Probe:

Fgf8 cDNA13

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Present			Expression was normal in the mutant at E10.0.
TS16: hindlimb bud	Present			Expression was normal in the mutant at E10.0.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Strong	Widespread	4A	Strong expression was observed throughout the apical ectodermal ridge.
TS17: hindlimb bud apical ectodermal ridge	Strong	Widespread	4A	Strong expression was observed throughout the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present	Regionally restricted	4B	There was an absence of staining in the central third of the apical ectodermal ridge frequently observed, but the pattern was highly variable.
TS17: hindlimb bud apical ectodermal ridge	Present		4B	Expression was unaltered in the hindlimb.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present	Regionally restricted	4C	Expression was absent from the majority of the apical ectodermal ridge except for the most posterior and anterior regions.
TS17: hindlimb bud apical ectodermal ridge	Present		4C	Expression was unaltered in the hindlimb.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud apical ectodermal ridge	Present	Regionally restricted	4D	In hindlimbs at E11.0, only anterior and posterior expression domains were observed in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present	Regionally restricted	4E	In forelimbs at E11.0, only the posterior expression domains were observed in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present	Patchy	4F	A nonhomogeneous expression pattern was observed with regions of no or weak expression.