Probe:	Fgf8 probe54
Probe preparation:	labelled with digoxigenin

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present	Regionally restricted	2A	Expression was detected in the anterior neural ridge.

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present	Regionally restricted	2B	The expression in the anterior neural ridge became wider in the mutant.

Structure	Level	Pattern	Image	Note
TS13: midbrain-hindbrain junction	Present		2C
TS13: future spinal cord neural plate	Present	Regionally restricted	2C	Expression was detected in the anterior neural ridge.

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present	Regionally restricted	2D	The expression in the anterior neural ridge became wider in the mutant.
TS13: midbrain-hindbrain junction	Present		2D	The expression in the isthmus became wider in the mutant.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	2E	Expression was detected in the anterior neural ridge.
TS15: midbrain-hindbrain junction	Present		2E

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	2F	The expression in the anterior neural ridge became wider in the mutant.
TS15: midbrain-hindbrain junction	Present		2F	The expression in the isthmus became wider in the mutant.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present		2G

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present		2H	The expression in the isthmus became wider in the mutant.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	2I	Expression was detected in the anterior neural ridge.
TS17: diencephalon	Present		2I
TS17: midbrain-hindbrain junction	Present		2I

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	2J	The expression in the anterior neural ridge became wider in the mutant.
TS17: midbrain-hindbrain junction	Present		2J	The expression in the isthmus became wider in the mutant.
TS17: diencephalon	Present		2J	The expression in the diencephalon became wider in the mutant.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present	Regionally restricted	2K	Expression was detected in the anterior neural ridge.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present	Regionally restricted	2L	The expression in the anterior neural ridge became wider in the mutant.

Structure	Level	Pattern	Image	Note
TS17: optic stalk	Present		3I

Structure	Level	Pattern	Image	Note
TS17: optic stalk	Present		3J	Expression in the optic stalk was expanded in the mutant.

Structure	Level	Pattern	Image	Note
TS17: head	Present	Regionally restricted	4A	Expression was detected in the epithelium of the facial primordium.

Structure	Level	Pattern	Image	Note
TS17: head	Present	Regionally restricted	4B	Expression was expanded in the facial primordium of the mutant.

Structure	Level	Pattern	Image	Note
TS17: head	Present	Regionally restricted	4C	Expression was detected in the epithelium of the facial primordium.

Structure	Level	Pattern	Image	Note
TS17: head	Present	Regionally restricted	4D	Expression was expanded in the facial primordium of the mutant.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		4K

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		4L	Expression was expanded in the apical ectodermal ridge of the mutant.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		4M

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		4N	The number of positive cells in the apical ectodermal ridge was significantly increased in the mutant (approximately 2-fold).

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	6A	Expression was detected in the anterior neural ridge.
TS17: head	Present	Regionally restricted	6A	Expression was detected in the face.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Weak	Regionally restricted	6B	Expression was very weak in the anterior neural ridge.
TS17: head	Present	Regionally restricted	6B	Expression in the facial primordia was not affected.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	6C	Expression was recovered in the anterior neural ridge.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	6D	Expression was recovered in the anterior neural ridge.
TS17: head	Present	Regionally restricted	6D	Expression was upregulated in the facial primordia.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	6E	Expression was upregulated in the anterior neural ridge.
TS17: head	Present	Regionally restricted	6E	Expression was upregulated in the facial primordia.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		6F
TS17: head	Present	Regionally restricted	6F	Expression was detected in the facial primordia.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Weak		6G	Expression was very weak in the isthmus.
TS17: head	Present	Regionally restricted	6G	Expression in the facial primordia was not affected.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		6H	Expression was recovered in the isthmus.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		6I	Expression was recovered in the isthmus.
TS17: head	Present	Regionally restricted	6I	Expression was upregulated in the facial primordia.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		6J	Expression was upregulated in the isthmus.
TS17: head	Present	Regionally restricted	6J	Expression was upregulated in the facial primordia.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		6U

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Weak		6V

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		6W	Expression was recovered in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		6X	Expression was upregulated in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		6Y	Expression was upregulated in the apical ectodermal ridge.