Gene Expression Data
Assay Details
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Assay
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Reference:
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J:125514
Hartman BH, et al., Dll3 is expressed in developing hair cells in the mammalian cochlea. Dev Dyn. 2007 Oct;236(10):2875-83
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5547510
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Gene symbol:
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Dll3
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Gene name:
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delta like canonical Notch ligand 3
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Probe:
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MGC clone 62323
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Modified Carnoy's fixative: 60% ethanol/30% formaldehyde/10% acetic acid
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Results
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Specimen
1A,B,C:
embryonic day 15.5
(more  )
Specimen
1A,B,C:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea |
Present |
Non-Uniform |
1A
,
1B
,
1C
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Expression was detected in the presumptive inner hair cells, strongly in the basal turn (C) and at a low level in the mid-basal turn (B).
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| TS23: cochlea epithelium |
Present |
Regionally restricted |
1A
,
1B
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Expression was restricted to cells near the lumen.
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Specimen
1N,O:
embryonic day 15.5
(more )
Note: Triple labeled: blue - Dll3; red - Nefm (assay MGI:5547530); green - Cdkn1b (assay MGI:5547532).
Specimen
1N,O:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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Triple labeled: blue - Dll3; red - Nefm (assay MGI:5547530); green - Cdkn1b (assay MGI:5547532).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea |
Present |
Regionally restricted |
1N
,
1O
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Expression was detected in the presumptive inner hair cells.
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Specimen
2A,C:
embryonic day 14.5
(more )
Specimen
2A,C:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Specimen
3A,G:
embryonic day 17.0
(more )
Specimen
3A,G:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cochlea |
Present |
Regionally restricted |
3A
,
3G
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There was a base-to-apex gradient of expression in hair cells that extended to the middle turn. Expression level was low in inner hair cells and stronger in outer hair cells in the basal turn.
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Specimen
4A,C:
postnatal day 0
(more )
Specimen
4A,C:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
4C
,
4A
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Levels are strongest in the most apical hair cells, but expression is low or undetectable in hair cells in the more basal regions. Expression is stronger in outer hair cells than inner hair cells. Expression was present in the middle turn.
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Specimen
not shown P3:
postnatal day 3
(more )
Specimen
not shown P3:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
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Expression was detected in hair cells only in the most apical turn of the organ of Corti. Expression was absent from all hair cells in the middle and basal turns.
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Specimen
not shown P5:
postnatal day 5
(more )
Specimen
not shown P5:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Analysis Tools