Reporter:	lacZ
	Direct Detection
Assay notes:	Fixative for whole mounts and paraffin embedded specimens the was 2% paraformaldehyde/0.5% glutaraldehyde. For older animals ages E17, P0, P6, 1 month, and 3 months, brains were dissected and fresh frozen and postfixed in the same fixative.

Structure	Level	Pattern	Image	Note
TS25: brain	Present		2E

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2G

Structure	Level	Pattern	Image	Note
TS14: future midbrain roof plate	Present	Regionally restricted	3A	Expression is in the roof of the anterior mesencephalon.
TS14: optic vesicle	Present		3A
TS14: somite	Present		3A
TS14: embryo	Present	Regionally restricted	3A	Expression is at the caudal tip.
TS14: future hindbrain	Weak		3A
TS14: prosencephalon	Absent		3A
TS14: visceral organ system	Absent		3A

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted	3B	Expression is in the lateral ganglionic eminence, primarily along the neuroepithelium. Absent in the medial ganglionic eminence and neocortex.
TS18: cranial nerve	Moderate	Regionally restricted	3B	Expression is in the semilunar ganglion of cranial nerve V.
TS18: midbrain	Present	Regionally restricted	3B	Expression is in the pretectal area, the anterior ventral tegmentum, and the tectum.
TS18: metencephalon	Moderate	Regionally restricted	3B	Expression is in the pons and cerebellar plate.
TS18: acoustic ganglion	Present		3B
TS18: spinal ganglion	Present		3B
TS18: trigeminal ganglion	Present		3B
TS18: vagus ganglion	Present		3B
TS18: future spinal cord	Present	Regionally restricted	3B	Expression is ventral.
TS18: limb	Absent		3B
TS18: visceral organ system	Absent		3B

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Strong		3C
TS21: trigeminal ganglion	Strong		3C
TS21: vagus ganglion	Strong		3C
TS21: forelimb	Present	Regionally restricted	3C	Expression is in isolated patches.
TS21: hindlimb	Present	Regionally restricted	3C	Expression is in isolated patches.
TS21: cardiovascular system	Absent		3C
TS21: visceral organ system	Absent		3C

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Strong		3D
TS23: spinal cord	Present	Regionally restricted	3D	Expression is ventral.
TS23: forelimb	Present		3D
TS23: hindlimb	Present		3D
TS23: spinal ganglion	Present		3D

Structure	Level	Pattern	Image	Note
TS14: cardiovascular system	Absent		4A
TS14: prosencephalon	Absent		4A
TS14: visceral organ system	Absent		4A

Structure	Level	Pattern	Image	Note
TS14: cardiovascular system	Absent		4B
TS14: prosencephalon	Absent		4B
TS14: visceral organ system	Absent		4B

Structure	Level	Pattern	Image	Note
TS14: future midbrain roof plate	Present	Regionally restricted	4C	Expression is in the roof of the anterior mesencephalon.
TS14: cardiovascular system	Absent		4C
TS14: prosencephalon	Absent		4C
TS14: visceral organ system	Absent		4C

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Weak		4D

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted	5A	Expression is in the lateral ganglionic eminence, primarily along the neuroepithelium. Absent in the medial ganglionic eminence and neocortex.
TS18: future spinal cord	Present	Regionally restricted	5A	Expression is ventral.
TS18: spinal ganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted	5B	Expression is in the lateral ganglionic eminence, primarily along the neuroepithelium. Absent in the medial ganglionic eminence and neocortex.
TS18: midbrain	Present	Regionally restricted	5B	Expression is in the pretectal area, the anterior ventral tegmentum, and the tectum.
TS18: future spinal cord	Present	Regionally restricted	5B	Expression is ventral.
TS18: spinal ganglion	Present		5B

Structure	Level	Pattern	Image	Note
TS18: eye	Strong	Graded	5C	There is a temporal to nasal gradient of expression in the optic cup.
TS18: diencephalon	Weak	Regionally restricted	5C	Expression is dorsally in the primordial thalamus.
TS18: midbrain	Present	Regionally restricted	5C	Expression is in the pretectal area, the anterior ventral tegmentum, and the tectum.
TS18: future spinal cord	Present	Regionally restricted	5C	Expression is ventral.
TS18: spinal ganglion	Present		5C

Structure	Level	Pattern	Image	Note
TS18: metencephalon	Moderate	Regionally restricted	5D	Expression is in the pons and cerebellar plate.
TS18: acoustic ganglion	Present		5D
TS18: spinal ganglion	Present		5D
TS18: trigeminal ganglion	Present		5D
TS18: vagus ganglion	Present		5D
TS18: future spinal cord	Present	Regionally restricted	5D	Expression is ventral.
TS18: limb	Absent		5D
TS18: visceral organ system	Absent		5D

Structure	Level	Pattern	Image	Note
TS18: metencephalon	Moderate	Regionally restricted	5E	Expression is in the pons and cerebellar plate.
TS18: acoustic ganglion	Present		5E
TS18: spinal ganglion	Present		5E
TS18: trigeminal ganglion	Present		5E
TS18: vagus ganglion	Present		5E
TS18: future spinal cord	Present	Regionally restricted	5E	Expression is ventral.
TS18: limb	Absent		5E
TS18: visceral organ system	Absent		5E

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted	5F	Expression is in the lateral ganglionic eminence, primarily along the neuroepithelium. Absent in the medial ganglionic eminence and neocortex.
TS18: cranial nerve	Moderate	Regionally restricted	5F	Expression is in the semilunar ganglion of cranial nerve V.
TS18: midbrain	Present	Regionally restricted	5F	Expression is in the pretectal area, the anterior ventral tegmentum, and the tectum.
TS18: metencephalon	Moderate	Regionally restricted	5F	Expression is in the pons and cerebellar plate.
TS18: acoustic ganglion	Present		5F
TS18: spinal ganglion	Present		5F
TS18: trigeminal ganglion	Present		5F
TS18: vagus ganglion	Present		5F
TS18: future spinal cord	Present	Regionally restricted	5F	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS18: eye	Strong	Graded	5G	There is a temporal to nasal gradient of expression in the optic cup.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Strong		6A

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Strong		6B
TS21: olfactory lobe	Present		6B

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Strong		6C
TS21: telencephalon	Present	Regionally restricted	6C	Expression is in the hippocampus.
TS21: lateral ganglionic eminence	Present		6C
TS21: thalamus	Absent		6C

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Strong		6D
TS21: telencephalon	Present	Regionally restricted	6D	Expression is in the hippocampus. Absent in amygdala.
TS21: lateral ganglionic eminence	Present		6D
TS21: midbrain	Strong	Regionally restricted	6D	Expression is in the pretectum and superior colliculus.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Strong		6E
TS21: trigeminal ganglion	Strong		6E

Structure	Level	Pattern	Image	Note
TS21: hypoglossal nerve	Strong		6F

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Strong		7A

Structure	Level	Pattern	Image	Note
TS23: lateral ganglionic eminence	Present		7B
TS23: medial ganglionic eminence	Absent		7B

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		7C

Structure	Level	Pattern	Image	Note
TS23: pretectum	Strong		7D
TS23: tectum	Strong		7D

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Present		7E
TS23: vestibulocochlear nerve	Present		7E

Structure	Level	Pattern	Image	Note
TS23: hypoglossal nerve	Present		7F

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		8A

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		8B

Structure	Level	Pattern	Image	Note
TS23: lateral ganglionic eminence	Present		8C
TS23: medial ganglionic eminence	Absent		8C
TS23: hippocampus	Present		8C
TS23: spinal cord	Present	Regionally restricted	8C	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS23: pretectum	Strong		8D
TS23: tectum	Strong		8D
TS23: glans of male genital tubercle	Strong	Regionally restricted	8D	Expression is in the ventral glans penis.
TS23: epithalamus	Present		8D
TS23: hippocampus	Present		8D
TS23: spinal ganglion	Present		8D
TS23: thalamus	Trace	Regionally restricted	8D	Expression in the thalamic nuclei were either negative or weak.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Strong		9A
TS25: cortical subplate	Present		9A
TS25: main olfactory bulb	Present		9A
TS25: piriform cortex	Present		9A
TS25: eye	Present	Graded	9A	Expression is in a temporonasal gradient.
TS25: neural retina	Present	Regionally restricted	9A	Expression is in the retinal ganglion cell layer.
TS25: telencephalon	Present	Regionally restricted	9A	Expression is in the claustrum.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Strong		9B
TS25: cortical subplate	Present		9B
TS25: piriform cortex	Present		9B
TS25: telencephalon	Present	Regionally restricted	9B	Expression is in the induseum griseum and the claustrum.
TS25: corpus striatum	Weak		9B
TS25: telencephalon septum	Weak		9B

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Strong		9C
TS25: hippocampus	Present	Graded	9C	Expression is in a decreasing gradient from the medial to the lateral areas.
TS25: thalamus	Present	Regionally restricted	9C	Expression is in the nucleus reuniens and several dorsal thalamic nuclear groups.
TS25: epithalamus	Present		9C
TS25: hypothalamus	Weak		9C

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Strong		9D
TS25: hippocampus	Present	Graded	9D	Expression is in a decreasing gradient from the medial to the lateral areas.
TS25: thalamus	Present	Regionally restricted	9D	Expression is in the nucleus reuniens and several dorsal thalamic nuclear groups.
TS25: epithalamus	Present		9D
TS25: hypothalamus	Weak

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Strong		9E
TS25: hippocampus	Present	Graded	9E	Expression is in a decreasing gradient from the medial to the lateral areas.
TS25: pretectum	Weak		9E
TS25: superior colliculus	Weak		9E

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Strong		9F
TS25: pretectum	Weak		9F
TS25: superior colliculus	Weak		9F

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb glomerular layer	Present		10A
TS27: main olfactory bulb mitral cell layer	Present		10A

Structure	Level	Pattern	Image	Note
TS27: indusium griseum	Very strong		10B
TS27: piriform cortex	Strong		10B
TS27: lateral septal complex	Moderate		10B
TS27: insular cortex	Present		10B
TS27: parietal cortex	Present		10B
TS27: basal ganglia	Weak		10B
TS27: cingulate cortex	Weak		10B
TS27: frontal cortex	Weak		10B

Structure	Level	Pattern	Image	Note
TS27: indusium griseum	Very strong		10C
TS27: anterior thalamic group	Present		10C
TS27: intralaminar thalamic group	Present		10C
TS27: lateral thalamic group	Present		10C
TS27: nucleus reuniens	Present		10C
TS27: corpus striatum	Present	Regionally restricted	10C	Absent in the dorsal striatum.
TS27: accumbens nucleus	Present		10C	There is a discrepancy between the text and the table, Fig 10C states expression is present but table 2 indicates absence of expression.
TS27: hypothalamus	Weak		10C
TS27: caudate-putamen	Weak		10C	There is a discrepancy between the text and the table, Fig 10C states expression is present but table 2 indicates absence of expression.
TS27: globus pallidus	Absent		10C

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong		10D
TS27: anterior thalamic group	Present		10D
TS27: intralaminar thalamic group	Present		10D
TS27: lateral thalamic group	Present		10D
TS27: nucleus reuniens	Present		10D
TS27: hypothalamus	Weak		10D
TS27: dentate gyrus	Absent		10D	There is a discrepancy between the text and the table, Fig 10D,E states expression is abent in the dentate gyrus but table 2 indicates expression.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong		10E
TS27: anterior thalamic group	Present		10E
TS27: intralaminar thalamic group	Present		10E
TS27: lateral thalamic group	Present		10E
TS27: nucleus reuniens	Present		10E
TS27: hypothalamus	Weak		10E
TS27: dentate gyrus	Absent		10E	There is a discrepancy between the text and the table, Fig 10D,E states expression is abent in the dentate gyrus but table 2 indicates expression.

Structure	Level	Pattern	Image	Note
TS27: midbrain	Present	Regionally restricted	10F	Some signals were detected around the ventricle, in the central gray region.
TS27: pretectum	Absent		10F
TS27: tectum	Absent		10F

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong		11A
TS28: main olfactory bulb mitral cell layer	Strong		11A

Structure	Level	Pattern	Image	Note
TS28: insular cortex	Strong		11B
TS28: parietal cortex	Strong		11B
TS28: piriform cortex	Strong		11B
TS28: cingulate cortex	Present		11B
TS28: frontal cortex	Present		11B

Structure	Level	Pattern	Image	Note
TS28: insular cortex	Strong		11C
TS28: parietal cortex	Strong		11C
TS28: cingulate cortex	Present		11C
TS28: frontal cortex	Present		11C
TS28: caudate-putamen	Present	Regionally restricted	11C	Expression is in the striatal patches.

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Strong		11D
TS28: hippocampus	Strong		11D
TS28: insular cortex	Strong		11D
TS28: parietal cortex	Strong		11D
TS28: cingulate cortex	Present		11D
TS28: frontal cortex	Present		11D
TS28: dentate gyrus	Present	Regionally restricted	11D	Expression is as a thin line.
TS28: thalamic nucleus	Present	Regionally restricted	11D	Expression is in various nuclei.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Strong		11E
TS28: cortical layer III	Strong		11E
TS28: cortical layer V	Strong		11E
TS28: hippocampus	Strong		11E
TS28: cortical layer I	Present		11E
TS28: cortical layer IV	Present		11E
TS28: cortical layer VI	Present		11E
TS28: dentate gyrus	Present	Regionally restricted	11E	Expression is as a thin line.
TS28: thalamic nucleus	Present	Regionally restricted	11E	Expression is in various nuclei.
TS28: ventral posterior lateral thalamic nucleus	Absent		11E
TS28: ventral posterior medial thalamic nucleus	Absent		11E

Structure	Level	Pattern	Image	Note
TS28: pretectum	Weak		11F
TS28: substantia nigra	Weak		11F
TS28: tectum	Weak		11F
TS28: ventral tegmental area	Weak		11F
TS28: midbrain	Weak		11F	Expression is in ventral dopaminergic nuclei.

Structure	Level	Pattern	Image	Note
TS28: olfactory lobe	Present		12A	Strongest staining of the anterior nucleus of the olfactory lobes was found in the lateral and ventral lobes.
TS28: claustrum	Strong		12A

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Very strong		12B
TS28: cerebral cortex	Strong		12B
TS28: endopiriform nucleus	Present		12B
TS28: piriform cortex	Present		12B
TS28: olfactory tract	Absent		12B
TS28: olfactory tubercle	Absent		12B

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Very strong		12C
TS28: cerebral cortex	Strong		12C
TS28: hypothalamic nucleus	Strong		12C
TS28: caudate-putamen	Moderate		12C	Stronger signals were found in the striatal patches.
TS28: habenula	Moderate		12C
TS28: globus pallidus	Trace		12C	Little or no staining.

Structure	Level	Pattern	Image	Note
TS28: hypothalamic nucleus	Strong		12D
TS28: habenula	Moderate		12D
TS28: amygdala	Present		12D
TS28: dentate gyrus granule cell layer	Present		12D
TS28: hypothalamus	Present		12D
TS28: nucleus reuniens	Present		12D
TS28: zona incerta	Present		12D
TS28: thalamic nucleus	Present	Regionally restricted	12D	Expression is in the outer nuclei of the thalamus and in specific dorsal thalamic nuclei.
TS28: dentate gyrus	Weak		12D
TS28: cerebellar peduncle	Absent		12D

Structure	Level	Pattern	Image	Note
TS28: cortical amygdaloid nucleus	Present		12E
TS28: cerebellar peduncle	Absent		12E

Structure	Level	Pattern	Image	Note
TS28: interpeduncular nucleus	Present		12F
TS28: substantia nigra	Present		12F
TS28: ventral tegmental area	Present		12F
TS28: periaqueductal grey	Weak		12F
TS28: pretectum	Weak		12F
TS28: superior colliculus	Weak		12F
TS28: inferior colliculus	Absent		12F
TS28: red nucleus	Absent		12F

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Very strong		13A
TS28: cerebral cortex	Strong		13A
TS28: amygdala	Present		13A
TS28: dentate gyrus granule cell layer	Present		13A
TS28: hypothalamus	Present		13A
TS28: cerebellum Purkinje cell layer	Present	Scattered	13A
TS28: dentate gyrus	Weak		13A

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Very strong		13B
TS28: cerebral cortex	Strong		13B
TS28: amygdala	Present		13B
TS28: dentate gyrus granule cell layer	Present		13B
TS28: endopiriform nucleus	Present		13B
TS28: hypothalamus	Present		13B
TS28: piriform cortex	Present		13B
TS28: dentate gyrus	Weak		13B

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Very strong		13C
TS28: cerebral cortex	Strong		13C
TS28: caudate-putamen	Moderate		13C	Stronger signals were found in the striatal patches.
TS28: amygdala	Present		13C
TS28: dentate gyrus granule cell layer	Present		13C
TS28: hypothalamus	Present		13C
TS28: dentate gyrus	Weak		13C
TS28: pons	Weak		13C
TS28: globus pallidus	Absent		13C
TS28: olfactory tract	Absent		13C
TS28: olfactory tubercle	Absent		13C

Structure	Level	Pattern	Image	Note
TS28: olfactory cortex	Strong	Regionally restricted	13D	Expression is in the accessory nucleus.
TS28: hypothalamic nucleus	Strong		13D
TS28: habenula	Moderate		13D
TS28: amygdala	Present		13D
TS28: dentate gyrus granule cell layer	Present		13D
TS28: hypothalamus	Present		13D
TS28: substantia nigra	Present		13D
TS28: ventral tegmental area	Present		13D
TS28: dentate gyrus	Weak		13D
TS28: dorsal thalamus	Weak		13D
TS28: periaqueductal grey	Weak		13D
TS28: pons	Weak		13D
TS28: pretectum	Weak		13D
TS28: superior colliculus	Weak		13D
TS28: prethalamus	Weak		13D	Weaker than seen in the dorsal thalamus.
TS28: inferior colliculus	Absent		13D

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb external plexiform layer	Strong		14A
TS28: main olfactory bulb glomerular layer	Strong		14A
TS28: main olfactory bulb granule cell layer	Strong		14A
TS28: main olfactory bulb internal plexiform layer	Strong		14A
TS28: main olfactory bulb mitral cell layer	Strong		14A

Structure	Level	Pattern	Image	Note
TS28: basolateral amygdaloid nucleus	Strong		14B
TS28: basomedial amygdaloid nucleus	Strong		14B
TS28: central amygdaloid nucleus	Strong		14B
TS28: lateral amygdaloid nucleus	Strong		14B

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Very strong		14C
TS28: hippocampus stratum oriens	Strong		14C	Strongest just dorsal to the pyramidal cells.
TS28: hippocampus molecular layer	Strong		14C

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present	Regionally restricted		Expression is in the pretectum and superior colliculus, absent in the inferior colliculus.
TS14: future spinal cord	Moderate	Regionally restricted		Expression is in the distal spinal cord, absent in proximal spinal cord.
TS14: future diencephalon	Absent			Absent in the thalamus.
TS14: prosencephalon	Absent			Absent in cerebral cortex.

Structure	Level	Pattern	Image	Note
TS18: cranial nerve	Moderate
TS18: retina	Moderate
TS18: midbrain	Moderate	Regionally restricted		Expression is in the pretectum, superior collicus, and is weak in the ventral midbrain.
TS18: future spinal cord	Moderate	Regionally restricted		Expression is in the distal spinal cord, absent in proximal spinal cord.
TS18: hindbrain	Weak	Regionally restricted		Expression is in the pons and cerebellum.
TS18: diencephalon	Weak	Regionally restricted		Expression is in the epithalamus and hypothalamus, absent in thalamus.
TS18: telencephalon	Weak	Regionally restricted		Expression is in the lateral ganglionic eminence, absent in the medial ganglionic eminence and neocortex.

Structure	Level	Pattern	Image	Note
TS21: hypoglossal nerve	Moderate
TS21: neocortex	Moderate
TS21: olfactory lobe	Moderate
TS21: retina	Moderate
TS21: trigeminal nerve	Moderate
TS21: vestibulocochlear nerve	Moderate
TS21: midbrain	Moderate	Regionally restricted		Expression is in the pretectum, superior colliculus, and the ventral midbrain, and is absent in the inferior colliculus.
TS21: spinal cord	Moderate	Regionally restricted		Expression is in the distal spinal cord, weak in proximal spinal cord.
TS21: forebrain	Weak	Regionally restricted		Expression is in hippocampus.
TS21: lateral ganglionic eminence	Weak
TS21: medial ganglionic eminence	Absent
TS21: cerebellum	Weak
TS21: epithalamus	Weak
TS21: hypothalamus	Weak
TS21: pons	Weak
TS21: thalamus	Absent

Structure	Level	Pattern	Image	Note
TS23: hippocampus	Moderate
TS23: hypoglossal nerve	Moderate
TS23: main olfactory bulb	Moderate
TS23: neocortex	Moderate
TS23: pretectum	Moderate
TS23: retina	Moderate
TS23: superior colliculus	Moderate
TS23: trigeminal nerve	Moderate
TS23: vestibulocochlear nerve	Moderate
TS23: midbrain	Weak	Regionally restricted		Expression is in the ventral midbrain.
TS23: lateral ganglionic eminence	Weak
TS23: medial ganglionic eminence	Absent
TS23: spinal cord	Moderate	Regionally restricted		Expression is in the distal spinal cord, weak in proximal spinal cord.
TS23: cerebellum	Weak
TS23: epithalamus	Weak
TS23: hypothalamus	Weak
TS23: pons	Weak
TS23: thalamus	Trace			The text states that expression in the thalamic nuclei were either negative or weak. The table indicates expression is absent in the thalamus.
TS23: inferior colliculus	Absent

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Strong	Regionally restricted		Expression is in the induseum griseum, weak in claustrum and lateral septum and absent in the globus pallidus and medial septum.
TS25: hippocampus	Strong
TS25: main olfactory bulb	Moderate
TS25: neocortex	Moderate
TS25: retina	Moderate
TS25: spinal cord	Moderate
TS25: cerebral cortex	Moderate	Regionally restricted		Strong in piriform cortex, moderate in cingulate, entorhinal, insular, and parietal, weak in frontal,
TS25: caudate-putamen	Weak
TS25: cerebellum	Weak
TS25: epithalamus	Weak
TS25: hypothalamus	Weak
TS25: pons	Weak
TS25: pretectum	Weak
TS25: superior colliculus	Weak
TS25: thalamus	Weak
TS25: midbrain	Weak	Regionally restricted		Expression is in the ventral midbrain.
TS25: inferior colliculus	Absent

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA1	Strong
TS27: hippocampus CA2	Strong
TS27: hippocampus CA3	Strong
TS27: indusium griseum	Strong
TS27: hippocampus pyramidal cell layer	Moderate
TS27: accessory olfactory bulb granule cell layer	Weak
TS27: amygdalohippocampal area	Weak
TS27: amygdalopiriform transition area	Weak
TS27: anterior amygdaloid area	Weak
TS27: anterior thalamic group	Weak
TS27: basolateral amygdaloid nucleus	Weak
TS27: basomedial amygdaloid nucleus	Weak
TS27: bed nucleus of stria terminalis	Weak
TS27: brain subventricular zone	Weak
TS27: central amygdaloid nucleus	Weak
TS27: cerebellum Purkinje cell layer	Weak
TS27: cingulate cortex	Weak
TS27: claustrum	Weak
TS27: cortical layer II	Weak
TS27: cortical layer III	Weak
TS27: cortical layer IV	Weak
TS27: cortical layer V	Weak
TS27: cortical layer VI	Weak
TS27: endopiriform nucleus	Weak
TS27: fasciola cinereum	Weak
TS27: hippocampus stratum oriens	Weak
TS27: insular cortex	Weak
TS27: intralaminar thalamic group	Weak
TS27: lateral amygdaloid nucleus	Weak
TS27: lateral hypothalamic area	Weak
TS27: lateral septal complex	Weak
TS27: lateral thalamic group	Weak
TS27: main olfactory bulb glomerular layer	Weak
TS27: main olfactory bulb granule cell layer	Weak
TS27: main olfactory bulb internal plexiform layer	Weak
TS27: main olfactory bulb mitral cell layer	Weak
TS27: medial amygdaloid nucleus	Weak
TS27: nucleus of diagonal band	Weak
TS27: nucleus reuniens	Weak
TS27: pallidum	Weak
TS27: piriform cortex	Weak
TS27: primary motor cortex	Weak
TS27: primary somatosensory cortex	Weak
TS27: secondary somatosensory cortex	Weak
TS27: substantia nigra	Weak
TS27: taenia tecta	Weak
TS27: ventral tegmental area	Weak
TS27: zona incerta	Weak
TS27: dentate gyrus polymorphic layer	Weak			There is a discrepancy between the text and the table, Fig 10D,E states expression is abent in the dentate gyrus but table 2 indicates expression.
TS27: anterior olfactory nucleus	Weak	Regionally restricted		Weak in dorsal and lateral, absent in external, medial, and ventral anterior olfactory nuclei.
TS27: anterior commissure	Absent
TS27: arcuate nucleus	Absent
TS27: barrel cortex	Absent
TS27: cerebellum external granule cell layer	Absent
TS27: cerebellum molecular layer	Absent
TS27: corpus callosum	Absent
TS27: cortical amygdaloid nucleus	Absent
TS27: cortical layer I	Absent
TS27: dentate gyrus granule cell layer	Absent
TS27: dentate gyrus molecular layer	Absent
TS27: dorsal lateral geniculate nucleus	Absent
TS27: dorsal raphe nucleus	Absent
TS27: dorsomedial hypothalamic nucleus	Absent
TS27: edinger-westphal nucleus	Absent
TS27: fimbria hippocampus	Absent
TS27: globus pallidus	Absent
TS27: hippocampus molecular layer	Absent
TS27: hippocampus stratum lacunosum	Absent
TS27: hippocampus stratum lucidum	Absent
TS27: interpeduncular nucleus	Absent
TS27: lateral habenular nucleus	Absent
TS27: lateral mammillary nucleus	Absent
TS27: lateral preoptic area	Absent
TS27: main olfactory bulb external plexiform layer	Absent
TS27: main olfactory bulb olfactory nerve layer	Absent
TS27: medial geniculate nucleus	Absent
TS27: medial habenular nucleus	Absent
TS27: medial mammillary nucleus	Absent
TS27: medial preoptic nucleus	Absent
TS27: nucleus of lateral olfactory tract	Absent
TS27: paraventricular hypothalamic nucleus	Absent
TS27: periaqueductal grey	Absent
TS27: pontine nucleus	Absent
TS27: pontine reticular formation	Absent
TS27: posterolateral cortical amygdaloid nucleus	Absent
TS27: posteromedial cortical amygdaloid nucleus	Absent
TS27: red nucleus	Absent
TS27: reticular thalamic nucleus	Absent
TS27: septofimbrial nucleus	Absent
TS27: septohippocampal nucleus	Absent
TS27: superior colliculus	Absent
TS27: suprachiasmatic nucleus	Absent
TS27: supramammillary nucleus	Absent
TS27: supraoptic nucleus	Absent
TS27: ventral lateral geniculate nucleus	Absent
TS27: ventral posterior thalamic group	Absent
TS27: ventromedial hypothalamic nucleus	Absent
TS27: accumbens nucleus	Absent			There is a discrepancy between the text and the table, Fig 10C states expression is present but table 2 indicates absence of expression.
TS27: caudate-putamen	Absent			There is a discrepancy between the text and the table, Fig 10C states expression is present but table 2 indicates absence of expression.

Structure	Level	Pattern	Image	Note
TS28: fasciola cinereum	Strong
TS28: hippocampus CA1	Strong
TS28: hippocampus CA2	Strong
TS28: hippocampus CA3	Strong
TS28: hippocampus pyramidal cell layer	Strong
TS28: indusium griseum	Strong
TS28: piriform cortex	Strong
TS28: barrel cortex	Moderate
TS28: bed nucleus of stria terminalis	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: cortical layer IV	Moderate
TS28: cortical layer V	Moderate
TS28: cortical layer VI	Moderate
TS28: dentate gyrus granule cell layer	Moderate
TS28: dentate gyrus molecular layer	Moderate
TS28: dentate gyrus polymorphic layer	Moderate
TS28: endopiriform nucleus	Moderate
TS28: hippocampus molecular layer	Moderate
TS28: hippocampus stratum lacunosum	Moderate
TS28: hippocampus stratum lucidum	Moderate
TS28: hippocampus stratum oriens	Moderate
TS28: insular cortex	Moderate
TS28: lateral septal complex	Moderate
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb mitral cell layer	Moderate
TS28: nucleus of diagonal band	Moderate
TS28: nucleus reuniens	Moderate
TS28: pallidum	Moderate
TS28: primary motor cortex	Moderate
TS28: primary somatosensory cortex	Moderate
TS28: secondary somatosensory cortex	Moderate
TS28: substantia nigra	Moderate
TS28: taenia tecta	Moderate
TS28: ventral tegmental area	Moderate
TS28: accessory olfactory bulb granule cell layer	Weak
TS28: amygdalohippocampal area	Weak
TS28: amygdalopiriform transition area	Weak
TS28: anterior amygdaloid area	Weak
TS28: anterior dorsal thalamic nucleus	Weak
TS28: anterior olfactory nucleus	Weak
TS28: basolateral amygdaloid nucleus	Weak
TS28: basomedial amygdaloid nucleus	Weak
TS28: brain subventricular zone	Weak
TS28: caudate-putamen	Weak
TS28: central amygdaloid nucleus	Weak
TS28: cerebellum Purkinje cell layer	Weak
TS28: cingulate cortex	Weak
TS28: claustrum	Weak
TS28: cortical layer I	Weak
TS28: dorsal lateral geniculate nucleus	Weak
TS28: dorsal raphe nucleus	Weak
TS28: edinger-westphal nucleus	Weak
TS28: interpeduncular nucleus	Weak
TS28: intralaminar thalamic group	Weak
TS28: lateral amygdaloid nucleus	Weak
TS28: lateral habenular nucleus	Weak
TS28: lateral hypothalamic area	Weak
TS28: lateral mammillary nucleus	Weak
TS28: lateral thalamic group	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: main olfactory bulb internal plexiform layer	Weak
TS28: main olfactory bulb olfactory nerve layer	Weak
TS28: medial amygdaloid nucleus	Weak
TS28: medial septal complex	Weak
TS28: paracentral nucleus	Weak
TS28: paraventricular thalamic nucleus	Weak
TS28: periaqueductal grey	Weak
TS28: pontine reticular formation	Weak
TS28: posterior amygdaloid nucleus	Weak
TS28: posterolateral cortical amygdaloid nucleus	Weak
TS28: posteromedial cortical amygdaloid nucleus	Weak
TS28: septofimbrial nucleus	Weak
TS28: septohippocampal nucleus	Weak
TS28: superior colliculus	Weak
TS28: ventral lateral geniculate nucleus	Weak
TS28: zona incerta	Weak
TS28: accumbens nucleus	Absent
TS28: anterior commissure	Absent
TS28: anterior ventral thalamic nucleus	Absent
TS28: arcuate nucleus	Absent
TS28: cerebellum external granule cell layer	Absent
TS28: cerebellum molecular layer	Absent
TS28: corpus callosum	Absent
TS28: dorsomedial hypothalamic nucleus	Absent
TS28: fimbria hippocampus	Absent
TS28: globus pallidus	Absent
TS28: lateral preoptic area	Absent
TS28: main olfactory bulb external plexiform layer	Absent
TS28: medial geniculate nucleus	Absent
TS28: medial habenular nucleus	Absent
TS28: medial mammillary nucleus	Absent
TS28: medial preoptic nucleus	Absent
TS28: nucleus of lateral olfactory tract	Absent
TS28: paraventricular hypothalamic nucleus	Absent
TS28: pontine nucleus	Absent
TS28: red nucleus	Absent
TS28: reticular thalamic nucleus	Absent
TS28: suprachiasmatic nucleus	Absent
TS28: supramammillary nucleus	Absent
TS28: supraoptic nucleus	Absent
TS28: ventral posterior lateral thalamic nucleus	Absent
TS28: ventral posterior medial thalamic nucleus	Absent
TS28: ventromedial hypothalamic nucleus	Absent

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Strong
TS28: claustrum	Strong
TS28: dentate gyrus granule cell layer	Strong
TS28: dentate gyrus polymorphic layer	Strong
TS28: endopiriform nucleus	Strong
TS28: fasciola cinereum	Strong
TS28: hippocampus CA1	Strong
TS28: hippocampus CA2	Strong
TS28: hippocampus CA3	Strong
TS28: hippocampus pyramidal cell layer	Strong
TS28: hippocampus stratum oriens	Strong
TS28: indusium griseum	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: nucleus of diagonal band	Strong
TS28: piriform cortex	Strong
TS28: primary somatosensory cortex	Strong
TS28: secondary somatosensory cortex	Strong
TS28: taenia tecta	Strong
TS28: accessory olfactory bulb granule cell layer	Moderate
TS28: amygdalohippocampal area	Moderate
TS28: amygdalopiriform transition area	Moderate
TS28: anterior amygdaloid area	Moderate
TS28: basolateral amygdaloid nucleus	Moderate
TS28: basomedial amygdaloid nucleus	Moderate
TS28: bed nucleus of stria terminalis	Moderate
TS28: central amygdaloid nucleus	Moderate
TS28: cingulate cortex	Moderate
TS28: cortical layer I	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: cortical layer IV	Moderate
TS28: cortical layer V	Moderate
TS28: cortical layer VI	Moderate
TS28: dentate gyrus molecular layer	Moderate
TS28: hippocampus molecular layer	Moderate
TS28: hippocampus stratum lacunosum	Moderate
TS28: insular cortex	Moderate
TS28: lateral amygdaloid nucleus	Moderate
TS28: lateral hypothalamic area	Moderate
TS28: main olfactory bulb external plexiform layer	Moderate
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb granule cell layer	Moderate
TS28: medial amygdaloid nucleus	Moderate
TS28: medial mammillary nucleus	Moderate
TS28: nucleus reuniens	Moderate
TS28: pallidum	Moderate
TS28: periaqueductal grey	Moderate
TS28: posterior amygdaloid nucleus	Moderate
TS28: primary motor cortex	Moderate
TS28: substantia nigra	Moderate
TS28: supramammillary nucleus	Moderate
TS28: ventral tegmental area	Moderate
TS28: anterior olfactory nucleus	Moderate	Regionally restricted		Moderate in dorsal, lateral, and medial, weak in external and ventral anterior olfactory nuclei.
TS28: anterior commissure	Weak
TS28: anterior dorsal thalamic nucleus	Weak
TS28: anterior ventral thalamic nucleus	Weak
TS28: caudate-putamen	Weak
TS28: cerebellum Purkinje cell layer	Weak
TS28: corpus callosum	Weak
TS28: cortical amygdaloid nucleus	Weak
TS28: dorsal lateral geniculate nucleus	Weak
TS28: dorsal raphe nucleus	Weak
TS28: dorsomedial hypothalamic nucleus	Weak
TS28: edinger-westphal nucleus	Weak
TS28: hippocampus stratum lucidum	Weak
TS28: interpeduncular nucleus	Weak
TS28: intralaminar thalamic group	Weak
TS28: lateral habenular nucleus	Weak
TS28: lateral preoptic area	Weak
TS28: lateral septal complex	Weak
TS28: lateral thalamic group	Weak
TS28: main olfactory bulb internal plexiform layer	Weak
TS28: main olfactory bulb olfactory nerve layer	Weak
TS28: medial preoptic nucleus	Weak
TS28: medial septal complex	Weak
TS28: nucleus of lateral olfactory tract	Weak
TS28: paracentral nucleus	Weak
TS28: paraventricular hypothalamic nucleus	Weak
TS28: paraventricular thalamic nucleus	Weak
TS28: pontine reticular formation	Weak
TS28: posterolateral cortical amygdaloid nucleus	Weak
TS28: posteromedial cortical amygdaloid nucleus	Weak
TS28: reticular thalamic nucleus	Weak
TS28: septofimbrial nucleus	Weak
TS28: septohippocampal nucleus	Weak
TS28: superior colliculus	Weak
TS28: ventral lateral geniculate nucleus	Weak
TS28: ventromedial hypothalamic nucleus	Weak
TS28: zona incerta	Weak
TS28: accumbens nucleus	Absent
TS28: arcuate nucleus	Absent
TS28: brain subventricular zone	Absent
TS28: cerebellum external granule cell layer	Absent
TS28: cerebellum molecular layer	Absent
TS28: fimbria hippocampus	Absent
TS28: globus pallidus	Absent
TS28: lateral mammillary nucleus	Absent
TS28: medial geniculate nucleus	Absent
TS28: medial habenular nucleus	Absent
TS28: pontine nucleus	Absent
TS28: red nucleus	Absent
TS28: suprachiasmatic nucleus	Absent
TS28: supraoptic nucleus	Absent
TS28: ventral posterior lateral thalamic nucleus	Absent
TS28: ventral posterior medial thalamic nucleus	Absent