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Gene Expression Data
Assay Details
Assay
Reference: J:80521 Martin DM, et al., Pitx2 distinguishes subtypes of terminally differentiated neurons in the developing mouse neuroepithelium. Dev Biol. 2002 Dec 1;252(1):84-99
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5499445
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-Tubulin beta 3 (clone TUJ1, MMS-435P/801202)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 4A,B,C: embryonic day 10.5 (more )
Note: Double labeled: green - Tubb3; red - Pitx2 (assay MGI:5499442).
Structure Level Pattern Image Note
TS17: midbrain mantle layer Present 4B , 4C , 4A
TS17: midbrain marginal layer Absent 4B , 4C , 4A
Specimen 4D,E,F: embryonic day 10.5 (more )
Note: Double labeled: green - Tubb3; red - Pitx2 (assay MGI:5499442).
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 4F , 4D , 4E Expression is in the ventral diencephalon.
TS17: diencephalon lateral wall mantle layer Present Regionally restricted 4F , 4D , 4E Expression is in the ventral diencephalon.
Specimen 4G,H,I: embryonic day 10.5 (more )
Note: DDouble labeled: green - Tubb3; red - Pitx2 (assay MGI:5499442).
Structure Level Pattern Image Note
TS17: midbrain mantle layer Present 4G , 4H , 4I
TS17: midbrain marginal layer Absent 4H , 4G , 4I
Specimen 4J,K,L: embryonic day 10.5 (more )
Note: Double labeled: green - Tubb3; red - Pitx2 (assay MGI:5499442).
Structure Level Pattern Image Note
TS17: midbrain mantle layer Present 4L , 4J , 4K
TS17: midbrain marginal layer Absent 4J , 4L , 4K
Specimen 5A,B: embryonic day 12.5 (more )
Note: Double labeled: green - Tubb3; red - Pitx2 (assay MGI:5499442).
Structure Level Pattern Image Note
TS20: diencephalon Present 5A , 5B
TS20: midbrain Present 5A , 5B
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory