Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:125514
Hartman BH, et al., Dll3 is expressed in developing hair cells in the mammalian cochlea. Dev Dyn. 2007 Oct;236(10):2875-83
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5547530
|
|
Gene symbol:
|
Nefm
|
|
Gene name:
|
neurofilament, medium polypeptide
|
|
Antibody:
|
Anti-Neurofilament M (145 kDa)
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 568
|
|
Assay notes:
|
Modified Carnoy's fixative: 60% ethanol/30% formaldehyde/10% acetic acid
|
|
|
Results
|
Specimen
1M/N:
embryonic day 15.5
(more  )
Note: Triple labeled: red - Nefm; green - Cdkn1b (assay MGI:5547532); blue - Dll3 (assay MGI:5547510).
Specimen
1M/N:
(close  )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Carnoy's Fixative
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: red - Nefm; green - Cdkn1b (assay MGI:5547532); blue - Dll3 (assay MGI:5547510).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlear ganglion |
Present |
Regionally restricted |
1M
,
1N
|
Expression was detected in spiral ganglion neurons, whose processes terminated near the presumptive hair cells.
|
|
Analysis Tools
