strongly labeled axons in dorsal detrusor. Rare axons in ventral detrusor.
TS24: nerve of outer layer of bladder	Present	Graded	GUDMAP:21213	dorsal expression; Strongly labeled axons on dorsal surface but rare on ventral surface.

Structure	Level	Pattern	Image	Note
TS28: nerve of prostate gland	Present		GUDMAP:21222
TS28: pelvic ganglion	Present		GUDMAP:21222
TS28: nerve of bladder	Present		GUDMAP:21222
TS28: nerve of detrusor muscle of bladder	Present		GUDMAP:21222
TS28: nerve of lamina propria of bladder	Present		GUDMAP:21222
TS28: nerve of outer layer of bladder	Present		GUDMAP:21222
TS28: nerve of urothelium of bladder	Absent		GUDMAP:21222

Structure	Level	Pattern	Image	Note
TS28: pelvic ganglion	Present		GUDMAP:21288
TS28: nerve of pelvic urethra of female	Present		GUDMAP:21288
TS28: nerve of bladder	Present		GUDMAP:21288
TS28: nerve of lamina propria of bladder	Present		GUDMAP:21288
TS28: nerve of detrusor muscle of bladder	Present		GUDMAP:21288
TS28: nerve of outer layer of bladder	Present		GUDMAP:21288
TS28: nerve of urothelium of bladder	Absent		GUDMAP:21288

Structure	Level	Pattern	Image	Note
TS26: nerve of bladder	Present		GUDMAP:21351
TS26: nerve of lamina propria of bladder	Present		GUDMAP:21351	Axons are labelled with moderate intensity but are quite sparsely distributed.
TS26: nerve of detrusor muscle of bladder	Present	Regionally restricted	GUDMAP:21351	dorsal expression; Axons are scarce in the detrusor, where they are primarily on the dorsal aspect.
TS26: nerve of outer layer of bladder	Present	Graded	GUDMAP:21351	dorsal expression; More prevalent on dorsal aspect of bladder
TS26: nerve of urothelium of bladder	Absent		GUDMAP:21351

Structure	Level	Pattern	Image	Note
TS26: nerve of lamina propria of bladder	Present	Uniform	GUDMAP:22913	TH positive nerves are seen more frequently towards the base of the bladder near where the ureters enter.
TS26: nerve of detrusor muscle of bladder	Present	Graded	GUDMAP:22913	Axon bundles and cell bodies are more prevalent on the dorsal side of the bladder.
TS26: nerve of outer layer of bladder	Absent		GUDMAP:22913
TS26: nerve of urethra	Present	Uniform	GUDMAP:22913
TS26: pelvic ganglion	Present		GUDMAP:22913
TS26: nerve of urothelium of bladder	Absent		GUDMAP:22913
TS26: nerve of ureter	Present	Uniform	GUDMAP:22913