Probe:	Rdh10 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: hindbrain floor plate	Weak
TS17: midbrain	Weak

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	2A	Expression was detected in the meninges.
TS20: choroid plexus	Present		2A

Structure	Level	Pattern	Image	Note
TS20: diencephalon ventricular layer	Present	Regionally restricted	2B	Expression was detected in the ventricular neuroepithelium around the 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Weak		2C

Structure	Level	Pattern	Image	Note
TS22: brain meninges	Strong		2D

Structure	Level	Pattern	Image	Note
TS24: corpus striatum	Present		2F

Structure	Level	Pattern	Image	Note
TS26: brain meninges	Present		2G
TS26: diencephalon ventricular layer	Present	Regionally restricted	2G	Expression was detected in the ventricular neuroepithelium around the 3rd ventricle.
TS26: choroid plexus	Strong		2G

Structure	Level	Pattern	Image	Note
TS26: corpus striatum	Present		2H
TS26: choroid plexus	Strong		2H

Structure	Level	Pattern	Image	Note
TS26: brain meninges	Present	Regionally restricted	2I	Expression in the meninges tended to decrease as development proceeded, although it remained high in some regions (e.g., in the ventral meninges surrounding the hippocampus.
TS26: choroid plexus	Strong		2I

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	3A	Expression was restricted to the dorsal portion of the optic vesicle neuroepithelium.

Structure	Level	Pattern	Image	Note
TS17: optic cup outer layer	Present		3B	Expression was detected in the prospective retinal pigmented epithelium.
TS17: optic cup inner layer	Present	Regionally restricted	3B	Expression was detected in part of the developing neural retina.

Structure	Level	Pattern	Image	Note
TS20: pigmented retinal epithelium	Strong		3C	Staining was intensified in the retinal pigmented epithelium.
TS20: neural retinal epithelium	Present	Patchy	3C	Patchy expression was regionalized toward the periphery of the retina, and was most intense where the rim between neural and pigment epithelium was apparent.
TS20: eye	Present	Regionally restricted	3C	Expression was detected in the surface ectoderm and mesenchyme underlying the developing palpebral folds.

Structure	Level	Pattern	Image	Note
TS20: pigmented retinal epithelium	Strong		3D
TS20: neural retinal epithelium	Present	Patchy	3D
TS20: lens vesicle	Absent		3D
TS20: eye	Present	Regionally restricted	3D	Expression was detected in the surface ectoderm and mesenchyme underlying the developing palpebral folds.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	3E	Expression extended all around the retina rim.
TS22: pigmented retinal epithelium	Strong		3E
TS22: eyelid	Present	Regionally restricted	3E	Selective labeling was observed in the vicinity of the palpebral folds.

Structure	Level	Pattern	Image	Note
TS24: pigmented retinal epithelium	Strong

Structure	Level	Pattern	Image	Note
TS26: pigmented retinal epithelium	Strong		3F
TS26: ciliary body	Present	Regionally restricted	3F	Expression was detected in the ciliary body, especially at the level of the pseudostratified inner ciliary epithelium.
TS26: neural retina	Present	Regionally restricted	3F	Expression was detected at lower levels along the adjacent marginal region of the neural retina.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	4A	Expression was detected in the rostromedial region of the otocyst.

Structure	Level	Pattern	Image	Note
TS17: endolymphatic appendage	Present		4B

Structure	Level	Pattern	Image	Note
TS20: endolymphatic appendage	Strong		4C
TS20: endolymphatic sac	Strong		4C

Structure	Level	Pattern	Image	Note
TS20: endolymphatic appendage	Strong		4D	Expression extended up to the origin of the duct close to the utriculus.
TS20: inner ear epithelium	Weak	Regionally restricted	4D	No signal was observed in the sensory epithelium, but a faint labeling was present on the apical surface of the nonsensory epithelium.

Structure	Level	Pattern	Image	Note
TS22: endolymphatic duct	Strong		4E
TS22: endolymphatic sac	Strong		4E
TS22: middle ear epithelium	Present		4E
TS22: middle ear mesenchyme	Present		4E
TS22: cochlea	Present		4E
TS22: inner ear vestibular component	Present		4E

Structure	Level	Pattern	Image	Note
TS22: external auditory meatus	Present		4F

Structure	Level	Pattern	Image	Note
TS22: cochlea	Present	Regionally restricted	4G	Expression was detected in the presumptive stria vascularis and a faint labeling was present in the sensory region.

Structure	Level	Pattern	Image	Note
TS22: inner ear vestibular component	Weak	Patchy	4H	Expression was detected in nonsensory regions that may correspond to the dark cells in the semicircular canals. No signal was detected in the sensory region.

Structure	Level	Pattern	Image	Note
TS24: external auditory meatus	Present
TS24: middle ear	Present

Structure	Level	Pattern	Image	Note
TS26: middle ear	Present	Regionally restricted	4I	Expression was detected in the epithelium of the middle ear cavity.
TS26: external auditory meatus	Present		4I
TS26: tympanic membrane	Present		4I

Structure	Level	Pattern	Image	Note
TS26: endolymphatic duct epithelium	Present		4J
TS26: endolymphatic sac epithelium	Present		4J
TS26: cochlea	Weak		4J	Faint expression was detected in the cochlear canal (stria vascularis).
TS26: inner ear vestibular component	Weak		4J	Faint expression was detected in the vestibular canals and vestibular organs.

Structure	Level	Pattern	Image	Note
TS17: nasal pit epithelium	Present	Regionally restricted	5A	Expression was localized along the lateral region and margin of the nasal pit epithelium.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present	Regionally restricted	5C , 5B	Expression was detected in the nasal epithelium lining the lateral side of the lumen of the primitive nasal cavity. The signal was mainly regionalized and patchy among cells in the recesses of the olfactory epithelium.
TS20: latero-nasal process mesenchyme	Present	Regionally restricted	5B	Expression was detected in distal mesenchymal cells of the lateral nasal process.
TS20: nasal mesenchyme	Present	Regionally restricted	5B	Expression was detected in distal mesenchymal cells of the lateral nasal process, but no expression was detected in the mesenchyme surrounding the nasal and olfactory epithelium proper.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	5D	Expression intensified and was concentrated toward the distal recesses of the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	5E	Expression intensified and was concentrated toward the distal recesses of the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS26: olfactory epithelium	Weak	Single cells	5F , 5G	Expression had almost receded by E18.5, although weak signals were still detectable in a few olfactory epithelial cells.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Weak	Regionally restricted	5H	Faint expression was detected in the external layer.

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Regionally restricted	5I	Expression was detected in the snout region as rings of signal located at the level of the whisker follicles.

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted		Expression was detected in rings surrounding the whisker follicles.

Structure	Level	Pattern	Image	Note
TS26: vibrissa follicle	Present	Regionally restricted	5J	Expression was detected at the level of the basis of the hair follicles.
TS26: skin	Present	Regionally restricted	5J	Expression was detected at the border of the dermis and subcutis outside the hair follicles.

Structure	Level	Pattern	Image	Note
TS26: skin	Present	Regionally restricted	5K	Expression was detected at the border of the dermis and subcutis outside the hair follicles.
TS26: vibrissa follicle	Present	Regionally restricted	5K	Expression was detected at the level of the basis of the hair follicles.

Structure	Level	Pattern	Image	Note
TS26: vibrissa follicle	Present	Regionally restricted	5L	Expression was detected in the hair bulbs at the level of the internal rooth sheath.