Pecam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:90277 Yao HH, et al., Follistatin operates downstream of Wnt4 in mammalian ovary organogenesis. Dev Dyn. 2004 Jun;230(2):210-5
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5528681
Gene symbol:	Pecam1
Gene name:	platelet/endothelial cell adhesion molecule 1

Antibody:	Anti-Pecam-1
Detection system:	Secondary antibody coupled to Cy3

Results

3A WT XX ©

Specimen 3A WT XX: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: germ cell of ovary	Present		3A WT XX

3A Fst-/- XX ©

Specimen 3A Fst-/- XX: embryonic day 12.5; Fst^tm1Zuk/Fst^tm1Zuk (more

)

Structure	Level	Pattern	Image	Note
TS20: ovary	Present	Regionally restricted	3A Fst-/- XX	Expression was detected in the coelomic vessel.
TS20: germ cell of ovary	Present		3A Fst-/- XX

3A Wnt4-/- XX ©

Specimen 3A Wnt4-/- XX: embryonic day 12.5; Wnt4^tm1Amc/Wnt4^tm1Amc (more

)

Structure	Level	Pattern	Image	Note
TS20: ovary	Present	Regionally restricted	3A Wnt4-/- XX	Expression was detected in the coelomic vessel.
TS20: germ cell of ovary	Present		3A Wnt4-/- XX

3A WT XY ©

Specimen 3A WT XY: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: testis	Present	Regionally restricted	3A WT XY	Expression was detected in the coelomic vessel.
TS20: germ cell of testis	Present		3A WT XY

3B WT XX ©

Specimen 3B WT XX: embryonic day 16.5 (more

)
Note: Double labeled: red - Pecam1; blue - TUNEL.

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		3B WT XX	About 30% of the germ cells undergo apoptosis. Apoptosis is restricted to the medullary region.

3B Fst-/- XX ©

Specimen 3B Fst-/- XX: embryonic day 16.5; Fst^tm1Zuk/Fst^tm1Zuk (more

)
Note: Double labeled: red - Pecam1; blue - TUNEL.

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		3B Fst-/- XX	More than 90% of germ cells undergo apoptosis in the mutant. Apoptosis takes place throughout the gonad in the mutant.

3B Wnt4-/- XX ©

Specimen 3B Wnt4-/- XX: embryonic day 16.5; Wnt4^tm1Amc/Wnt4^tm1Amc (more

)
Note: Double labeled: red - Pecam1; blue - TUNEL.

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		3B Wnt4-/- XX	More than 90% of germ cells undergo apoptosis in the mutant. Apoptosis takes place throughout the gonad in the mutant.

3C WT XX ©

Specimen 3C WT XX: embryonic day 16.5 (more

)
Note: Double labeled: red - Pecam1; green - Sycp1 (assay MGI:5528682) and Sycp3 (assay MGI:5528683).

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		3C WT XX

3C Fst-/- XX ©

Specimen 3C Fst-/- XX: embryonic day 16.5; Fst^tm1Zuk/Fst^tm1Zuk (more

)
Note: Double labeled: red - Pecam1; green - Sycp1 (assay MGI:5528682) and Sycp3 (assay MGI:5528683).

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		3C Fst-/- XX

3C Wnt4-/- XX ©

Specimen 3C Wnt4-/- XX: embryonic day 16.5; Wnt4^tm1Amc/Wnt4^tm1Amc (more

)
Note: Double labeled: red - Pecam1; green - Sycp1 (assay MGI:5528682) and Sycp3 (assay MGI:5528683).

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		3C Wnt4-/- XX

4A WT XX ©

Specimen 4A WT XX: embryonic day 18.5 (more

)
Note: Double labeled: red - Pecam1; green - Laminin.

Structure	Level	Pattern	Image	Note
TS26: germ cell of ovary	Present		4A WT XX

4A Fst-/- XX ©

Specimen 4A Fst-/- XX: embryonic day 18.5; Fst^tm1Zuk/Fst^tm1Zuk (more

)
Note: Double labeled: red - Pecam1; green - Laminin.

Structure	Level	Pattern	Image	Note
TS26: germ cell of ovary	Present	Single cells	4A Fst-/- XX	There was almost a complete loss of germ cells near birth in the mutant.

4B WT XX ©

Specimen 4B WT XX: embryonic day 18.5 (more

)
Note: Double labeled: red - Pecam1; green - Sycp1 (assay MGI:5528682) and Sycp3 (assay MGI:5528683).

Structure	Level	Pattern	Image	Note
TS26: germ cell of ovary	Present		4B WT XX

4B Fst-/- XX ©

Specimen 4B Fst-/- XX: embryonic day 18.5; Fst^tm1Zuk/Fst^tm1Zuk (more

)
Note: Double labeled: red - Pecam1; green - Sycp1 (assay MGI:5528682) and Sycp3 (assay MGI:5528683).

Structure	Level	Pattern	Image	Note
TS26: germ cell of ovary	Present	Single cells	4B Fst-/- XX	There was almost a complete loss of germ cells near birth in the mutant.

4C WT XX ©

Specimen 4C WT XX: embryonic day 18.5 (more

)
Note: Double labeled: red - Pecam1; green - Laminin.

Structure	Level	Pattern	Image	Note
TS26: germ cell of ovary	Present		4C WT XX

4C Fst-/- XX ©

Specimen 4C Fst-/- XX: embryonic day 18.5; Fst^tm1Zuk/Fst^tm1Zuk (more

)
Note: Double labeled: red - Pecam1; green - Laminin.

Structure	Level	Pattern	Image	Note
TS26: germ cell of ovary	Present	Single cells	4C Fst-/- XX	There was almost a complete loss of germ cells near birth in the mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24