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Gene Expression Data
Assay Details
Assay
Reference: J:80521 Martin DM, et al., Pitx2 distinguishes subtypes of terminally differentiated neurons in the developing mouse neuroepithelium. Dev Biol. 2002 Dec 1;252(1):84-99
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5499442
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Antibody: P2R10
Detection system: Secondary antibody coupled to rhodamine
Results
Specimen 2A,B: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - BrdU.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 2B , 2A Expression is in the cellular nuclei of the ventral diencephalon with a gradient of decreasing label intensity from the mantle zone to the ventricular zone.
TS17: midbrain mantle layer Present 2A , 2B Expression is in the cellular nuclei.
TS17: diencephalon lateral wall marginal layer Weak 2A , 2B
TS17: midbrain marginal layer Weak 2A , 2B
Specimen 2C,D: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - phosphorylated histone.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 2D , 2C Expression is in the cellular nuclei of the ventral diencephalon with a gradient of decreasing label intensity from the mantle zone to the ventricular zone.
TS17: midbrain mantle layer Present 2C
TS17: diencephalon lateral wall marginal layer Weak 2C , 2D
TS17: midbrain marginal layer Weak 2C , 2D
Specimen 2E,F: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - BrdU.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 2E , 2F Expression is in the cellular nuclei of the ventral diencephalon with a gradient of decreasing label intensity from the mantle zone to the ventricular zone.
TS17: midbrain mantle layer Present 2E
TS17: diencephalon lateral wall marginal layer Present 2E , 2F
TS17: midbrain marginal layer Present 2E , 2F
Specimen 2G,H: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - phosphorylated histone.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 2H , 2G Expression is in the cellular nuclei of the ventral diencephalon with a gradient of decreasing label intensity from the mantle zone to the ventricular zone.
TS17: midbrain mantle layer Present 2G
TS17: diencephalon lateral wall marginal layer Weak 2G , 2H
TS17: midbrain marginal layer Weak 2G , 2H
Specimen 3A: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - BrdU.
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 3A Expression is in the alar and basal mesencephalon.
Specimen 3B: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - BrdU.
Structure Level Pattern Image Note
TS20: forebrain Present Regionally restricted 3B Expression is in the alar and basal mesencephalon.
Specimen 3C: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - BrdU.
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 3C Expression is in the alar and basal mesencephalon.
Specimen 3D: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - BrdU.
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 3D Expression is in the alar and basal mesencephalon.
TS20: diencephalon Present Regionally restricted 3D Expression is in the zona limitans intrathalamica, zona incerta/retromammillary region. In the ventral diencephalon, there are some labeled cells lining the ventricle.
Specimen 3E: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - BrdU.
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 3E Expression is in the zona limitans intrathalamica, zona incerta/retromammillary region. In the ventral diencephalon, there are some labeled cells lining the ventricle.
TS20: midbrain Present Regionally restricted 3E Expression is in the alar and basal mesencephalon.
Specimen 3F: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - BrdU.
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 3F Expression is in rhombomere 1.
TS20: diencephalon Present Regionally restricted 3F Expression is in the zona limitans intrathalamica, zona incerta/retromammillary region.
Specimen 3G: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - phosphorylated histone.
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 3G Positive staining nuclei reside near the ventricular surface.
Specimen 4A,B,C: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:5499445).
Structure Level Pattern Image Note
TS17: midbrain mantle layer Present 4B , 4A , 4C
TS17: midbrain marginal layer Present 4B , 4A , 4C
Specimen 4D,E,F: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:5499445).
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 4D , 4F , 4E Expression is in the ventral diencephalon.
TS17: diencephalon lateral wall mantle layer Present Regionally restricted 4E , 4F , 4D Expression is in the ventral diencephalon.
Specimen 4G,H,I: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:5499445).
Structure Level Pattern Image Note
TS17: midbrain mantle layer Present 4H , 4I , 4G
TS17: midbrain marginal layer Present 4H , 4I , 4G
Specimen 4J,K,L: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:5499445).
Structure Level Pattern Image Note
TS17: midbrain mantle layer Present 4K , 4L , 4J
TS17: midbrain marginal layer Present 4K , 4L , 4J
Specimen 4M: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - Map2 (assay MGI:5499452).
Structure Level Pattern Image Note
TS17: midbrain Present 4M
Specimen 5A,B: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - Tubb3 (assay MGI:5499445).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 5A , 5B
TS20: diencephalon Present Regionally restricted 5A , 5B Expression is in the zona limitans intrathalamica, zona incerta/retromammillary region.
Specimen 6A,C,D: embryonic day 10.5 (more )
Note: (C,D) are confocal images. Box in (A) is enlarged in (C), and (C) is enlarged in (D).
Structure Level Pattern Image Note
TS17: midbrain mantle layer Present Regionally restricted 6D , 6A , 6C Expression is in the ventral mesencephalon.
TS17: diencephalon Present Regionally restricted 6C , 6D , 6A Expression is in the ventral diencephalon.
Specimen 6B: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - GABA.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 6B Expression is in the ventral diencephalon.
Specimen 6E: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - GABA.
Structure Level Pattern Image Note
TS17: midbrain Present 6E
Specimen 7A,B,C,D,E: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx2; green - GABA.
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 7A , 7B Expression is in the alar and ventral mesencephalon.
TS20: diencephalon Present Regionally restricted 7C , 7D , 7A Expression is in the zona limitans intrathalamica, zona incerta/retromammillary region.
TS20: hindbrain Present Regionally restricted 7E , 7A Expression is in rhombomere 1.
Specimen 9A: embryonic day 14.5 (more )
Note: Double labeled: red - Pitx2; green - GABA.
Structure Level Pattern Image Note
TS22: diencephalon Present Regionally restricted 9A Expression is in the retromammillary region, the supramammillary nucleus, and medial mammillary nucleus.
TS22: zona incerta Present 9A
TS22: prethalamus Present 9A Expression in the subthalamic nucleus.
TS22: hypothalamus Present Regionally restricted 9A Expression in the posterior hypothalamic region.
Specimen 9B: embryonic day 14.5 (more )
Note: Double labeled: red - Pitx2; green - GABA.
Structure Level Pattern Image Note
TS22: superior colliculus Present 9B
TS22: diencephalon Present Regionally restricted 9B Expression is in the retromammillary region, the supramammillary nucleus, and medial mammillary nucleus.
TS22: zona incerta Present 9B
TS22: prethalamus Present 9B Expression in the subthalamic nucleus.
TS22: hypothalamus Present Regionally restricted 9B Expression in the posterior hypothalamic region.
Specimen 10A,G: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - Nes (assay MGI:5499446).
Structure Level Pattern Image Note
TS17: diencephalon lateral wall Strong Single cells 10A , 10G Expression is in the differentiating zone.
TS17: diencephalon lateral wall ventricular layer Weak 10G , 10A
Specimen 10B,H: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; green - Vim (assay MGI:5499448).
Structure Level Pattern Image Note
TS17: diencephalon lateral wall Strong Single cells 10B , 10H Expression is in the differentiating zone.
TS17: diencephalon lateral wall ventricular layer Weak 10H , 10B
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory