Probe:	Lhx5 cDNA6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Vibratome sections.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted		Expression is in the cortical hem.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1A

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1B	Expression includes the retrobulbar area and the septum.
TS20: telencephalon marginal layer	Present	Regionally restricted	1B	Expression is in the marginal zone of the pallium.
TS20: telencephalon mantle layer	Present	Regionally restricted	1B	Expression is in a domain in the dorsal part of the septum in the ventricular zone and mantle layer.
TS20: telencephalon ventricular layer	Present	Regionally restricted	1B	Expression is in a domain in the dorsal part of the septum in the ventricular zone and mantle layer.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1C	Expression includes the septum.
TS20: telencephalon marginal layer	Present	Regionally restricted	1C	Expression is in the marginal zone of the pallium.
TS20: telencephalon mantle layer	Present	Regionally restricted	1C	Expression is in a domain in the dorsal part of the septum in the ventricular zone and mantle layer.
TS20: telencephalon ventricular layer	Present	Regionally restricted	1C	Expression is in a domain in the dorsal part of the septum in the ventricular zone and mantle layer.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1D	Expression includes the septum.
TS20: telencephalon marginal layer	Present	Regionally restricted	1D	Expression is in the marginal zone of the pallium.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1E	Includes the the preoptic region.
TS20: diencephalon	Present	Regionally restricted	1E	Expression is in the alar part of prosomere 3, including the prethalamus and the eminentia thalami.
TS20: telencephalon marginal layer	Present	Regionally restricted	1E	Expression is in the marginal zone of the pallium.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1F
TS20: telencephalon marginal layer	Present	Regionally restricted	1F	Expression is in the marginal zone of the pallium.
TS20: diencephalon	Present	Regionally restricted	1F	Expression is in the alar part of prosomere 3, including the prethalamus and the eminentia thalami.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1G
TS20: telencephalon marginal layer	Present	Regionally restricted	1G	Expression is in the marginal zone of the pallium.
TS20: diencephalon	Present	Regionally restricted	1G	Expression is in the alar part of prosomere 3, including the prethalamus and the eminentia thalami.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1H	Expression is in the alar part of prosomere 3, including the prethalamus and the eminentia thalami.
TS20: telencephalon	Present	Regionally restricted	1H
TS20: hypothalamus	Present	Regionally restricted	1H	Expression is in the supraopto-paraventricular embryonic domain.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	2A	Includes the pallial septum.
TS21: telencephalon mantle layer	Present	Regionally restricted	2A	Expression is in a domain in the dorsal part of the septum in the ventricular zone and mantle layer.
TS21: telencephalon ventricular layer	Present	Regionally restricted	2A	Expression is in a domain in the dorsal part of the septum in the ventricular zone and mantle layer.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	2B	Expression is in the preoptic progenitor domains and their derivatives in the medial and/or lateral preoptic nuclei, and some related parts of the basal telencephalon.
TS22: hypothalamus	Present	Regionally restricted	2B	Expression is in the supraopto-paraventricular embryonic domain.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	2C	Expression is in the preoptic progenitor domains and their derivatives in the medial and/or lateral preoptic nuclei, and some related parts of the basal telencephalon.
TS22: hypothalamus	Present	Regionally restricted	2C	Expression is in the supraopto-paraventricular embryonic domain.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted	2D
TS24: main olfactory bulb	Weak		2D

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted	2E	Includes the pallial septum, the postmitotic cells in the septohippocampal nucleus, medial septal nucleus, and vertical limb of the diagonal band nucleus.

Structure	Level	Pattern	Image	Note
TS24: telencephalon marginal layer	Present	Regionally restricted	2F	Expression is in the marginal zone of the pallium and in the preoptic progenitor domains and their derivatives in the medial and/or lateral preoptic nuclei, and some related parts of the basal telencephalon.

Structure	Level	Pattern	Image	Note
TS24: telencephalon marginal layer	Present	Regionally restricted	2G	Expression is in the marginal zone of the pallium and in the preoptic progenitor domains and their derivatives in the medial and/or lateral preoptic nuclei, and some related parts of the basal telencephalon.
TS24: hypothalamus	Present	Regionally restricted	2G	Expression is in the supraopto-paraventricular embryonic domain.

Structure	Level	Pattern	Image	Note
TS24: telencephalon marginal layer	Present	Regionally restricted	2H	Expression is in the marginal zone of the pallium.

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	2I	Expression is in the marginal zone of the pallium, the postmitotic cells in the septohippocampal nucleus, medial septal nucleus, and vertical limb of the diagonal band nucleus.

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	2J	Expression is in the marginal zone of the pallium, the preoptic region, the postmitotic cells in the septohippocampal nucleus, medial septal nucleus, and vertical limb of the diagonal band nucleus.

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	2K	Expression is in the marginal zone of the pallium, and is strong in the basal preoptic area and derivatives, and moderately in the commissural preoptic area.

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	2L	Expression is in the marginal zone of the pallium and in the preoptic progenitor domains and their derivatives in the medial and/or lateral preoptic nuclei, and some related parts of the basal telencephalon.
TS26: hypothalamus	Present	Regionally restricted	2L	Expression is in paraventricular and supraoptic nuclei, and in some parts of the anterior hypothalamic area.

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	2M	Expression is in the marginal zone of the pallium and in the preoptic progenitor domains and their derivatives in the medial and/or lateral preoptic nuclei, and some related parts of the basal telencephalon.
TS26: hypothalamus	Present	Regionally restricted	2M	Expression is in paraventricular and supraoptic nuclei, and in some parts of the anterior hypothalamic area.

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	2N	Expression is in the marginal zone of the pallium.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	3D	Expression is in the postmitotic cells in the septohippocampal nucleus, medial septal nucleus, and vertical limb of the diagonal band nucleus.
TS22: hypothalamus	Present	Regionally restricted	3D	Expression is in the supraopto-paraventricular embryonic domain.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	3F
TS22: hypothalamus	Present	Regionally restricted	3F	Expression is in the supraopto-paraventricular embryonic domain.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	3G	Expression is in the postmitotic cells in the septohippocampal nucleus, medial septal nucleus, vertical limb of the diagonal band nucleus, and the preoptic region.
TS22: hypothalamus	Present	Regionally restricted	3G	Expression is in the supraopto-paraventricular embryonic domain.