Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS19: mesonephric tubule	Present		1A
TS19: nephric duct	Present		1A
TS19: mesonephros	Present	Regionally restricted	1A	Expression was detected in the Mullerian duct in the most anterior region of the urogenital ridge.

Structure	Level	Pattern	Image	Note
TS20: mesonephros	Present	Regionally restricted	1B	Expression in the Mullerian duct extended caudally parallel to the Wolffian duct.
TS20: mesonephric tubule	Present		1B
TS20: nephric duct	Present		1B

Structure	Level	Pattern	Image	Note
TS20: mesonephros	Present	Regionally restricted	1C	Expression was detected in the Mullerian duct along the cranial two-thirds of the urogenital ridge.
TS20: metanephros	Present		1C
TS20: mesonephric tubule	Present		1C
TS20: nephric duct	Present		1C
TS20: gonad	Absent		1C

Structure	Level	Pattern	Image	Note
TS20: mesonephric tubule	Present	Regionally restricted	1D	Expression was specifically in the epithelium and not in the surrounding mesenchyme.
TS20: nephric duct	Present	Regionally restricted	1D	Expression was specifically in the epithelium and not in the surrounding mesenchyme.
TS20: mesonephros	Present	Regionally restricted	1D	Expression was detected in the Mullerian duct epithelium, but not in the surrounding mesenchyme.
TS20: gonad	Absent		1D

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present		1E
TS21: nephric duct	Present		1E	Expression in both the Mullerian and Wolffian ducts were detected along the entire urogenital ridge.
TS21: paramesonephric duct of female	Present		1E	Expression in both the Mullerian and Wolffian ducts were detected along the entire urogenital ridge.
TS21: ovary	Absent		1E

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present		1F
TS21: paramesonephric duct of male	Present		1F	Expression in both the Mullerian and Wolffian ducts were detected along the entire urogenital ridge.
TS21: testis	Absent		1F

Structure	Level	Pattern	Image	Note
TS22: paramesonephric duct of female	Strong		2A

Structure	Level	Pattern	Image	Note
TS22: paramesonephric duct of male	Present	Regionally restricted	2B	Expression in the male Mullerian duct was thinner at E14.5 compared with females.
TS22: nephric duct	Present		2B

Structure	Level	Pattern	Image	Note
TS23: paramesonephric duct of female	Strong		2C

Structure	Level	Pattern	Image	Note
TS23: degenerating mesonephric portion of male paramesonephric duct	Present	Non-Uniform	2D	Expression in the Mullerian duct of the male becomes discontinuous from its cranial region.
TS23: epididymis	Present		2D
TS23: nephric duct of male	Present		2D

Structure	Level	Pattern	Image	Note
TS23: paramesonephric duct of female	Strong		2E
TS23: female reproductive system	Present	Regionally restricted	2E	Expression in the Wolffian duct of females was lost from the anterior gonadal region.

Structure	Level	Pattern	Image	Note
TS23: degenerating mesonephric portion of male paramesonephric duct	Present	Non-Uniform	2F	Expression in the Mullerian duct shows a fragmented pattern throughout the entire male reproductive tract region.
TS23: epididymis	Present		2F
TS23: nephric duct of male	Present		2F

Structure	Level	Pattern	Image	Note
TS24: oviduct	Weak	Regionally restricted	2G	Expression was weak in the Mullerian duct at E16.5.

Structure	Level	Pattern	Image	Note
TS24: male reproductive system	Present	Regionally restricted	2H	Expression in the Mullerian duct of males became undetectable except in a few vesicular structures.
TS24: ductus deferens	Present		2H
TS24: epididymis	Present		2H

Structure	Level	Pattern	Image	Note
TS25: oviduct	Present	Regionally restricted	2I	Expression in the Mullerian duct was restricted anteriorly to the prospective oviduct.
TS25: uterine horn	Absent		2I

Structure	Level	Pattern	Image	Note
TS25: ductus deferens	Present		2J	Expression was upregulated at E17.5.

Structure	Level	Pattern	Image	Note
TS23: degenerating mesonephric portion of male paramesonephric duct	Present	Non-Uniform	3A	Expression in the male Mullerian duct was fragmented.
TS23: male reproductive system	Present	Regionally restricted	3A	Expression was detected in the Wolffian duct.
TS23: metanephros	Present		3A

Structure	Level	Pattern	Image	Note
TS23: degenerating mesonephric portion of male paramesonephric duct	Present		3B	Expression in the male Mullerian duct was continuous in the mutant.
TS23: male reproductive system	Present	Regionally restricted	3B	Expression in the Wolffian duct was not affected in the mutant.
TS23: metanephros	Present		3B	Expression was not affected in the mutant.

Structure	Level	Pattern	Image	Note
TS23: degenerating mesonephric portion of male paramesonephric duct	Present		3C	Expression in the male Mullerian duct was continuous in the mutant.
TS23: male reproductive system	Present	Regionally restricted	3C	Expression was detected in the Wolffian duct.
TS23: metanephros	Present		3C	Expression was not affected in the mutant.

Structure	Level	Pattern	Image	Note
TS23: degenerating mesonephric portion of male paramesonephric duct	Present		3D	Expression in the male Mullerian duct was continuous in the mutant.
TS23: male reproductive system	Present	Regionally restricted	3D	Expression was detected in the Wolffian duct.
TS23: metanephros	Present		3D	Expression was not affected in the mutant.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present		3E
TS22: nephric duct	Present		3E
TS22: paramesonephric duct of female	Present		3E

Structure	Level	Pattern	Image	Note
TS23: paramesonephric duct of female	Strong		3G
TS23: metanephros	Present		3G
TS23: female reproductive system	Present	Regionally restricted	3G	Expression was detected in the Wolffian duct.

Structure	Level	Pattern	Image	Note
TS24: oviduct	Weak	Regionally restricted	3I	Expression was weak along the entire Mullerian duct in females.
TS24: uterine horn	Weak	Regionally restricted	3I	Expression was weak along the entire Mullerian duct in females.
TS24: metanephros	Present		3I
TS24: female reproductive system	Present	Regionally restricted	3I	Expression was detected in the Wolffian duct.

Structure	Level	Pattern	Image	Note
TS23: degenerating mesonephric portion of male paramesonephric duct	Present	Non-Uniform	3K	Expression in the male Mullerian duct was fragmented.

Structure	Level	Pattern	Image	Note
TS20: paramesonephric duct	Present		7A

Structure	Level	Pattern	Image	Note
TS20: mesonephros	Weak	Regionally restricted	7B	Ectopic weak staining was detected at the anterior end of the mesonephros.

Structure	Level	Pattern	Image	Note
TS20: mesonephros	Absent		7C
TS20: metanephros	Absent		7C
TS20: nephric duct	Absent		7C
TS20: paramesonephric duct	Present		7C	The shortened Mullerian duct of the mutant still showed expression.

Structure	Level	Pattern	Image	Note
TS20: genitourinary system	Present		7D	Expression was identical to wild type.
TS20: paramesonephric duct	Present		7D	Expression was identical to wild type.

Structure	Level	Pattern	Image	Note
TS19: mesonephros	Present		7E

Structure	Level	Pattern	Image	Note
TS19: mesonephros	Weak	Regionally restricted	7F	Expression was detected at the anterior end of the mesonephros of the mutant, but the positive cells remain as a small cluster rather than invaginate for tubulogenesis of the Mullerian duct.

Structure	Level	Pattern	Image	Note
TS19: mesonephros	Present		7G

Structure	Level	Pattern	Image	Note
TS19: mesonephros	Weak	Regionally restricted	7H	Expression was detected at the anterior end of the mesonephros of the mutant, but the positive cells remain as a small cluster rather than invaginate for tubulogenesis of the Mullerian duct.