T RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:77516 Jiang P, et al., Targeted deletion of the MLC1f/3f downstream enhancer results in precocious MLC expression and mesoderm ablation. Dev Biol. 2002 Mar 15;243(2):281-93
Assay type:	RNA in situ
MGI Accession ID:	MGI:5501212
Gene symbol:	T
Gene name:	brachyury, T-box transcription factor T

Probe:	T cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The fixative was MEMFA (0.1 M Mops, pH 7.5, 2 mM EGTA, 1 mM MgSO4, 3.7% formaldehyde).

Results

3c normal ©

Specimen 3c normal: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		3c normal

3c mutant ©

Specimen 3c mutant: embryonic day 7.5; Myl1^tm1Nros/Myl1^tm1Nros (more

)

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Absent		3c mutant

3d normal ©

Specimen 3d normal: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		3d normal

3d mutant ©

Specimen 3d mutant: embryonic day 7.5; Myl1^tm1Nros/Myl1^tm1Nros (more

)

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Absent		3d mutant

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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